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Development of Long Noncoding RNA-Based Strategies to Modulate Tissue Vascularization

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Development of Long Noncoding RNA-Based Strategies to Modulate Tissue Vascularization. / Fiedler, Jan; Breckwoldt, Kaja; Remmele, Christian W; Hartmann, Dorothee; Dittrich, Marcus; Pfanne, Angelika; Just, Annette; Xiao, Ke; Kunz, Meik; Müller, Tobias; Hansen, Arne; Geffers, Robert; Dandekar, Thomas; Eschenhagen, Thomas; Thum, Thomas.

in: J AM COLL CARDIOL, Jahrgang 66, Nr. 18, 03.11.2015, S. 2005-15.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Fiedler, J, Breckwoldt, K, Remmele, CW, Hartmann, D, Dittrich, M, Pfanne, A, Just, A, Xiao, K, Kunz, M, Müller, T, Hansen, A, Geffers, R, Dandekar, T, Eschenhagen, T & Thum, T 2015, 'Development of Long Noncoding RNA-Based Strategies to Modulate Tissue Vascularization', J AM COLL CARDIOL, Jg. 66, Nr. 18, S. 2005-15. https://doi.org/10.1016/j.jacc.2015.07.081

APA

Fiedler, J., Breckwoldt, K., Remmele, C. W., Hartmann, D., Dittrich, M., Pfanne, A., Just, A., Xiao, K., Kunz, M., Müller, T., Hansen, A., Geffers, R., Dandekar, T., Eschenhagen, T., & Thum, T. (2015). Development of Long Noncoding RNA-Based Strategies to Modulate Tissue Vascularization. J AM COLL CARDIOL, 66(18), 2005-15. https://doi.org/10.1016/j.jacc.2015.07.081

Vancouver

Fiedler J, Breckwoldt K, Remmele CW, Hartmann D, Dittrich M, Pfanne A et al. Development of Long Noncoding RNA-Based Strategies to Modulate Tissue Vascularization. J AM COLL CARDIOL. 2015 Nov 3;66(18):2005-15. https://doi.org/10.1016/j.jacc.2015.07.081

Bibtex

@article{690de60596794e71b3921e79565fbebe,
title = "Development of Long Noncoding RNA-Based Strategies to Modulate Tissue Vascularization",
abstract = "BACKGROUND: Long noncoding ribonucleic acids (lncRNAs) are a subclass of regulatory noncoding ribonucleic acids for which expression and function in human endothelial cells and angiogenic processes is not well studied.OBJECTIVES: The authors discovered hypoxia-sensitive human lncRNAs via next-generation ribonucleic acid sequencing and microarray approaches. To address their functional importance in angiogenic processes, several endothelial lncRNAs were characterized for their angiogenic characteristics in vitro and ex vivo.METHODS: Ribonucleic acid sequencing and microarray-derived data showed specific endothelial lncRNA expression changes after hypoxia. Validation experiments confirmed strong hypoxia-dependent activation of 2 intergenic lncRNAs: LINC00323 and MIR503HG.RESULTS: Silencing of these lncRNA transcripts led to angiogenic defects, including repression of growth factor signaling and/or the key endothelial transcription factor GATA2. Endothelial loss of these hypoxia-driven lncRNAs impaired cell-cycle control and inhibited capillary formation. The potential clinical importance of these endothelial lncRNAs to vascular structural integrity was demonstrated in an ex vivo model of human induced pluripotent stem cell-based engineered heart tissue.CONCLUSIONS: The authors report an expression atlas of human hypoxia-sensitive lncRNAs and identified 2 lncRNAs with important functions to sustain endothelial cell biology. LncRNAs hold great promise to serve as important future therapeutic targets of cardiovascular disease.",
keywords = "Cell Hypoxia, Cells, Cultured, Human Umbilical Vein Endothelial Cells, Humans, Hypoxia-Inducible Factor 1, alpha Subunit, Neovascularization, Pathologic, RNA Interference, RNA, Long Noncoding, Sequence Analysis, RNA, Signal Transduction, Tissue Engineering",
author = "Jan Fiedler and Kaja Breckwoldt and Remmele, {Christian W} and Dorothee Hartmann and Marcus Dittrich and Angelika Pfanne and Annette Just and Ke Xiao and Meik Kunz and Tobias M{\"u}ller and Arne Hansen and Robert Geffers and Thomas Dandekar and Thomas Eschenhagen and Thomas Thum",
note = "Copyright {\textcopyright} 2015 American College of Cardiology Foundation. Published by Elsevier Inc. All rights reserved.",
year = "2015",
month = nov,
day = "3",
doi = "10.1016/j.jacc.2015.07.081",
language = "English",
volume = "66",
pages = "2005--15",
journal = "J AM COLL CARDIOL",
issn = "0735-1097",
publisher = "Elsevier USA",
number = "18",

