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Cytoskeleton architecture of C6 rat glioma cell subclones differing in intermediate filament protein expression

Standard

Cytoskeleton architecture of C6 rat glioma cell subclones differing in intermediate filament protein expression. / Bohn, W; Röser, K; Hohenberg, H; Mannweiler, K; Traub, P.

in: J STRUCT BIOL, Jahrgang 111, Nr. 1, 01.07.1993, S. 48-58.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Bohn, W, Röser, K, Hohenberg, H, Mannweiler, K & Traub, P 1993, 'Cytoskeleton architecture of C6 rat glioma cell subclones differing in intermediate filament protein expression', J STRUCT BIOL, Jg. 111, Nr. 1, S. 48-58. https://doi.org/10.1006/jsbi.1993.1035

APA

Bohn, W., Röser, K., Hohenberg, H., Mannweiler, K., & Traub, P. (1993). Cytoskeleton architecture of C6 rat glioma cell subclones differing in intermediate filament protein expression. J STRUCT BIOL, 111(1), 48-58. https://doi.org/10.1006/jsbi.1993.1035

Vancouver

Bohn W, Röser K, Hohenberg H, Mannweiler K, Traub P. Cytoskeleton architecture of C6 rat glioma cell subclones differing in intermediate filament protein expression. J STRUCT BIOL. 1993 Jul 1;111(1):48-58. https://doi.org/10.1006/jsbi.1993.1035

Bibtex

@article{1484ac628c7b489f8cff89edfb1ba253,
title = "Cytoskeleton architecture of C6 rat glioma cell subclones differing in intermediate filament protein expression",
abstract = "Whole-mount electron microscopy was used in conjunction with immunogold labeling to characterize the cytoskeleton architecture of C6 rat glioma cell subclones. These subclones differ in intermediate filament (IF) protein composition and either contain vimentin (subclone C6D8) or do not express any of the known cytoplasmic IF proteins (subclone C6D10) (R{\"o}ser et al., 1991). In C6D8 cells short thin (3 nm) connecting filaments frequently linked vimentin to actin filaments and, in addition, connected vimentin filaments to each other. Occasionally, direct contacts were noticed between actin and vimentin filaments. Thin connecting filaments were present at a significantly higher number in IF-deficient C6D10 cells, forming a dense cytoplasmic network in conjunction with actin filament bundles as the dominating structure. The data indicate that thin connecting filaments are present in C6 cells independent of the expression of cytoplasmic IF proteins. They suggest that structural linkages between vimentin and actin filaments mediated by thin connecting filaments could play a major role in determining the cytoskeleton architecture of these cells.",
keywords = "Actins, Animals, Antibodies, Antibodies, Monoclonal, Clone Cells, Cytoskeleton, Glioma, Intermediate Filament Proteins, Microscopy, Electron, Microscopy, Immunoelectron, Microtubules, Rats, Tumor Cells, Cultured, Vimentin, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't",
author = "W Bohn and K R{\"o}ser and H Hohenberg and K Mannweiler and P Traub",
year = "1993",
month = jul,
day = "1",
doi = "10.1006/jsbi.1993.1035",
language = "English",
volume = "111",
pages = "48--58",
journal = "J STRUCT BIOL",
issn = "1047-8477",
publisher = "Academic Press Inc.",
number = "1",

}

RIS

TY - JOUR

T1 - Cytoskeleton architecture of C6 rat glioma cell subclones differing in intermediate filament protein expression

AU - Bohn, W

AU - Röser, K

AU - Hohenberg, H

AU - Mannweiler, K

AU - Traub, P

PY - 1993/7/1

Y1 - 1993/7/1

N2 - Whole-mount electron microscopy was used in conjunction with immunogold labeling to characterize the cytoskeleton architecture of C6 rat glioma cell subclones. These subclones differ in intermediate filament (IF) protein composition and either contain vimentin (subclone C6D8) or do not express any of the known cytoplasmic IF proteins (subclone C6D10) (Röser et al., 1991). In C6D8 cells short thin (3 nm) connecting filaments frequently linked vimentin to actin filaments and, in addition, connected vimentin filaments to each other. Occasionally, direct contacts were noticed between actin and vimentin filaments. Thin connecting filaments were present at a significantly higher number in IF-deficient C6D10 cells, forming a dense cytoplasmic network in conjunction with actin filament bundles as the dominating structure. The data indicate that thin connecting filaments are present in C6 cells independent of the expression of cytoplasmic IF proteins. They suggest that structural linkages between vimentin and actin filaments mediated by thin connecting filaments could play a major role in determining the cytoskeleton architecture of these cells.

AB - Whole-mount electron microscopy was used in conjunction with immunogold labeling to characterize the cytoskeleton architecture of C6 rat glioma cell subclones. These subclones differ in intermediate filament (IF) protein composition and either contain vimentin (subclone C6D8) or do not express any of the known cytoplasmic IF proteins (subclone C6D10) (Röser et al., 1991). In C6D8 cells short thin (3 nm) connecting filaments frequently linked vimentin to actin filaments and, in addition, connected vimentin filaments to each other. Occasionally, direct contacts were noticed between actin and vimentin filaments. Thin connecting filaments were present at a significantly higher number in IF-deficient C6D10 cells, forming a dense cytoplasmic network in conjunction with actin filament bundles as the dominating structure. The data indicate that thin connecting filaments are present in C6 cells independent of the expression of cytoplasmic IF proteins. They suggest that structural linkages between vimentin and actin filaments mediated by thin connecting filaments could play a major role in determining the cytoskeleton architecture of these cells.

KW - Actins

KW - Animals

KW - Antibodies

KW - Antibodies, Monoclonal

KW - Clone Cells

KW - Cytoskeleton

KW - Glioma

KW - Intermediate Filament Proteins

KW - Microscopy, Electron

KW - Microscopy, Immunoelectron

KW - Microtubules

KW - Rats

KW - Tumor Cells, Cultured

KW - Vimentin

KW - Comparative Study

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1006/jsbi.1993.1035

DO - 10.1006/jsbi.1993.1035

M3 - SCORING: Journal article

C2 - 8251263

VL - 111

SP - 48

EP - 58

JO - J STRUCT BIOL

JF - J STRUCT BIOL

SN - 1047-8477

IS - 1

ER -