Comparing individual NK cell activity in vitro.

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Comparing individual NK cell activity in vitro. / Sierich, Heidi; Eiermann, Thomas.

in: Curr Protoc Immunol, Jahrgang Chapter 14, 2013.

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@article{7225d3f3eb2948c392176338fb3630d7,
title = "Comparing individual NK cell activity in vitro.",
abstract = "Natural killer (NK) cells play an important role in the innate immune system by eliminating infected and mutated cells. Their cytotoxic capacities vary markedly among individuals. The cytotoxic activity can be measured in peripheral blood mononuclear cells (PBMCs) using the NK cell-specific target cell line K562. In this chapter, we present a protocol for the standardization and normalization of cell preparation and NK cell cytotoxicity measurement in a (51)Cr-release assay. By following these protocols, it is possible to compare the NK cell activity of numerous-if necessary selected-individuals in vitro.",
author = "Heidi Sierich and Thomas Eiermann",
year = "2013",
language = "English",
volume = "Chapter 14",
journal = "Curr Protoc Immunol",
issn = "1934-3671",
publisher = "John Wiley and Sons Inc.",

}

RIS

TY - JOUR

T1 - Comparing individual NK cell activity in vitro.

AU - Sierich, Heidi

AU - Eiermann, Thomas

PY - 2013

Y1 - 2013

N2 - Natural killer (NK) cells play an important role in the innate immune system by eliminating infected and mutated cells. Their cytotoxic capacities vary markedly among individuals. The cytotoxic activity can be measured in peripheral blood mononuclear cells (PBMCs) using the NK cell-specific target cell line K562. In this chapter, we present a protocol for the standardization and normalization of cell preparation and NK cell cytotoxicity measurement in a (51)Cr-release assay. By following these protocols, it is possible to compare the NK cell activity of numerous-if necessary selected-individuals in vitro.

AB - Natural killer (NK) cells play an important role in the innate immune system by eliminating infected and mutated cells. Their cytotoxic capacities vary markedly among individuals. The cytotoxic activity can be measured in peripheral blood mononuclear cells (PBMCs) using the NK cell-specific target cell line K562. In this chapter, we present a protocol for the standardization and normalization of cell preparation and NK cell cytotoxicity measurement in a (51)Cr-release assay. By following these protocols, it is possible to compare the NK cell activity of numerous-if necessary selected-individuals in vitro.

M3 - SCORING: Journal article

VL - Chapter 14

JO - Curr Protoc Immunol

JF - Curr Protoc Immunol

SN - 1934-3671

ER -