Cultivation of keratinocytes and fibroblasts in a three-dimensional bovine collagen-elastin matrix (Matriderm®) and application for full thickness wound coverage in vivo
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Cultivation of keratinocytes and fibroblasts in a three-dimensional bovine collagen-elastin matrix (Matriderm®) and application for full thickness wound coverage in vivo. / Killat, Jasper; Reimers, Kerstin; Choi, Claudia Y; Jahn, Sabrina; Vogt, Peter M; Radtke, Christine.
in: INT J MOL SCI, Jahrgang 14, Nr. 7, 11.07.2013, S. 14460-74.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Cultivation of keratinocytes and fibroblasts in a three-dimensional bovine collagen-elastin matrix (Matriderm®) and application for full thickness wound coverage in vivo
AU - Killat, Jasper
AU - Reimers, Kerstin
AU - Choi, Claudia Y
AU - Jahn, Sabrina
AU - Vogt, Peter M
AU - Radtke, Christine
PY - 2013/7/11
Y1 - 2013/7/11
N2 - New skin substitutes for burn medicine or reconstructive surgery pose an important issue in plastic surgery. Matriderm® is a clinically approved three-dimensional bovine collagen-elastin matrix which is already used as a dermal substitute of full thickness burn wounds. The drawback of an avital matrix is the limited integration in full thickness skin defects, depending on the defect size. To further optimize this process, Matriderm® has also been studied as a matrix for tissue engineering of skin albeit long-term cultivation of the matrix with cells has been difficult. Cells have generally been seeded onto the matrix with high cell loss and minimal time-consuming migration. Here we developed a cell seeded skin equivalent after microtransfer of cells directly into the matrix. First, cells were cultured, and microinjected into Matriderm®. Then, cell viability in the matrix was determined by histology in vitro. As a next step, the skin substitute was applied in vivo into a full thickness rodent wound model. The wound coverage and healing was observed over a period of two weeks followed by histological examination assessing cell viability, proliferation and integration into the host. Viable and proliferating cells could be found throughout the entire matrix. The presented skin substitute resembles healthy skin in morphology and integrity. Based on this study, future investigations are planned to examine behaviour of epidermal stem cells injected into a collagen-elastin matrix under the aspects of establishment of stem cell niches and differentiation.
AB - New skin substitutes for burn medicine or reconstructive surgery pose an important issue in plastic surgery. Matriderm® is a clinically approved three-dimensional bovine collagen-elastin matrix which is already used as a dermal substitute of full thickness burn wounds. The drawback of an avital matrix is the limited integration in full thickness skin defects, depending on the defect size. To further optimize this process, Matriderm® has also been studied as a matrix for tissue engineering of skin albeit long-term cultivation of the matrix with cells has been difficult. Cells have generally been seeded onto the matrix with high cell loss and minimal time-consuming migration. Here we developed a cell seeded skin equivalent after microtransfer of cells directly into the matrix. First, cells were cultured, and microinjected into Matriderm®. Then, cell viability in the matrix was determined by histology in vitro. As a next step, the skin substitute was applied in vivo into a full thickness rodent wound model. The wound coverage and healing was observed over a period of two weeks followed by histological examination assessing cell viability, proliferation and integration into the host. Viable and proliferating cells could be found throughout the entire matrix. The presented skin substitute resembles healthy skin in morphology and integrity. Based on this study, future investigations are planned to examine behaviour of epidermal stem cells injected into a collagen-elastin matrix under the aspects of establishment of stem cell niches and differentiation.
KW - Animals
KW - Cattle
KW - Cell Culture Techniques
KW - Cell Lineage
KW - Collagen
KW - Elastin
KW - Fibroblasts
KW - Keratinocytes
KW - Mice
KW - NIH 3T3 Cells
KW - Skin
KW - Skin, Artificial
KW - Tissue Engineering
KW - Wound Healing
KW - Hautsubstitut
KW - Elastin-Kollagen Matrix
KW - Matriderm(R)
KW - Epidermale Stammzellen
KW - Fibroblasten
KW - Keratinozyten
U2 - 10.3390/ijms140714460
DO - 10.3390/ijms140714460
M3 - SCORING: Journal article
C2 - 23852021
VL - 14
SP - 14460
EP - 14474
JO - INT J MOL SCI
JF - INT J MOL SCI
SN - 1661-6596
IS - 7
ER -