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Detection of angiotensin II in supernatants of stimulated mononuclear leukocytes by matrix-assisted laser desorption ionization time-of-flight/time-of-flight mass analysis

Standard

Detection of angiotensin II in supernatants of stimulated mononuclear leukocytes by matrix-assisted laser desorption ionization time-of-flight/time-of-flight mass analysis. / Jankowski, Vera; Vanholder, Raymond; van der Giet, Markus; Henning, Lars; Tölle, Markus; Schönfelder, Gilbert; Krakow, Anja; Karadogan, Sevil; Gustavsson, Niklas; Gobom, Johan; Webb, Jessie; Lehrach, Hans; Giebing, Günther; Schlüter, Hartmut; Hilgers, Karl F; Zidek, Walter; Jankowski, Joachim.

in: HYPERTENSION, Jahrgang 46, Nr. 3, 09.2005, S. 591-7.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Jankowski, V, Vanholder, R, van der Giet, M, Henning, L, Tölle, M, Schönfelder, G, Krakow, A, Karadogan, S, Gustavsson, N, Gobom, J, Webb, J, Lehrach, H, Giebing, G, Schlüter, H, Hilgers, KF, Zidek, W & Jankowski, J 2005, 'Detection of angiotensin II in supernatants of stimulated mononuclear leukocytes by matrix-assisted laser desorption ionization time-of-flight/time-of-flight mass analysis', HYPERTENSION, Jg. 46, Nr. 3, S. 591-7. https://doi.org/10.1161/01.HYP.0000177436.09733.d4

APA

Jankowski, V., Vanholder, R., van der Giet, M., Henning, L., Tölle, M., Schönfelder, G., Krakow, A., Karadogan, S., Gustavsson, N., Gobom, J., Webb, J., Lehrach, H., Giebing, G., Schlüter, H., Hilgers, K. F., Zidek, W., & Jankowski, J. (2005). Detection of angiotensin II in supernatants of stimulated mononuclear leukocytes by matrix-assisted laser desorption ionization time-of-flight/time-of-flight mass analysis. HYPERTENSION, 46(3), 591-7. https://doi.org/10.1161/01.HYP.0000177436.09733.d4

Vancouver

Jankowski V, Vanholder R, van der Giet M, Henning L, Tölle M, Schönfelder G et al. Detection of angiotensin II in supernatants of stimulated mononuclear leukocytes by matrix-assisted laser desorption ionization time-of-flight/time-of-flight mass analysis. HYPERTENSION. 2005 Sep;46(3):591-7. https://doi.org/10.1161/01.HYP.0000177436.09733.d4

Bibtex

@article{cfc50464673a4c9f895f9b42a58a97a5,
title = "Detection of angiotensin II in supernatants of stimulated mononuclear leukocytes by matrix-assisted laser desorption ionization time-of-flight/time-of-flight mass analysis",
abstract = "Angiotensin II (Ang II) is the major vasoactive component of the renin-angiotensin system. Several components of the renin-angiotensin system have been demonstrated in different tissues. Whereas the roles of tissue and renal renin-angiotensin system have been studied in detail, much less is known on whether the corpuscular elements of circulating blood contribute to Ang II production. Here we examined whether, in addition to vasculature, blood cells also contribute to the circulating Ang II levels. Mononuclear leukocytes were obtained from healthy subjects and were incubated. The resulting supernatant was chromatographed using different chromatographic methods. The vasoconstrictive effects of aliquots of the resulting fractions were tested. Each fraction with a vasoconstrictive effect was analyzed by mass spectrometry. In one fraction with a strong vasoconstrictive effect, Ang II was identified. Mononuclear lymphocytes produced Ang II in amounts sufficient to stimulate Ang II type 1 receptors. Moreover, in mononuclear leukocytes, renin as well as angiotensin-converting enzyme mRNA expression was detectable by RT-PCR. These findings demonstrate that mononuclear leukocytes are a source of Ang II. Ang II secretion by these cells may play a significant role in humoral vascular regulation. In conclusion, the isolation of Ang II in supernatants of mononuclear leukocytes adds a further physiological source of Ang II to the current view of angiotensin metabolism. The quantitative role of lymphocyte-derived Ang II secretion compared with the other sources of Ang II should be defined further, but the release found under the present conditions is at least sufficient to elicit vasoconstrictive effects.",
keywords = "Angiotensin II, Animals, Blood Vessels, Humans, In Vitro Techniques, Kidney, Monocytes, Peptidyl-Dipeptidase A, RNA, Messenger, Rats, Receptor, Angiotensin, Type 1, Renin, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Vasoconstriction, Vasoconstrictor Agents, Journal Article",
author = "Vera Jankowski and Raymond Vanholder and {van der Giet}, Markus and Lars Henning and Markus T{\"o}lle and Gilbert Sch{\"o}nfelder and Anja Krakow and Sevil Karadogan and Niklas Gustavsson and Johan Gobom and Jessie Webb and Hans Lehrach and G{\"u}nther Giebing and Hartmut Schl{\"u}ter and Hilgers, {Karl F} and Walter Zidek and Joachim Jankowski",
year = "2005",
month = sep,
doi = "10.1161/01.HYP.0000177436.09733.d4",
language = "English",
volume = "46",
pages = "591--7",
journal = "HYPERTENSION",
issn = "0194-911X",
publisher = "Lippincott Williams and Wilkins",
number = "3",

