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Delta-Like Ligand 4 Modulates Liver Damage by Down-Regulating Chemokine Expression

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Delta-Like Ligand 4 Modulates Liver Damage by Down-Regulating Chemokine Expression. / Shen, Zhe; Liu, Yan; Dewidar, Bedair; Hu, Junhao; Park, Ogyi; Feng, Teng; Xu, Chengfu; Yu, Chaohui; Li, Qi; Meyer, Christoph; Ilkavets, Iryna; Müller, Alexandra; Stump-Guthier, Carolin; Munker, Stefan; Liebe, Roman; Zimmer, Vincent; Lammert, Frank; Mertens, Peter R; Li, Hai; Ten Dijke, Peter; Augustin, Hellmut G; Li, Jun; Gao, Bin; Ebert, Matthias P; Dooley, Steven; Li, Youming; Weng, Hong-Lei.

in: AM J PATHOL, Jahrgang 186, Nr. 7, 07.2016, S. 1874-89.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Shen, Z, Liu, Y, Dewidar, B, Hu, J, Park, O, Feng, T, Xu, C, Yu, C, Li, Q, Meyer, C, Ilkavets, I, Müller, A, Stump-Guthier, C, Munker, S, Liebe, R, Zimmer, V, Lammert, F, Mertens, PR, Li, H, Ten Dijke, P, Augustin, HG, Li, J, Gao, B, Ebert, MP, Dooley, S, Li, Y & Weng, H-L 2016, 'Delta-Like Ligand 4 Modulates Liver Damage by Down-Regulating Chemokine Expression', AM J PATHOL, Jg. 186, Nr. 7, S. 1874-89. https://doi.org/10.1016/j.ajpath.2016.03.010

APA

Shen, Z., Liu, Y., Dewidar, B., Hu, J., Park, O., Feng, T., Xu, C., Yu, C., Li, Q., Meyer, C., Ilkavets, I., Müller, A., Stump-Guthier, C., Munker, S., Liebe, R., Zimmer, V., Lammert, F., Mertens, P. R., Li, H., ... Weng, H-L. (2016). Delta-Like Ligand 4 Modulates Liver Damage by Down-Regulating Chemokine Expression. AM J PATHOL, 186(7), 1874-89. https://doi.org/10.1016/j.ajpath.2016.03.010

Vancouver

Shen Z, Liu Y, Dewidar B, Hu J, Park O, Feng T et al. Delta-Like Ligand 4 Modulates Liver Damage by Down-Regulating Chemokine Expression. AM J PATHOL. 2016 Jul;186(7):1874-89. https://doi.org/10.1016/j.ajpath.2016.03.010

Bibtex

@article{11a6f68c648641f2bfae645175a3cfd3,
title = "Delta-Like Ligand 4 Modulates Liver Damage by Down-Regulating Chemokine Expression",
abstract = "Disrupting Notch signaling ameliorates experimental liver fibrosis. However, the role of individual Notch ligands in liver damage is unknown. We investigated the effects of Delta-like ligand 4 (Dll4) in liver disease. DLL4 expression was measured in 31 human liver tissues by immunohistochemistry. Dll4 function was examined in carbon tetrachloride- and bile duct ligation-challenged mouse models in vivo and evaluated in hepatic stellate cells, hepatocytes, and Kupffer cells in vitro. DLL4 was expressed in patients' Kupffer and liver sinusoidal endothelial cells. Recombinant Dll4 protein (rDll4) ameliorated hepatocyte apoptosis, inflammation, and fibrosis in mice after carbon tetrachloride challenge. In vitro, rDll4 significantly decreased lipopolysaccharide-dependent chemokine expression in both Kupffer and hepatic stellate cells. In bile duct ligation mice, rDll4 induced massive hepatic necrosis, resulting in the death of all animals within 1 week. Inflammatory cell infiltration and chemokine ligand 2 (Ccl2) expression were significantly reduced in rDll4-receiving bile duct ligation mice. Recombinant Ccl2 rescued bile duct ligation mice from rDll4-mediated death. In patients with acute-on-chronic liver failure, DLL4 expression was inversely associated with CCL2 abundance. Mechanistically, Dll4 regulated Ccl2 expression via NF-κB. Taken together, Dll4 modulates liver inflammatory response by down-regulating chemokine expression. rDll4 application results in opposing outcomes in two models of liver damage. Loss of DLL4 may be associated with CCL2-mediated cytokine storm in patients with acute-on-chronic liver failure.",
keywords = "Journal Article",
author = "Zhe Shen and Yan Liu and Bedair Dewidar and Junhao Hu and Ogyi Park and Teng Feng and Chengfu Xu and Chaohui Yu and Qi Li and Christoph Meyer and Iryna Ilkavets and Alexandra M{\"u}ller and Carolin Stump-Guthier and Stefan Munker and Roman Liebe and Vincent Zimmer and Frank Lammert and Mertens, {Peter R} and Hai Li and {Ten Dijke}, Peter and Augustin, {Hellmut G} and Jun Li and Bin Gao and Ebert, {Matthias P} and Steven Dooley and Youming Li and Hong-Lei Weng",
note = "Copyright {\textcopyright} 2016 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.",
year = "2016",
month = jul,
doi = "10.1016/j.ajpath.2016.03.010",
language = "English",
volume = "186",
pages = "1874--89",
journal = "AM J PATHOL",
issn = "0002-9440",
publisher = "Elsevier Inc.",
number = "7",

