CRP1, a protein localized in filopodia of growth cones, is involved in dendritic growth.

Liping Ma; Jeffrey A Greenwood; Melitta Schachner

CRP1, a protein localized in filopodia of growth cones, is involved in dendritic growth.

Standard

CRP1, a protein localized in filopodia of growth cones, is involved in dendritic growth. / Ma, Liping; Greenwood, Jeffrey A; Schachner, Melitta.

in: J NEUROSCI, Jahrgang 31, Nr. 46, 46, 2011, S. 16781-16791.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Ma, L, Greenwood, JA & Schachner, M 2011, 'CRP1, a protein localized in filopodia of growth cones, is involved in dendritic growth.', J NEUROSCI, Jg. 31, Nr. 46, 46, S. 16781-16791. <http://www.ncbi.nlm.nih.gov/pubmed/22090504?dopt=Citation>

APA

Ma, L., Greenwood, J. A., & Schachner, M. (2011). CRP1, a protein localized in filopodia of growth cones, is involved in dendritic growth. J NEUROSCI, 31(46), 16781-16791. [46]. http://www.ncbi.nlm.nih.gov/pubmed/22090504?dopt=Citation

Vancouver

Ma L, Greenwood JA, Schachner M. CRP1, a protein localized in filopodia of growth cones, is involved in dendritic growth. J NEUROSCI. 2011;31(46):16781-16791. 46.

Bibtex

@article{647f298b978040bd8fc0bffe73e58d45,

title = "CRP1, a protein localized in filopodia of growth cones, is involved in dendritic growth.",

abstract = "The cysteine-rich protein (CRP) family is a subgroup of LIM domain proteins. CRP1, which cross-links actin filaments to make actin bundles, is the only CRP family member expressed in the CNS with little known about its function in nerve cells. Here, we report that CRP1 colocalizes with actin in the filopodia of growth cones in cultured rat hippocampal neurons. Knockdown of CRP1 expression by short hairpin RNA interference results in inhibition of filopodia formation and dendritic growth in neurons. Overexpression of CRP1 increases filopodia formation and neurite branching, which require its actin-bundling activity. Expression of CRP1 with a constitutively active form of Cdc42, a GTPase involved in filopodia formation, increases filopodia formation in COS-7 cells, suggesting cooperation between the two proteins. Moreover, we demonstrate that neuronal activity upregulates CRP1 expression in hippocampal neurons via Ca²? influx after depolarization. Ca²?/calmodulin-dependent protein kinase IV (CaMKIV) and cAMP response element binding protein mediate the Ca²?-induced upregulation of CRP1 expression. Furthermore, CRP1 is required for the dendritic growth induced by Ca²? influx or CaMKIV. Together, these data are the first to demonstrate a role for CRP1 in dendritic growth.",

keywords = "Animals, Male, Female, Cells, Cultured, Up-Regulation, Cercopithecus aethiops, Rats, Analysis of Variance, Microscopy, Confocal, Embryo, Mammalian, Hippocampus/cytology, Calcium/metabolism, RNA, Messenger/metabolism, RNA, Small Interfering/genetics/metabolism, Actins/metabolism, Dendrites/*physiology, Enzyme Inhibitors/pharmacology, Calcium Channel Blockers/pharmacology, Transfection/methods, 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine/analogs & derivatives/pharmacology, CREB-Binding Protein/metabolism, Calcium-Calmodulin-Dependent Protein Kinase Type 2/metabolism, Chelating Agents/pharmacology, Cystatins/genetics/*metabolism, Egtazic Acid/pharmacology, Green Fluorescent Proteins/genetics, Growth Cones/metabolism/*ultrastructure, Neurites/metabolism/*ultrastructure, Neurons/*cytology/drug effects, Nifedipine/pharmacology, Potassium Chloride/pharmacology, Pseudopodia/*metabolism, Valine/analogs & derivatives/pharmacology, Animals, Male, Female, Cells, Cultured, Up-Regulation, Cercopithecus aethiops, Rats, Analysis of Variance, Microscopy, Confocal, Embryo, Mammalian, Hippocampus/cytology, Calcium/metabolism, RNA, Messenger/metabolism, RNA, Small Interfering/genetics/metabolism, Actins/metabolism, Dendrites/*physiology, Enzyme Inhibitors/pharmacology, Calcium Channel Blockers/pharmacology, Transfection/methods, 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine/analogs & derivatives/pharmacology, CREB-Binding Protein/metabolism, Calcium-Calmodulin-Dependent Protein Kinase Type 2/metabolism, Chelating Agents/pharmacology, Cystatins/genetics/*metabolism, Egtazic Acid/pharmacology, Green Fluorescent Proteins/genetics, Growth Cones/metabolism/*ultrastructure, Neurites/metabolism/*ultrastructure, Neurons/*cytology/drug effects, Nifedipine/pharmacology, Potassium Chloride/pharmacology, Pseudopodia/*metabolism, Valine/analogs & derivatives/pharmacology",

