PortalPublikationenDiadenosine polyphosphates' action on calcium and vessel con...

Diadenosine polyphosphates' action on calcium and vessel contraction

Standard

Diadenosine polyphosphates' action on calcium and vessel contraction. / Tepel, M; Jankowski, J; Schlüter, H; Bachmann, J; van der Giet, M; Ruess, C; Terliesner, J; Zidek, W.

in: AM J HYPERTENS, Jahrgang 10, Nr. 12 Pt 1, 12.1997, S. 1404-10.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Tepel, M, Jankowski, J, Schlüter, H, Bachmann, J, van der Giet, M, Ruess, C, Terliesner, J & Zidek, W 1997, 'Diadenosine polyphosphates' action on calcium and vessel contraction', AM J HYPERTENS, Jg. 10, Nr. 12 Pt 1, S. 1404-10. https://doi.org/10.1016/s0895-7061(97)80305-6

APA

Tepel, M., Jankowski, J., Schlüter, H., Bachmann, J., van der Giet, M., Ruess, C., Terliesner, J., & Zidek, W. (1997). Diadenosine polyphosphates' action on calcium and vessel contraction. AM J HYPERTENS, 10(12 Pt 1), 1404-10. https://doi.org/10.1016/s0895-7061(97)80305-6

Vancouver

Tepel M, Jankowski J, Schlüter H, Bachmann J, van der Giet M, Ruess C et al. Diadenosine polyphosphates' action on calcium and vessel contraction. AM J HYPERTENS. 1997 Dez;10(12 Pt 1):1404-10. https://doi.org/10.1016/s0895-7061(97)80305-6

Bibtex

@article{63090127ffe140a68eba949671b0d689,
title = "Diadenosine polyphosphates' action on calcium and vessel contraction",
abstract = "The effects of the endogenous, platelet-derived vasoactive compounds, diadenosine tetraphosphate (AP4A), diadenosine pentaphosphate (AP5A), and diadenosine hexaphosphate (AP6A) on the vasoconstriction of isolated rat renal resistance vessels and rat aortic strips were measured using a vessel myograph. In addition, the effects of AP4A, AP5A, and AP6A on the cytosolic free calcium concentration ([Ca2+]i) were evaluated in cultured rat vascular smooth muscle cells (VSMC) using the fluorescent dye technique. Diadenosine polyphosphates dose-dependently increased the force of renal resistance vessels and isolated aortic strips. The administration of 10 mumol/L AP4A, AP5A, or AP6A significantly increased the force of isolated renal resistance vessels by 3.48+/-0.43 mN (n = 8), 2.14+/-0.40 mN (n = 12), or 2.70+/-0.31 mN (n = 11, each P < .01 compared with resting tension), respectively. The administration of 10 micromol/L AP4A, AP5A, or AP6A significantly increased the force of isolated aortic strips by 2.45+/-0.97 mNewton (n = 10), 2.70+/- 0.30 mN (n = 6), or 1.48+/-0.20 mN (each P < .01 compared with resting tension), respectively. The administration of 10 micromol/L AP4A, AP5A, or AP6A significantly increased [Ca2+]i in VSMC to a peak concentration of 314+/-60 nmol/L (n = 6), 247+/-25 nmol/L (n = 15), or 332+/-100 nmol/L (n = 5), respectively (each P < .01 compared with resting value). Both the diadenosine polyphosphate-induced vasoconstriction and [Ca2+]i increase was significantly reduced in the absence of extracellular calcium or after administration of a specific inhibitor of P2 purinoceptors. It is concluded that diadenosine polyphosphates increase [Ca2+]i and hence cause vessel constriction.",
keywords = "Animals, Calcium, Dinucleoside Phosphates, In Vitro Techniques, Male, Muscle, Smooth, Vascular, Rats, Rats, Inbred WKY, Vasoconstriction, Journal Article",
author = "M Tepel and J Jankowski and H Schl{\"u}ter and J Bachmann and {van der Giet}, M and C Ruess and J Terliesner and W Zidek",
year = "1997",
month = dec,
doi = "10.1016/s0895-7061(97)80305-6",
language = "English",
volume = "10",
pages = "1404--10",
journal = "AM J HYPERTENS",
issn = "0895-7061",
publisher = "Oxford University Press",
number = "12 Pt 1",