}

RIS

TY - JOUR

T1 - Delta-Like Ligand 4 Modulates Liver Damage by Down-Regulating Chemokine Expression

AU - Shen, Zhe

AU - Liu, Yan

AU - Dewidar, Bedair

AU - Hu, Junhao

AU - Park, Ogyi

AU - Feng, Teng

AU - Xu, Chengfu

AU - Yu, Chaohui

AU - Li, Qi

AU - Meyer, Christoph

AU - Ilkavets, Iryna

AU - Müller, Alexandra

AU - Stump-Guthier, Carolin

AU - Munker, Stefan

AU - Liebe, Roman

AU - Zimmer, Vincent

AU - Lammert, Frank

AU - Mertens, Peter R

AU - Li, Hai

AU - Ten Dijke, Peter

AU - Augustin, Hellmut G

AU - Li, Jun

AU - Gao, Bin

AU - Ebert, Matthias P

AU - Dooley, Steven

AU - Li, Youming

AU - Weng, Hong-Lei

N1 - Copyright © 2016 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.

PY - 2016/7

Y1 - 2016/7

N2 - Disrupting Notch signaling ameliorates experimental liver fibrosis. However, the role of individual Notch ligands in liver damage is unknown. We investigated the effects of Delta-like ligand 4 (Dll4) in liver disease. DLL4 expression was measured in 31 human liver tissues by immunohistochemistry. Dll4 function was examined in carbon tetrachloride- and bile duct ligation-challenged mouse models in vivo and evaluated in hepatic stellate cells, hepatocytes, and Kupffer cells in vitro. DLL4 was expressed in patients' Kupffer and liver sinusoidal endothelial cells. Recombinant Dll4 protein (rDll4) ameliorated hepatocyte apoptosis, inflammation, and fibrosis in mice after carbon tetrachloride challenge. In vitro, rDll4 significantly decreased lipopolysaccharide-dependent chemokine expression in both Kupffer and hepatic stellate cells. In bile duct ligation mice, rDll4 induced massive hepatic necrosis, resulting in the death of all animals within 1 week. Inflammatory cell infiltration and chemokine ligand 2 (Ccl2) expression were significantly reduced in rDll4-receiving bile duct ligation mice. Recombinant Ccl2 rescued bile duct ligation mice from rDll4-mediated death. In patients with acute-on-chronic liver failure, DLL4 expression was inversely associated with CCL2 abundance. Mechanistically, Dll4 regulated Ccl2 expression via NF-κB. Taken together, Dll4 modulates liver inflammatory response by down-regulating chemokine expression. rDll4 application results in opposing outcomes in two models of liver damage. Loss of DLL4 may be associated with CCL2-mediated cytokine storm in patients with acute-on-chronic liver failure.

AB - Disrupting Notch signaling ameliorates experimental liver fibrosis. However, the role of individual Notch ligands in liver damage is unknown. We investigated the effects of Delta-like ligand 4 (Dll4) in liver disease. DLL4 expression was measured in 31 human liver tissues by immunohistochemistry. Dll4 function was examined in carbon tetrachloride- and bile duct ligation-challenged mouse models in vivo and evaluated in hepatic stellate cells, hepatocytes, and Kupffer cells in vitro. DLL4 was expressed in patients' Kupffer and liver sinusoidal endothelial cells. Recombinant Dll4 protein (rDll4) ameliorated hepatocyte apoptosis, inflammation, and fibrosis in mice after carbon tetrachloride challenge. In vitro, rDll4 significantly decreased lipopolysaccharide-dependent chemokine expression in both Kupffer and hepatic stellate cells. In bile duct ligation mice, rDll4 induced massive hepatic necrosis, resulting in the death of all animals within 1 week. Inflammatory cell infiltration and chemokine ligand 2 (Ccl2) expression were significantly reduced in rDll4-receiving bile duct ligation mice. Recombinant Ccl2 rescued bile duct ligation mice from rDll4-mediated death. In patients with acute-on-chronic liver failure, DLL4 expression was inversely associated with CCL2 abundance. Mechanistically, Dll4 regulated Ccl2 expression via NF-κB. Taken together, Dll4 modulates liver inflammatory response by down-regulating chemokine expression. rDll4 application results in opposing outcomes in two models of liver damage. Loss of DLL4 may be associated with CCL2-mediated cytokine storm in patients with acute-on-chronic liver failure.

KW - Journal Article

U2 - 10.1016/j.ajpath.2016.03.010

DO - 10.1016/j.ajpath.2016.03.010

M3 - SCORING: Journal article

C2 - 27171900

VL - 186

SP - 1874

EP - 1889

JO - AM J PATHOL

JF - AM J PATHOL

SN - 0002-9440

IS - 7

ER -