Comparative expressed sequence hybridization detects recurrent patterns of altered sequence expression in oral squamous cell carcinoma
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Comparative expressed sequence hybridization detects recurrent patterns of altered sequence expression in oral squamous cell carcinoma. / Janjetovic, Snjezana; Sticht, Carsten; Knoepfle, Karl; Joos, Stefan; Hofele, Christof; Lichter, Peter; Freier, Kolja.
in: Oncology reports, Jahrgang 24, Nr. 2, 01.08.2010, S. 369-74.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Comparative expressed sequence hybridization detects recurrent patterns of altered sequence expression in oral squamous cell carcinoma
AU - Janjetovic, Snjezana
AU - Sticht, Carsten
AU - Knoepfle, Karl
AU - Joos, Stefan
AU - Hofele, Christof
AU - Lichter, Peter
AU - Freier, Kolja
PY - 2010/8/1
Y1 - 2010/8/1
N2 - Despite its common histology and presentation, oral squamous cell carcinoma (OSCC) is associated with widely varying clinical behaviour and response to therapy. To further elucidate the molecular basis of OSCC, an approach for gene expression analysis termed comparative expressed sequence hybridization (CESH) was used in the present study. This straightforward approach allows the rapid delineation of pathophysiologically interesting candidate chromosome regions by a direct detection of aberrant transcriptional activation. CESH profiling of OSCC specimens led to the identification of several novel chromosomal regions. Increased expression compared to a set of control mucosa specimens was found on 1q22-q23, 3q26.3-qter, 4q31.1-q32, 11q12-q13.2, 14q32, 18q12, 19q13.2-q13.3 and 22q13.1-q13.2. Decreased expression was found on 8p22-p23, 16p12 and 16q23-q24. Using CESH, common patterns of altered sequence expression in different OSCC samples were obtained. While some of these regions overlap with those known to be frequently altered in OSCC on the genomic level, this screen revealed novel chromosome subregions with increased transcriptional activity, which are probably independent of the genomic status of the tumor cells.
AB - Despite its common histology and presentation, oral squamous cell carcinoma (OSCC) is associated with widely varying clinical behaviour and response to therapy. To further elucidate the molecular basis of OSCC, an approach for gene expression analysis termed comparative expressed sequence hybridization (CESH) was used in the present study. This straightforward approach allows the rapid delineation of pathophysiologically interesting candidate chromosome regions by a direct detection of aberrant transcriptional activation. CESH profiling of OSCC specimens led to the identification of several novel chromosomal regions. Increased expression compared to a set of control mucosa specimens was found on 1q22-q23, 3q26.3-qter, 4q31.1-q32, 11q12-q13.2, 14q32, 18q12, 19q13.2-q13.3 and 22q13.1-q13.2. Decreased expression was found on 8p22-p23, 16p12 and 16q23-q24. Using CESH, common patterns of altered sequence expression in different OSCC samples were obtained. While some of these regions overlap with those known to be frequently altered in OSCC on the genomic level, this screen revealed novel chromosome subregions with increased transcriptional activity, which are probably independent of the genomic status of the tumor cells.
KW - Carcinoma, Squamous Cell
KW - Case-Control Studies
KW - Chromosome Aberrations
KW - Chromosomes, Human
KW - Comparative Genomic Hybridization
KW - Gene Dosage
KW - Gene Expression Profiling
KW - Gene Expression Regulation, Neoplastic
KW - Humans
KW - Mouth Neoplasms
KW - Oligonucleotide Array Sequence Analysis
M3 - SCORING: Journal article
C2 - 20596623
VL - 24
SP - 369
EP - 374
JO - ONCOL REP
JF - ONCOL REP
SN - 1021-335X
IS - 2
ER -