}

RIS

TY - JOUR

T1 - Diadenosine polyphosphates' action on calcium and vessel contraction

AU - Tepel, M

AU - Jankowski, J

AU - Schlüter, H

AU - Bachmann, J

AU - van der Giet, M

AU - Ruess, C

AU - Terliesner, J

AU - Zidek, W

PY - 1997/12

Y1 - 1997/12

N2 - The effects of the endogenous, platelet-derived vasoactive compounds, diadenosine tetraphosphate (AP4A), diadenosine pentaphosphate (AP5A), and diadenosine hexaphosphate (AP6A) on the vasoconstriction of isolated rat renal resistance vessels and rat aortic strips were measured using a vessel myograph. In addition, the effects of AP4A, AP5A, and AP6A on the cytosolic free calcium concentration ([Ca2+]i) were evaluated in cultured rat vascular smooth muscle cells (VSMC) using the fluorescent dye technique. Diadenosine polyphosphates dose-dependently increased the force of renal resistance vessels and isolated aortic strips. The administration of 10 mumol/L AP4A, AP5A, or AP6A significantly increased the force of isolated renal resistance vessels by 3.48+/-0.43 mN (n = 8), 2.14+/-0.40 mN (n = 12), or 2.70+/-0.31 mN (n = 11, each P < .01 compared with resting tension), respectively. The administration of 10 micromol/L AP4A, AP5A, or AP6A significantly increased the force of isolated aortic strips by 2.45+/-0.97 mNewton (n = 10), 2.70+/- 0.30 mN (n = 6), or 1.48+/-0.20 mN (each P < .01 compared with resting tension), respectively. The administration of 10 micromol/L AP4A, AP5A, or AP6A significantly increased [Ca2+]i in VSMC to a peak concentration of 314+/-60 nmol/L (n = 6), 247+/-25 nmol/L (n = 15), or 332+/-100 nmol/L (n = 5), respectively (each P < .01 compared with resting value). Both the diadenosine polyphosphate-induced vasoconstriction and [Ca2+]i increase was significantly reduced in the absence of extracellular calcium or after administration of a specific inhibitor of P2 purinoceptors. It is concluded that diadenosine polyphosphates increase [Ca2+]i and hence cause vessel constriction.

AB - The effects of the endogenous, platelet-derived vasoactive compounds, diadenosine tetraphosphate (AP4A), diadenosine pentaphosphate (AP5A), and diadenosine hexaphosphate (AP6A) on the vasoconstriction of isolated rat renal resistance vessels and rat aortic strips were measured using a vessel myograph. In addition, the effects of AP4A, AP5A, and AP6A on the cytosolic free calcium concentration ([Ca2+]i) were evaluated in cultured rat vascular smooth muscle cells (VSMC) using the fluorescent dye technique. Diadenosine polyphosphates dose-dependently increased the force of renal resistance vessels and isolated aortic strips. The administration of 10 mumol/L AP4A, AP5A, or AP6A significantly increased the force of isolated renal resistance vessels by 3.48+/-0.43 mN (n = 8), 2.14+/-0.40 mN (n = 12), or 2.70+/-0.31 mN (n = 11, each P < .01 compared with resting tension), respectively. The administration of 10 micromol/L AP4A, AP5A, or AP6A significantly increased the force of isolated aortic strips by 2.45+/-0.97 mNewton (n = 10), 2.70+/- 0.30 mN (n = 6), or 1.48+/-0.20 mN (each P < .01 compared with resting tension), respectively. The administration of 10 micromol/L AP4A, AP5A, or AP6A significantly increased [Ca2+]i in VSMC to a peak concentration of 314+/-60 nmol/L (n = 6), 247+/-25 nmol/L (n = 15), or 332+/-100 nmol/L (n = 5), respectively (each P < .01 compared with resting value). Both the diadenosine polyphosphate-induced vasoconstriction and [Ca2+]i increase was significantly reduced in the absence of extracellular calcium or after administration of a specific inhibitor of P2 purinoceptors. It is concluded that diadenosine polyphosphates increase [Ca2+]i and hence cause vessel constriction.

KW - Animals

KW - Calcium

KW - Dinucleoside Phosphates

KW - In Vitro Techniques

KW - Male

KW - Muscle, Smooth, Vascular

KW - Rats

KW - Rats, Inbred WKY

KW - Vasoconstriction

KW - Journal Article

U2 - 10.1016/s0895-7061(97)80305-6

DO - 10.1016/s0895-7061(97)80305-6

M3 - SCORING: Journal article

C2 - 9443777

VL - 10

SP - 1404

EP - 1410

JO - AM J HYPERTENS

JF - AM J HYPERTENS

SN - 0895-7061

IS - 12 Pt 1

ER -