}

RIS

TY - JOUR

T1 - Development of Long Noncoding RNA-Based Strategies to Modulate Tissue Vascularization

AU - Fiedler, Jan

AU - Breckwoldt, Kaja

AU - Remmele, Christian W

AU - Hartmann, Dorothee

AU - Dittrich, Marcus

AU - Pfanne, Angelika

AU - Just, Annette

AU - Xiao, Ke

AU - Kunz, Meik

AU - Müller, Tobias

AU - Hansen, Arne

AU - Geffers, Robert

AU - Dandekar, Thomas

AU - Eschenhagen, Thomas

AU - Thum, Thomas

N1 - Copyright © 2015 American College of Cardiology Foundation. Published by Elsevier Inc. All rights reserved.

PY - 2015/11/3

Y1 - 2015/11/3

N2 - BACKGROUND: Long noncoding ribonucleic acids (lncRNAs) are a subclass of regulatory noncoding ribonucleic acids for which expression and function in human endothelial cells and angiogenic processes is not well studied.OBJECTIVES: The authors discovered hypoxia-sensitive human lncRNAs via next-generation ribonucleic acid sequencing and microarray approaches. To address their functional importance in angiogenic processes, several endothelial lncRNAs were characterized for their angiogenic characteristics in vitro and ex vivo.METHODS: Ribonucleic acid sequencing and microarray-derived data showed specific endothelial lncRNA expression changes after hypoxia. Validation experiments confirmed strong hypoxia-dependent activation of 2 intergenic lncRNAs: LINC00323 and MIR503HG.RESULTS: Silencing of these lncRNA transcripts led to angiogenic defects, including repression of growth factor signaling and/or the key endothelial transcription factor GATA2. Endothelial loss of these hypoxia-driven lncRNAs impaired cell-cycle control and inhibited capillary formation. The potential clinical importance of these endothelial lncRNAs to vascular structural integrity was demonstrated in an ex vivo model of human induced pluripotent stem cell-based engineered heart tissue.CONCLUSIONS: The authors report an expression atlas of human hypoxia-sensitive lncRNAs and identified 2 lncRNAs with important functions to sustain endothelial cell biology. LncRNAs hold great promise to serve as important future therapeutic targets of cardiovascular disease.

AB - BACKGROUND: Long noncoding ribonucleic acids (lncRNAs) are a subclass of regulatory noncoding ribonucleic acids for which expression and function in human endothelial cells and angiogenic processes is not well studied.OBJECTIVES: The authors discovered hypoxia-sensitive human lncRNAs via next-generation ribonucleic acid sequencing and microarray approaches. To address their functional importance in angiogenic processes, several endothelial lncRNAs were characterized for their angiogenic characteristics in vitro and ex vivo.METHODS: Ribonucleic acid sequencing and microarray-derived data showed specific endothelial lncRNA expression changes after hypoxia. Validation experiments confirmed strong hypoxia-dependent activation of 2 intergenic lncRNAs: LINC00323 and MIR503HG.RESULTS: Silencing of these lncRNA transcripts led to angiogenic defects, including repression of growth factor signaling and/or the key endothelial transcription factor GATA2. Endothelial loss of these hypoxia-driven lncRNAs impaired cell-cycle control and inhibited capillary formation. The potential clinical importance of these endothelial lncRNAs to vascular structural integrity was demonstrated in an ex vivo model of human induced pluripotent stem cell-based engineered heart tissue.CONCLUSIONS: The authors report an expression atlas of human hypoxia-sensitive lncRNAs and identified 2 lncRNAs with important functions to sustain endothelial cell biology. LncRNAs hold great promise to serve as important future therapeutic targets of cardiovascular disease.

KW - Cell Hypoxia

KW - Cells, Cultured

KW - Human Umbilical Vein Endothelial Cells

KW - Humans

KW - Hypoxia-Inducible Factor 1, alpha Subunit

KW - Neovascularization, Pathologic

KW - RNA Interference

KW - RNA, Long Noncoding

KW - Sequence Analysis, RNA

KW - Signal Transduction

KW - Tissue Engineering

U2 - 10.1016/j.jacc.2015.07.081

DO - 10.1016/j.jacc.2015.07.081

M3 - SCORING: Journal article

C2 - 26516004

VL - 66

SP - 2005

EP - 2015

JO - J AM COLL CARDIOL

JF - J AM COLL CARDIOL

SN - 0735-1097

IS - 18

ER -