Detection of Calcified Aortic Plaques in an Apolipoprotein E Animal Model Using a Human Computed Tomography System for Ultra-High-resolution Imaging
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Detection of Calcified Aortic Plaques in an Apolipoprotein E Animal Model Using a Human Computed Tomography System for Ultra-High-resolution Imaging : A Feasibility Study. / Manta, Calin; Meyer, Mathias; Géraud, Cyrill; Roscher, Mareike; Goerdt, Sergij; Schoenberg, Stefan O; Henzler, Thomas; Ong, Melissa M.
in: J THORAC IMAG, Jahrgang 34, Nr. 1, 01.2019, S. 41-47.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Detection of Calcified Aortic Plaques in an Apolipoprotein E Animal Model Using a Human Computed Tomography System for Ultra-High-resolution Imaging
T2 - A Feasibility Study
AU - Manta, Calin
AU - Meyer, Mathias
AU - Géraud, Cyrill
AU - Roscher, Mareike
AU - Goerdt, Sergij
AU - Schoenberg, Stefan O
AU - Henzler, Thomas
AU - Ong, Melissa M
PY - 2019/1
Y1 - 2019/1
N2 - PURPOSE: The purpose of this study was to prospectively evaluate the feasibility of detecting calcified aortic plaques in apolipoprotein E knockout (ApoE-KO) mice using a state-of-the-art human computed tomography (CT) system.MATERIALS AND METHODS: Eleven ApoE-KO and 9 wild-type mice, all male, were examined in this study. After intraperitoneal injection of 6.6% ketamine all mice underwent 2 ultra-high-resolution CT protocols on a third-generation dual-source CT system at 120 kVp and 130 kVp tube voltage, both performed with a tube current time product of 1300 mAs. Images (0.4 mm) with an increment of 0.2 mm were reconstructed using an iterative reconstruction algorithm. Calcium detectability and scores (Agatston, volume, mass) were determined with a dedicated human calcium scoring software (CaScoring). After the CT examination, a calcium quantification assay of the aortae was performed to determine the aortic calcium content of each mouse. The CT scan time ranged between 40 and 48 seconds. All mice survived the procedure.RESULTS: Calcified plaques could be detected in 8 of 11 ApoE-KO mice. Quantification of calcium levels showed significant differences between those with morphologic calcium plaques detected in CT and those without (3.44±1.6 μg Ca/mg vs. 0.33±0.35 μg Ca/mg; P<0.05). The receiver-operating characteristics analysis revealed a total calcium cut-off value of 0.71 μg Ca/mg for the detection using calcium score algorithms (specificity: 100% and sensitivity: 90%).CONCLUSION: Using a state-of-the-art human CT protocol and an in-human-established calcium scoring system allows for the detection and quantification of calcified aortic plaques in ApoE-KO mice. These results may facilitate preclinical imaging for translational and longitudinal atherosclerotic research studies.
AB - PURPOSE: The purpose of this study was to prospectively evaluate the feasibility of detecting calcified aortic plaques in apolipoprotein E knockout (ApoE-KO) mice using a state-of-the-art human computed tomography (CT) system.MATERIALS AND METHODS: Eleven ApoE-KO and 9 wild-type mice, all male, were examined in this study. After intraperitoneal injection of 6.6% ketamine all mice underwent 2 ultra-high-resolution CT protocols on a third-generation dual-source CT system at 120 kVp and 130 kVp tube voltage, both performed with a tube current time product of 1300 mAs. Images (0.4 mm) with an increment of 0.2 mm were reconstructed using an iterative reconstruction algorithm. Calcium detectability and scores (Agatston, volume, mass) were determined with a dedicated human calcium scoring software (CaScoring). After the CT examination, a calcium quantification assay of the aortae was performed to determine the aortic calcium content of each mouse. The CT scan time ranged between 40 and 48 seconds. All mice survived the procedure.RESULTS: Calcified plaques could be detected in 8 of 11 ApoE-KO mice. Quantification of calcium levels showed significant differences between those with morphologic calcium plaques detected in CT and those without (3.44±1.6 μg Ca/mg vs. 0.33±0.35 μg Ca/mg; P<0.05). The receiver-operating characteristics analysis revealed a total calcium cut-off value of 0.71 μg Ca/mg for the detection using calcium score algorithms (specificity: 100% and sensitivity: 90%).CONCLUSION: Using a state-of-the-art human CT protocol and an in-human-established calcium scoring system allows for the detection and quantification of calcified aortic plaques in ApoE-KO mice. These results may facilitate preclinical imaging for translational and longitudinal atherosclerotic research studies.
KW - Animals
KW - Apolipoproteins E/deficiency
KW - Computed Tomography Angiography/methods
KW - Coronary Angiography/methods
KW - Coronary Artery Disease/diagnostic imaging
KW - Disease Models, Animal
KW - Feasibility Studies
KW - Male
KW - Mice, Inbred C57BL
KW - Plaque, Atherosclerotic/diagnostic imaging
KW - Prospective Studies
U2 - 10.1097/RTI.0000000000000375
DO - 10.1097/RTI.0000000000000375
M3 - SCORING: Journal article
C2 - 30480591
VL - 34
SP - 41
EP - 47
JO - J THORAC IMAG
JF - J THORAC IMAG
SN - 0883-5993
IS - 1
ER -