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Determination of liver-specific r2 * of a highly monodisperse USPIO by (59) Fe iron core-labeling in mice at 3 T MRI

Standard

Determination of liver-specific r2 * of a highly monodisperse USPIO by (59) Fe iron core-labeling in mice at 3 T MRI. / Raabe, Nina; Forberich, Evelyn; Freund, Barbara; Bruns, Oliver T; Heine, Markus; Kaul, Michael G; Tromsdorf, Ulrich; Herich, Lena; Nielsen, Peter; Reimer, Rudolph; Hohenberg, Heinrich; Weller, Horst; Schumacher, U; Adam, Gerhard; Ittrich, Harald.

in: CONTRAST MEDIA MOL I, Jahrgang 10, 2015, S. 153-62.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Raabe, N, Forberich, E, Freund, B, Bruns, OT, Heine, M, Kaul, MG, Tromsdorf, U, Herich, L, Nielsen, P, Reimer, R, Hohenberg, H, Weller, H, Schumacher, U, Adam, G & Ittrich, H 2015, 'Determination of liver-specific r2 * of a highly monodisperse USPIO by (59) Fe iron core-labeling in mice at 3 T MRI', CONTRAST MEDIA MOL I, Jg. 10, S. 153-62. https://doi.org/10.1002/cmmi.1612

APA

Raabe, N., Forberich, E., Freund, B., Bruns, O. T., Heine, M., Kaul, M. G., Tromsdorf, U., Herich, L., Nielsen, P., Reimer, R., Hohenberg, H., Weller, H., Schumacher, U., Adam, G., & Ittrich, H. (2015). Determination of liver-specific r2 * of a highly monodisperse USPIO by (59) Fe iron core-labeling in mice at 3 T MRI. CONTRAST MEDIA MOL I, 10, 153-62. https://doi.org/10.1002/cmmi.1612

Vancouver

Raabe N, Forberich E, Freund B, Bruns OT, Heine M, Kaul MG et al. Determination of liver-specific r2 * of a highly monodisperse USPIO by (59) Fe iron core-labeling in mice at 3 T MRI. CONTRAST MEDIA MOL I. 2015;10:153-62. https://doi.org/10.1002/cmmi.1612

Bibtex

@article{0fb63250dc60467fa88a21b4d2bc11e4,
title = "Determination of liver-specific r2 * of a highly monodisperse USPIO by (59) Fe iron core-labeling in mice at 3 T MRI",
abstract = "Accurate determination of tissue concentration of ultrasmall superparamagnetic iron oxide nanoparticles (USPIO) using T2 * MR relaxometry is still challenging. We present a reliable quantification method for local USPIO amount with the estimation of the liver specific relaxivity r2 * using monodisperse (59) Fe-core-labeled USPIO ((59) FeUSPIO). Dynamic and relaxometric in vivo characteristics of unlabeled monodisperse USPIO were determined in MRI at 3 T. The in vivo MR studies were performed for liver tissue with (59) FeUSPIO using iron dosages of 9 (n = 3), 18 (n = 2) and 27 (n = 3) µmol Fe kg(-1) body weight. The R2 * of the liver before and after USPIO injection (∆R2 *) was measured and correlated with (59) Fe activity measurements of excised organs by a whole body radioactivity counter (HAMCO) to define the dependency of ∆R2 * and (59) FeUSPIO liver concentration and calculate the r2 * of (59) FeUSPIO for the liver. Ultrastructural analysis of liver uptake was performed by histology and transmission electron microscopy. ∆R2 * of the liver revealed a dosage-dependent accumulation of (59) FeUSPIO with a percentage uptake of 70-88% of the injection dose. Hepatic ∆R2 * showed a dose-dependent linear correlation to (59) FeUSPIO activity measurements (r = 0.92) and an r2 * in the liver of 481 ± 74.9 mm(-1) s(-1) in comparison to an in vitro r2 * of 60.5 ± 3.3 mm(-1) s(-1) . Our results indicate that core-labeled (59) FeUSPIO can be used to quantify the local amount of USPIO and to estimate the liver-specific relaxivity r2 *. Copyright {\textcopyright} 2014 John Wiley & Sons, Ltd.",
author = "Nina Raabe and Evelyn Forberich and Barbara Freund and Bruns, {Oliver T} and Markus Heine and Kaul, {Michael G} and Ulrich Tromsdorf and Lena Herich and Peter Nielsen and Rudolph Reimer and Heinrich Hohenberg and Horst Weller and U Schumacher and Gerhard Adam and Harald Ittrich",
note = "Copyright {\textcopyright} 2014 John Wiley & Sons, Ltd.",
year = "2015",
doi = "10.1002/cmmi.1612",
language = "English",
volume = "10",
pages = "153--62",
journal = "CONTRAST MEDIA MOL I",
issn = "1555-4309",
publisher = "John Wiley and Sons Ltd",