author = "Liping Ma and Greenwood, {Jeffrey A} and Melitta Schachner",

year = "2011",

language = "English",

volume = "31",

pages = "16781--16791",

journal = "J NEUROSCI",

issn = "0270-6474",

publisher = "Society for Neuroscience",

number = "46",

}

RIS

TY - JOUR

T1 - CRP1, a protein localized in filopodia of growth cones, is involved in dendritic growth.

AU - Ma, Liping

AU - Greenwood, Jeffrey A

AU - Schachner, Melitta

PY - 2011

Y1 - 2011

N2 - The cysteine-rich protein (CRP) family is a subgroup of LIM domain proteins. CRP1, which cross-links actin filaments to make actin bundles, is the only CRP family member expressed in the CNS with little known about its function in nerve cells. Here, we report that CRP1 colocalizes with actin in the filopodia of growth cones in cultured rat hippocampal neurons. Knockdown of CRP1 expression by short hairpin RNA interference results in inhibition of filopodia formation and dendritic growth in neurons. Overexpression of CRP1 increases filopodia formation and neurite branching, which require its actin-bundling activity. Expression of CRP1 with a constitutively active form of Cdc42, a GTPase involved in filopodia formation, increases filopodia formation in COS-7 cells, suggesting cooperation between the two proteins. Moreover, we demonstrate that neuronal activity upregulates CRP1 expression in hippocampal neurons via Ca²? influx after depolarization. Ca²?/calmodulin-dependent protein kinase IV (CaMKIV) and cAMP response element binding protein mediate the Ca²?-induced upregulation of CRP1 expression. Furthermore, CRP1 is required for the dendritic growth induced by Ca²? influx or CaMKIV. Together, these data are the first to demonstrate a role for CRP1 in dendritic growth.

AB - The cysteine-rich protein (CRP) family is a subgroup of LIM domain proteins. CRP1, which cross-links actin filaments to make actin bundles, is the only CRP family member expressed in the CNS with little known about its function in nerve cells. Here, we report that CRP1 colocalizes with actin in the filopodia of growth cones in cultured rat hippocampal neurons. Knockdown of CRP1 expression by short hairpin RNA interference results in inhibition of filopodia formation and dendritic growth in neurons. Overexpression of CRP1 increases filopodia formation and neurite branching, which require its actin-bundling activity. Expression of CRP1 with a constitutively active form of Cdc42, a GTPase involved in filopodia formation, increases filopodia formation in COS-7 cells, suggesting cooperation between the two proteins. Moreover, we demonstrate that neuronal activity upregulates CRP1 expression in hippocampal neurons via Ca²? influx after depolarization. Ca²?/calmodulin-dependent protein kinase IV (CaMKIV) and cAMP response element binding protein mediate the Ca²?-induced upregulation of CRP1 expression. Furthermore, CRP1 is required for the dendritic growth induced by Ca²? influx or CaMKIV. Together, these data are the first to demonstrate a role for CRP1 in dendritic growth.

KW - Animals

KW - Male

KW - Female

KW - Cells, Cultured

KW - Up-Regulation

KW - Cercopithecus aethiops

KW - Rats

KW - Analysis of Variance

KW - Microscopy, Confocal

KW - Embryo, Mammalian

KW - Hippocampus/cytology

KW - Calcium/metabolism

KW - RNA, Messenger/metabolism

KW - RNA, Small Interfering/genetics/metabolism

KW - Actins/metabolism

KW - Dendrites/physiology

KW - Enzyme Inhibitors/pharmacology

KW - Calcium Channel Blockers/pharmacology

KW - Transfection/methods

KW - 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine/analogs & derivatives/pharmacology

KW - CREB-Binding Protein/metabolism

KW - Calcium-Calmodulin-Dependent Protein Kinase Type 2/metabolism

KW - Chelating Agents/pharmacology

KW - Cystatins/genetics/metabolism

KW - Egtazic Acid/pharmacology

KW - Green Fluorescent Proteins/genetics

KW - Growth Cones/metabolism/ultrastructure

KW - Neurites/metabolism/ultrastructure

KW - Neurons/cytology/drug effects

KW - Nifedipine/pharmacology

KW - Potassium Chloride/pharmacology

KW - Pseudopodia/metabolism

KW - Valine/analogs & derivatives/pharmacology