}

RIS

TY - JOUR

T1 - Detection of angiotensin II in supernatants of stimulated mononuclear leukocytes by matrix-assisted laser desorption ionization time-of-flight/time-of-flight mass analysis

AU - Jankowski, Vera

AU - Vanholder, Raymond

AU - van der Giet, Markus

AU - Henning, Lars

AU - Tölle, Markus

AU - Schönfelder, Gilbert

AU - Krakow, Anja

AU - Karadogan, Sevil

AU - Gustavsson, Niklas

AU - Gobom, Johan

AU - Webb, Jessie

AU - Lehrach, Hans

AU - Giebing, Günther

AU - Schlüter, Hartmut

AU - Hilgers, Karl F

AU - Zidek, Walter

AU - Jankowski, Joachim

PY - 2005/9

Y1 - 2005/9

N2 - Angiotensin II (Ang II) is the major vasoactive component of the renin-angiotensin system. Several components of the renin-angiotensin system have been demonstrated in different tissues. Whereas the roles of tissue and renal renin-angiotensin system have been studied in detail, much less is known on whether the corpuscular elements of circulating blood contribute to Ang II production. Here we examined whether, in addition to vasculature, blood cells also contribute to the circulating Ang II levels. Mononuclear leukocytes were obtained from healthy subjects and were incubated. The resulting supernatant was chromatographed using different chromatographic methods. The vasoconstrictive effects of aliquots of the resulting fractions were tested. Each fraction with a vasoconstrictive effect was analyzed by mass spectrometry. In one fraction with a strong vasoconstrictive effect, Ang II was identified. Mononuclear lymphocytes produced Ang II in amounts sufficient to stimulate Ang II type 1 receptors. Moreover, in mononuclear leukocytes, renin as well as angiotensin-converting enzyme mRNA expression was detectable by RT-PCR. These findings demonstrate that mononuclear leukocytes are a source of Ang II. Ang II secretion by these cells may play a significant role in humoral vascular regulation. In conclusion, the isolation of Ang II in supernatants of mononuclear leukocytes adds a further physiological source of Ang II to the current view of angiotensin metabolism. The quantitative role of lymphocyte-derived Ang II secretion compared with the other sources of Ang II should be defined further, but the release found under the present conditions is at least sufficient to elicit vasoconstrictive effects.

AB - Angiotensin II (Ang II) is the major vasoactive component of the renin-angiotensin system. Several components of the renin-angiotensin system have been demonstrated in different tissues. Whereas the roles of tissue and renal renin-angiotensin system have been studied in detail, much less is known on whether the corpuscular elements of circulating blood contribute to Ang II production. Here we examined whether, in addition to vasculature, blood cells also contribute to the circulating Ang II levels. Mononuclear leukocytes were obtained from healthy subjects and were incubated. The resulting supernatant was chromatographed using different chromatographic methods. The vasoconstrictive effects of aliquots of the resulting fractions were tested. Each fraction with a vasoconstrictive effect was analyzed by mass spectrometry. In one fraction with a strong vasoconstrictive effect, Ang II was identified. Mononuclear lymphocytes produced Ang II in amounts sufficient to stimulate Ang II type 1 receptors. Moreover, in mononuclear leukocytes, renin as well as angiotensin-converting enzyme mRNA expression was detectable by RT-PCR. These findings demonstrate that mononuclear leukocytes are a source of Ang II. Ang II secretion by these cells may play a significant role in humoral vascular regulation. In conclusion, the isolation of Ang II in supernatants of mononuclear leukocytes adds a further physiological source of Ang II to the current view of angiotensin metabolism. The quantitative role of lymphocyte-derived Ang II secretion compared with the other sources of Ang II should be defined further, but the release found under the present conditions is at least sufficient to elicit vasoconstrictive effects.

KW - Angiotensin II

KW - Animals

KW - Blood Vessels

KW - Humans

KW - In Vitro Techniques

KW - Kidney

KW - Monocytes

KW - Peptidyl-Dipeptidase A

KW - RNA, Messenger

KW - Rats

KW - Receptor, Angiotensin, Type 1

KW - Renin

KW - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

KW - Vasoconstriction

KW - Vasoconstrictor Agents

KW - Journal Article

U2 - 10.1161/01.HYP.0000177436.09733.d4

DO - 10.1161/01.HYP.0000177436.09733.d4

M3 - SCORING: Journal article

C2 - 16087781

VL - 46

SP - 591

EP - 597

JO - HYPERTENSION

JF - HYPERTENSION

SN - 0194-911X

IS - 3

ER -