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Crystallization and preliminary X-ray analysis of leukemia inhibitory factor.

Standard

Crystallization and preliminary X-ray analysis of leukemia inhibitory factor. / Betzel, C; Visanji, M; Dauter, Z; Fourme, R; Weber, Wolfgang; Marnitz, U; Boone, T; Pope, J; Miller, J; Hawkins, N.

in: FEBS LETT, Jahrgang 336, Nr. 2, 2, 1993, S. 236-238.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Betzel, C, Visanji, M, Dauter, Z, Fourme, R, Weber, W, Marnitz, U, Boone, T, Pope, J, Miller, J & Hawkins, N 1993, 'Crystallization and preliminary X-ray analysis of leukemia inhibitory factor.', FEBS LETT, Jg. 336, Nr. 2, 2, S. 236-238. <http://www.ncbi.nlm.nih.gov/pubmed/8262236?dopt=Citation>

APA

Betzel, C., Visanji, M., Dauter, Z., Fourme, R., Weber, W., Marnitz, U., Boone, T., Pope, J., Miller, J., & Hawkins, N. (1993). Crystallization and preliminary X-ray analysis of leukemia inhibitory factor. FEBS LETT, 336(2), 236-238. [2]. http://www.ncbi.nlm.nih.gov/pubmed/8262236?dopt=Citation

Vancouver

Betzel C, Visanji M, Dauter Z, Fourme R, Weber W, Marnitz U et al. Crystallization and preliminary X-ray analysis of leukemia inhibitory factor. FEBS LETT. 1993;336(2):236-238. 2.

Bibtex

@article{c433c1fbc9ae4f86bd3fd07facdc7c3f,
title = "Crystallization and preliminary X-ray analysis of leukemia inhibitory factor.",
abstract = "Leukemia inhibitory factor (LIF) is a polyfunctional molecule with significant and diverse biological activities. LIF is a glycoprotein secreted by a number of different cell types in vitro. It is induced in fibroblasts, lymphocytes, monocytes and astrocytes by various inducers such as serum, TNF, interleukin-IP and EGF. Due to extensive and variable glycosylation the molecular weight can range from 38 to 67 kDA. The biological functions of LIF are mediated through a receptor and a signal transducer, gp130, which is also used by factors like interleukin-6 (IL-6), cilliary neurotropic factor (CNTF), and oncostatin M (OSM). Here, we report the crystallization of the non-glycosylated human-like LIF expressed in E. coli. The present crystals diffract to 2.0 A using synchrotron radiation. They belong to the monoclinic space group C2, and the cell dimensions are a = 61.5 A, b = 45.3 A, c = 77.7 A and beta = 112.3 degrees.",
keywords = "Humans, Electrophoresis, Polyacrylamide Gel, Cloning, Molecular, Crystallography, X-Ray, Escherichia coli, Growth Inhibitors/*chemistry/genetics, *Interleukin-6, Isoelectric Focusing, Leukemia Inhibitory Factor, Lymphokines/*chemistry/genetics, Recombinant Proteins/chemistry/genetics, Humans, Electrophoresis, Polyacrylamide Gel, Cloning, Molecular, Crystallography, X-Ray, Escherichia coli, Growth Inhibitors/*chemistry/genetics, *Interleukin-6, Isoelectric Focusing, Leukemia Inhibitory Factor, Lymphokines/*chemistry/genetics, Recombinant Proteins/chemistry/genetics",
author = "C Betzel and M Visanji and Z Dauter and R Fourme and Wolfgang Weber and U Marnitz and T Boone and J Pope and J Miller and N Hawkins",
year = "1993",
language = "English",
volume = "336",
pages = "236--238",
journal = "FEBS LETT",
issn = "0014-5793",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Crystallization and preliminary X-ray analysis of leukemia inhibitory factor.

AU - Betzel, C

AU - Visanji, M

AU - Dauter, Z

AU - Fourme, R

AU - Weber, Wolfgang

AU - Marnitz, U

AU - Boone, T

AU - Pope, J

AU - Miller, J

AU - Hawkins, N

PY - 1993

Y1 - 1993

N2 - Leukemia inhibitory factor (LIF) is a polyfunctional molecule with significant and diverse biological activities. LIF is a glycoprotein secreted by a number of different cell types in vitro. It is induced in fibroblasts, lymphocytes, monocytes and astrocytes by various inducers such as serum, TNF, interleukin-IP and EGF. Due to extensive and variable glycosylation the molecular weight can range from 38 to 67 kDA. The biological functions of LIF are mediated through a receptor and a signal transducer, gp130, which is also used by factors like interleukin-6 (IL-6), cilliary neurotropic factor (CNTF), and oncostatin M (OSM). Here, we report the crystallization of the non-glycosylated human-like LIF expressed in E. coli. The present crystals diffract to 2.0 A using synchrotron radiation. They belong to the monoclinic space group C2, and the cell dimensions are a = 61.5 A, b = 45.3 A, c = 77.7 A and beta = 112.3 degrees.

AB - Leukemia inhibitory factor (LIF) is a polyfunctional molecule with significant and diverse biological activities. LIF is a glycoprotein secreted by a number of different cell types in vitro. It is induced in fibroblasts, lymphocytes, monocytes and astrocytes by various inducers such as serum, TNF, interleukin-IP and EGF. Due to extensive and variable glycosylation the molecular weight can range from 38 to 67 kDA. The biological functions of LIF are mediated through a receptor and a signal transducer, gp130, which is also used by factors like interleukin-6 (IL-6), cilliary neurotropic factor (CNTF), and oncostatin M (OSM). Here, we report the crystallization of the non-glycosylated human-like LIF expressed in E. coli. The present crystals diffract to 2.0 A using synchrotron radiation. They belong to the monoclinic space group C2, and the cell dimensions are a = 61.5 A, b = 45.3 A, c = 77.7 A and beta = 112.3 degrees.

KW - Humans

KW - Electrophoresis, Polyacrylamide Gel

KW - Cloning, Molecular

KW - Crystallography, X-Ray

KW - Escherichia coli

KW - Growth Inhibitors/chemistry/genetics

KW - Interleukin-6

KW - Isoelectric Focusing

KW - Leukemia Inhibitory Factor

KW - Lymphokines/chemistry/genetics

KW - Recombinant Proteins/chemistry/genetics

KW - Humans

KW - Electrophoresis, Polyacrylamide Gel

KW - Cloning, Molecular

KW - Crystallography, X-Ray

KW - Escherichia coli

KW - Growth Inhibitors/chemistry/genetics

KW - Interleukin-6

KW - Isoelectric Focusing

KW - Leukemia Inhibitory Factor

KW - Lymphokines/chemistry/genetics

KW - Recombinant Proteins/chemistry/genetics

M3 - SCORING: Journal article

VL - 336

SP - 236

EP - 238

JO - FEBS LETT

JF - FEBS LETT

SN - 0014-5793

IS - 2

M1 - 2

ER -