}

RIS

TY - JOUR

T1 - Determination of liver-specific r2 * of a highly monodisperse USPIO by (59) Fe iron core-labeling in mice at 3 T MRI

AU - Raabe, Nina

AU - Forberich, Evelyn

AU - Freund, Barbara

AU - Bruns, Oliver T

AU - Heine, Markus

AU - Kaul, Michael G

AU - Tromsdorf, Ulrich

AU - Herich, Lena

AU - Nielsen, Peter

AU - Reimer, Rudolph

AU - Hohenberg, Heinrich

AU - Weller, Horst

AU - Schumacher, U

AU - Adam, Gerhard

AU - Ittrich, Harald

N1 - Copyright © 2014 John Wiley & Sons, Ltd.

PY - 2015

Y1 - 2015

N2 - Accurate determination of tissue concentration of ultrasmall superparamagnetic iron oxide nanoparticles (USPIO) using T2 * MR relaxometry is still challenging. We present a reliable quantification method for local USPIO amount with the estimation of the liver specific relaxivity r2 * using monodisperse (59) Fe-core-labeled USPIO ((59) FeUSPIO). Dynamic and relaxometric in vivo characteristics of unlabeled monodisperse USPIO were determined in MRI at 3 T. The in vivo MR studies were performed for liver tissue with (59) FeUSPIO using iron dosages of 9 (n = 3), 18 (n = 2) and 27 (n = 3) µmol Fe kg(-1) body weight. The R2 * of the liver before and after USPIO injection (∆R2 *) was measured and correlated with (59) Fe activity measurements of excised organs by a whole body radioactivity counter (HAMCO) to define the dependency of ∆R2 * and (59) FeUSPIO liver concentration and calculate the r2 * of (59) FeUSPIO for the liver. Ultrastructural analysis of liver uptake was performed by histology and transmission electron microscopy. ∆R2 * of the liver revealed a dosage-dependent accumulation of (59) FeUSPIO with a percentage uptake of 70-88% of the injection dose. Hepatic ∆R2 * showed a dose-dependent linear correlation to (59) FeUSPIO activity measurements (r = 0.92) and an r2 * in the liver of 481 ± 74.9 mm(-1) s(-1) in comparison to an in vitro r2 * of 60.5 ± 3.3 mm(-1) s(-1) . Our results indicate that core-labeled (59) FeUSPIO can be used to quantify the local amount of USPIO and to estimate the liver-specific relaxivity r2 *. Copyright © 2014 John Wiley & Sons, Ltd.

AB - Accurate determination of tissue concentration of ultrasmall superparamagnetic iron oxide nanoparticles (USPIO) using T2 * MR relaxometry is still challenging. We present a reliable quantification method for local USPIO amount with the estimation of the liver specific relaxivity r2 * using monodisperse (59) Fe-core-labeled USPIO ((59) FeUSPIO). Dynamic and relaxometric in vivo characteristics of unlabeled monodisperse USPIO were determined in MRI at 3 T. The in vivo MR studies were performed for liver tissue with (59) FeUSPIO using iron dosages of 9 (n = 3), 18 (n = 2) and 27 (n = 3) µmol Fe kg(-1) body weight. The R2 * of the liver before and after USPIO injection (∆R2 *) was measured and correlated with (59) Fe activity measurements of excised organs by a whole body radioactivity counter (HAMCO) to define the dependency of ∆R2 * and (59) FeUSPIO liver concentration and calculate the r2 * of (59) FeUSPIO for the liver. Ultrastructural analysis of liver uptake was performed by histology and transmission electron microscopy. ∆R2 * of the liver revealed a dosage-dependent accumulation of (59) FeUSPIO with a percentage uptake of 70-88% of the injection dose. Hepatic ∆R2 * showed a dose-dependent linear correlation to (59) FeUSPIO activity measurements (r = 0.92) and an r2 * in the liver of 481 ± 74.9 mm(-1) s(-1) in comparison to an in vitro r2 * of 60.5 ± 3.3 mm(-1) s(-1) . Our results indicate that core-labeled (59) FeUSPIO can be used to quantify the local amount of USPIO and to estimate the liver-specific relaxivity r2 *. Copyright © 2014 John Wiley & Sons, Ltd.

U2 - 10.1002/cmmi.1612

DO - 10.1002/cmmi.1612

M3 - SCORING: Journal article

C2 - 25078884

VL - 10

SP - 153

EP - 162

JO - CONTRAST MEDIA MOL I

JF - CONTRAST MEDIA MOL I

SN - 1555-4309

ER -