Determination of asymmetric dimethylarginine (ADMA) using a novel ELISA assay.
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Determination of asymmetric dimethylarginine (ADMA) using a novel ELISA assay. / Schulze, Friedrich; Wesemann, Reinhard; Schwedhelm, Edzard; Sydow, Karsten; Albsmeier, Jennifer; Cooke, John P; Böger, Rainer H.
in: CLIN CHEM LAB MED, Jahrgang 42, Nr. 12, 12, 2004, S. 1377-1383.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Determination of asymmetric dimethylarginine (ADMA) using a novel ELISA assay.
AU - Schulze, Friedrich
AU - Wesemann, Reinhard
AU - Schwedhelm, Edzard
AU - Sydow, Karsten
AU - Albsmeier, Jennifer
AU - Cooke, John P
AU - Böger, Rainer H
PY - 2004
Y1 - 2004
N2 - Asymmetric dimethylarginine (ADMA) is an endogenous competitive inhibitor of nitric oxide synthase (NOS). Elevated ADMA plasma levels have been reported in connection with diseases associated with an impaired endothelial L-arginine-NO pathway and endothelial dysfunction, such as atherosclerosis, hypercholesterolemia, chronic heart failure, diabetes mellitus, and hypertension. NO production by NOS is decreased due to elevated ADMA levels. In fact, there is increasing interest in determination of ADMA levels in samples of various origins. The aim of this work was to develop a precise and easy immunoassay in contrast to the existing methods, such as HPLC, liquid chromatography-mass spectrometry (LC-MS) and gas chromatography (GC)-MS. We determined cross-reactivity in our immunoassay of 1.2% for symmetric dimethylarginine and
AB - Asymmetric dimethylarginine (ADMA) is an endogenous competitive inhibitor of nitric oxide synthase (NOS). Elevated ADMA plasma levels have been reported in connection with diseases associated with an impaired endothelial L-arginine-NO pathway and endothelial dysfunction, such as atherosclerosis, hypercholesterolemia, chronic heart failure, diabetes mellitus, and hypertension. NO production by NOS is decreased due to elevated ADMA levels. In fact, there is increasing interest in determination of ADMA levels in samples of various origins. The aim of this work was to develop a precise and easy immunoassay in contrast to the existing methods, such as HPLC, liquid chromatography-mass spectrometry (LC-MS) and gas chromatography (GC)-MS. We determined cross-reactivity in our immunoassay of 1.2% for symmetric dimethylarginine and
M3 - SCORING: Zeitschriftenaufsatz
VL - 42
SP - 1377
EP - 1383
JO - CLIN CHEM LAB MED
JF - CLIN CHEM LAB MED
SN - 1434-6621
IS - 12
M1 - 12
ER -
