Comparative 3D micro-CT and 2D histomorphometry analysis of dental implant osseointegration in the maxilla of minipigs
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Comparative 3D micro-CT and 2D histomorphometry analysis of dental implant osseointegration in the maxilla of minipigs. / Bissinger, Oliver; Probst, Florian Andreas; Wolff, Klaus-Dietrich; Jeschke, Anke; Weitz, Jochen; Deppe, Herbert; Kolk, Andreas.
in: J CLIN PERIODONTOL, Jahrgang 44, Nr. 4, 04.2017, S. 418-427.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Comparative 3D micro-CT and 2D histomorphometry analysis of dental implant osseointegration in the maxilla of minipigs
AU - Bissinger, Oliver
AU - Probst, Florian Andreas
AU - Wolff, Klaus-Dietrich
AU - Jeschke, Anke
AU - Weitz, Jochen
AU - Deppe, Herbert
AU - Kolk, Andreas
N1 - This article is protected by copyright. All rights reserved.
PY - 2017/4
Y1 - 2017/4
N2 - AIM: The bone implant contact (BIC) has traditionally been evaluated with histological methods. Thereupon, strong correlations of 2-dimensional (2D) BIC have been detected between μCT and destructive histology. However, due to the high intra-sample variability of BIC values, one histological slice is not sufficient to represent 3D BIC. Therefore, our aim has been to correlate the averaged values of 3-4 histological sections to 3D μCT.MATERIAL AND METHODS: Fifty four implants inserted into the maxilla of 14 minipigs were evaluated. Two different time points were selected to assess the 3D BIC (distance to implant: 2-5 voxels), an inner ring (6-30 voxels) and an outer ring (55-100 voxels) by using μCT (voxel size: 10 μm) and to correlate the values to histomorphometry.RESULTS: Strong correlations (p<0.0001; 28d, 56d, total) were seen between μCT and histomorphometry concerning BIC (r=0.84, r=0.85, r=0.83), the inner ring (r=0.87, r=0.87, r=0.88) and the outer ring (r=0.85, r=0.85, r=0.88). Closer to the implant, μ-CT values were higher compared with histomorphometry.CONCLUSION: Although 3-4 histological slices per implant seem to predict the 3D BIC, μCT might be advantageous because of its nondestructive 3D character. The healing time may not impact on the comparability. This article is protected by copyright. All rights reserved.
AB - AIM: The bone implant contact (BIC) has traditionally been evaluated with histological methods. Thereupon, strong correlations of 2-dimensional (2D) BIC have been detected between μCT and destructive histology. However, due to the high intra-sample variability of BIC values, one histological slice is not sufficient to represent 3D BIC. Therefore, our aim has been to correlate the averaged values of 3-4 histological sections to 3D μCT.MATERIAL AND METHODS: Fifty four implants inserted into the maxilla of 14 minipigs were evaluated. Two different time points were selected to assess the 3D BIC (distance to implant: 2-5 voxels), an inner ring (6-30 voxels) and an outer ring (55-100 voxels) by using μCT (voxel size: 10 μm) and to correlate the values to histomorphometry.RESULTS: Strong correlations (p<0.0001; 28d, 56d, total) were seen between μCT and histomorphometry concerning BIC (r=0.84, r=0.85, r=0.83), the inner ring (r=0.87, r=0.87, r=0.88) and the outer ring (r=0.85, r=0.85, r=0.88). Closer to the implant, μ-CT values were higher compared with histomorphometry.CONCLUSION: Although 3-4 histological slices per implant seem to predict the 3D BIC, μCT might be advantageous because of its nondestructive 3D character. The healing time may not impact on the comparability. This article is protected by copyright. All rights reserved.
U2 - 10.1111/jcpe.12693
DO - 10.1111/jcpe.12693
M3 - SCORING: Journal article
C2 - 28063250
VL - 44
SP - 418
EP - 427
JO - J CLIN PERIODONTOL
JF - J CLIN PERIODONTOL
SN - 0303-6979
IS - 4
ER -
