Concordance between MITS and conventional autopsies for pathological and virological diagnoses
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Concordance between MITS and conventional autopsies for pathological and virological diagnoses. / Schädler, Julia; Azeke, Akhator Terence; Ondruschka, Benjamin; Steurer, Stefan; Lütgehetmann, Marc; Fitzek, Antonia; Möbius, Dustin.
in: INT J LEGAL MED, Jahrgang 138, Nr. 2, 03.2024, S. 431-442.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Concordance between MITS and conventional autopsies for pathological and virological diagnoses
AU - Schädler, Julia
AU - Azeke, Akhator Terence
AU - Ondruschka, Benjamin
AU - Steurer, Stefan
AU - Lütgehetmann, Marc
AU - Fitzek, Antonia
AU - Möbius, Dustin
N1 - © 2023. The Author(s).
PY - 2024/3
Y1 - 2024/3
N2 - In pandemics or to further study highly contagious infectious diseases, new strategies are needed for the collection of post-mortem tissue samples to identify the pathogen as well as its morphological impact. In this study, an ultrasound-guided minimally invasive tissue sampling (MITS) protocol was developed and validated for post-mortem use. The histological and microbiological qualities of post-mortem specimens were evaluated and compared between MITS and conventional autopsy (CA) in a series of COVID-19 deaths. Thirty-six ultrasound-guided MITS were performed. In five cases more, specimens for histological and virological examination were also obtained and compared during the subsequently performed CA. Summary statistics and qualitative interpretations (positive, negative) were calculated for each organ tissue sample from MITS and CA, and target genes were determined for both human cell count (beta-globin) and virus (SARS-CoV-2 specific E gene). There are no significant differences between MITS and CA with respect to the detectability of viral load in individual organs, which is why MITS can be of utmost importance and an useful alternative, especially during outbreaks of infectious diseases.
AB - In pandemics or to further study highly contagious infectious diseases, new strategies are needed for the collection of post-mortem tissue samples to identify the pathogen as well as its morphological impact. In this study, an ultrasound-guided minimally invasive tissue sampling (MITS) protocol was developed and validated for post-mortem use. The histological and microbiological qualities of post-mortem specimens were evaluated and compared between MITS and conventional autopsy (CA) in a series of COVID-19 deaths. Thirty-six ultrasound-guided MITS were performed. In five cases more, specimens for histological and virological examination were also obtained and compared during the subsequently performed CA. Summary statistics and qualitative interpretations (positive, negative) were calculated for each organ tissue sample from MITS and CA, and target genes were determined for both human cell count (beta-globin) and virus (SARS-CoV-2 specific E gene). There are no significant differences between MITS and CA with respect to the detectability of viral load in individual organs, which is why MITS can be of utmost importance and an useful alternative, especially during outbreaks of infectious diseases.
U2 - 10.1007/s00414-023-03088-w
DO - 10.1007/s00414-023-03088-w
M3 - SCORING: Journal article
C2 - 37837537
VL - 138
SP - 431
EP - 442
JO - INT J LEGAL MED
JF - INT J LEGAL MED
SN - 0937-9827
IS - 2
ER -
