Detection of squamous cell carcinoma of the oral cavity by imaging 5-aminolevulinic acid-induced protoporphyrin IX fluorescence
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Detection of squamous cell carcinoma of the oral cavity by imaging 5-aminolevulinic acid-induced protoporphyrin IX fluorescence. / Leunig, A; Betz, CS; Mehlmann, M; Stepp, H; Arbogast, S; Grevers, G; Baumgartner, R.
in: LARYNGOSCOPE, Jahrgang 110, Nr. 1, 01.01.2000, S. 78-83.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Detection of squamous cell carcinoma of the oral cavity by imaging 5-aminolevulinic acid-induced protoporphyrin IX fluorescence
AU - Leunig, A
AU - Betz, CS
AU - Mehlmann, M
AU - Stepp, H
AU - Arbogast, S
AU - Grevers, G
AU - Baumgartner, R
N1 - 4th International Symposium on Predictive Oncology and Theray, NICE, FRANCE, OCT 24-27, 1998
PY - 2000/1/1
Y1 - 2000/1/1
N2 - Objectives Early cancer detection is the best way to improve the prognosis of patients with oral cancer. Therefore this study presents quantitative fluorescence measurements and results in the visualization of cancerous oral mucosa with 5-aminolevulinic acid (5-ALA)-induced protoporphyrin IX (PPM). Methods: Time progression and type of porphyrin accumulation were analyzed in neoplastic and surrounding healthy tissue of 58 patients with a suspected cancer of the oral cavity by measuring emission spectra of 5-ALA-induced PPIX fluorescence, Fluorescence images in the red and green spectral range from the tumor tissue were recorded with a charge-coupled device camera, Results: After topical application of 0.4% 5-ALA and incubation for 1 to 2.5 hours, all patients revealed higher intensities of red fluorescence in neoplastic tissue compared with the surrounding normal tissue. Maximum contrast was reached after 1.5 hours of incubation. In 13.8% (n = 8) of the patients, additional findings like dysplasia, carcinoma in situ, primary tumor, secondary carcinomas, and tumor branches were found by means of fluorescence marking in contrast to white light examination. An evaluation of the biopsy specimens resulted in a specificity of 60% and a sensitivity of 99 Conclusions: As a fluorescent marker, PPM could represent a possible new diagnostic tool to detect early malignant and secondary lesions in the oral cavity, In addition, 5-ALA-induced PPM fluorescence is promising as a useful intraoperative tool for determining adequate surgical margins of resection, Further investigations aim to assess this diagnostic procedure as a sensitive and clinically reliable method for patients with oral cancer.
AB - Objectives Early cancer detection is the best way to improve the prognosis of patients with oral cancer. Therefore this study presents quantitative fluorescence measurements and results in the visualization of cancerous oral mucosa with 5-aminolevulinic acid (5-ALA)-induced protoporphyrin IX (PPM). Methods: Time progression and type of porphyrin accumulation were analyzed in neoplastic and surrounding healthy tissue of 58 patients with a suspected cancer of the oral cavity by measuring emission spectra of 5-ALA-induced PPIX fluorescence, Fluorescence images in the red and green spectral range from the tumor tissue were recorded with a charge-coupled device camera, Results: After topical application of 0.4% 5-ALA and incubation for 1 to 2.5 hours, all patients revealed higher intensities of red fluorescence in neoplastic tissue compared with the surrounding normal tissue. Maximum contrast was reached after 1.5 hours of incubation. In 13.8% (n = 8) of the patients, additional findings like dysplasia, carcinoma in situ, primary tumor, secondary carcinomas, and tumor branches were found by means of fluorescence marking in contrast to white light examination. An evaluation of the biopsy specimens resulted in a specificity of 60% and a sensitivity of 99 Conclusions: As a fluorescent marker, PPM could represent a possible new diagnostic tool to detect early malignant and secondary lesions in the oral cavity, In addition, 5-ALA-induced PPM fluorescence is promising as a useful intraoperative tool for determining adequate surgical margins of resection, Further investigations aim to assess this diagnostic procedure as a sensitive and clinically reliable method for patients with oral cancer.
KW - cancer
KW - oral cavity
KW - 5-aminolevulinic acid
KW - protoporphyrin IX
KW - fluorescence
KW - diagnosis
U2 - 10.1097/00005537-200001000-00015
DO - 10.1097/00005537-200001000-00015
M3 - SCORING: Journal article
VL - 110
SP - 78
EP - 83
JO - LARYNGOSCOPE
JF - LARYNGOSCOPE
SN - 0023-852X
IS - 1
ER -