Completely functional double-barreled chloride channel expressed from a single Torpedo cDNA.
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Completely functional double-barreled chloride channel expressed from a single Torpedo cDNA. / Bauer, Christiane K.; Steinmeyer, K; Schwarz, J R; Jentsch, T J.
in: P NATL ACAD SCI USA, Jahrgang 88, Nr. 24, 1991, S. 11052-11056.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - Completely functional double-barreled chloride channel expressed from a single Torpedo cDNA.
AU - Bauer, Christiane K.
AU - Steinmeyer, K
AU - Schwarz, J R
AU - Jentsch, T J
PY - 1991
Y1 - 1991
N2 - We have performed an electrophysiological analysis of the recently cloned Torpedo marmorata Cl- channel. Functional expression of Cl- channels in oocytes of Xenopus laevis previously injected with cRNA yielded an outward-rectifying current activated by hyperpolarization. Replacement of Cl- with other anions significantly reduced or inhibited the current. Single-channel recordings from cell-attached patches exhibited burst-like Cl- channel activity with rapid fluctuations between three equally spaced substates (0 pS, 9 pS, and 18 pS). The properties of the cloned Cl- channel were almost identical to those of the reconstituted native T. californica Cl- channel and were in full agreement with the predictions of the double-barreled channel model [Hanke, W. ; Miller, C. (1983) J. Gen. Physiol. 82, 25-45]. Our results imply that the cloned cDNA codes for the completely functional Torpedo electroplax Cl- channel.
AB - We have performed an electrophysiological analysis of the recently cloned Torpedo marmorata Cl- channel. Functional expression of Cl- channels in oocytes of Xenopus laevis previously injected with cRNA yielded an outward-rectifying current activated by hyperpolarization. Replacement of Cl- with other anions significantly reduced or inhibited the current. Single-channel recordings from cell-attached patches exhibited burst-like Cl- channel activity with rapid fluctuations between three equally spaced substates (0 pS, 9 pS, and 18 pS). The properties of the cloned Cl- channel were almost identical to those of the reconstituted native T. californica Cl- channel and were in full agreement with the predictions of the double-barreled channel model [Hanke, W. ; Miller, C. (1983) J. Gen. Physiol. 82, 25-45]. Our results imply that the cloned cDNA codes for the completely functional Torpedo electroplax Cl- channel.
U2 - 10.1073/pnas.88.24.11052
DO - 10.1073/pnas.88.24.11052
M3 - SCORING: Zeitschriftenaufsatz
VL - 88
SP - 11052
EP - 11056
JO - P NATL ACAD SCI USA
JF - P NATL ACAD SCI USA
SN - 0027-8424
IS - 24
ER -
