Collagen type I hydrogel allows migration, proliferation, and osteogenic differentiation of rat bone marrow stromal cells.
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Collagen type I hydrogel allows migration, proliferation, and osteogenic differentiation of rat bone marrow stromal cells. / Hesse, Eric; Hefferan, Theresa E; Tarara, James E; Haasper, Carl; Meller, Rupert; Krettek, Christian; Lu, Lichun; Yaszemski, Michael J.
in: J BIOMED MATER RES A, Jahrgang 94, Nr. 2, 2, 2010, S. 442-449.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Collagen type I hydrogel allows migration, proliferation, and osteogenic differentiation of rat bone marrow stromal cells.
AU - Hesse, Eric
AU - Hefferan, Theresa E
AU - Tarara, James E
AU - Haasper, Carl
AU - Meller, Rupert
AU - Krettek, Christian
AU - Lu, Lichun
AU - Yaszemski, Michael J
PY - 2010
Y1 - 2010
N2 - Hydrogels are potentially useful for many purposes in regenerative medicine including drug and growth factor delivery, as single scaffold for bone repair or as a filler of pores of another biomaterial in which host mesenchymal progenitor cells can migrate in and differentiate into matrix-producing osteoblasts. Collagen type I is of special interest as it is a very important and abundant natural matrix component. The purpose of this study was to investigate whether rat bone marrow stromal cells (rBMSCs) are able to adhere to, to survive, to proliferate and to migrate in collagen type I hydrogels and whether they can adopt an osteoblastic fate. rBMSCs were obtained from rat femora and plated on collagen type I hydrogels. Before harvest by day 7, 14, and 21, hydrogels were fluorescently labeled, cryo-cut and analyzed by fluorescent-based and laser scanning confocal microscopy to determine cell proliferation, migration, and viability. Osteogenic differentiation was determined by alkaline phosphatase activity. Collagen type I hydrogels allowed the attachment of rBMSCs to the hydrogel, their proliferation, and migration towards the inner part of the gel. rBMSCs started to differentiate into osteoblasts as determined by an increase in alkaline phosphatase activity after two weeks in culture. This study therefore suggests that collagen type I hydrogels could be useful for musculoskeletal regenerative therapies.
AB - Hydrogels are potentially useful for many purposes in regenerative medicine including drug and growth factor delivery, as single scaffold for bone repair or as a filler of pores of another biomaterial in which host mesenchymal progenitor cells can migrate in and differentiate into matrix-producing osteoblasts. Collagen type I is of special interest as it is a very important and abundant natural matrix component. The purpose of this study was to investigate whether rat bone marrow stromal cells (rBMSCs) are able to adhere to, to survive, to proliferate and to migrate in collagen type I hydrogels and whether they can adopt an osteoblastic fate. rBMSCs were obtained from rat femora and plated on collagen type I hydrogels. Before harvest by day 7, 14, and 21, hydrogels were fluorescently labeled, cryo-cut and analyzed by fluorescent-based and laser scanning confocal microscopy to determine cell proliferation, migration, and viability. Osteogenic differentiation was determined by alkaline phosphatase activity. Collagen type I hydrogels allowed the attachment of rBMSCs to the hydrogel, their proliferation, and migration towards the inner part of the gel. rBMSCs started to differentiate into osteoblasts as determined by an increase in alkaline phosphatase activity after two weeks in culture. This study therefore suggests that collagen type I hydrogels could be useful for musculoskeletal regenerative therapies.
KW - Animals
KW - Male
KW - Cells, Cultured
KW - Rats
KW - Rats, Sprague-Dawley
KW - Cattle
KW - Materials Testing
KW - Osteoblasts/cytology/metabolism
KW - Osteogenesis/physiology
KW - Biocompatible Materials/metabolism
KW - Bone Marrow Cells/cytology/physiology
KW - Cell Differentiation/physiology
KW - Cell Movement/physiology
KW - Cell Proliferation
KW - Collagen Type I/metabolism
KW - Hydrogel/metabolism
KW - Animals
KW - Male
KW - Cells, Cultured
KW - Rats
KW - Rats, Sprague-Dawley
KW - Cattle
KW - Materials Testing
KW - Osteoblasts/cytology/metabolism
KW - Osteogenesis/physiology
KW - Biocompatible Materials/metabolism
KW - Bone Marrow Cells/cytology/physiology
KW - Cell Differentiation/physiology
KW - Cell Movement/physiology
KW - Cell Proliferation
KW - Collagen Type I/metabolism
KW - Hydrogel/metabolism
M3 - SCORING: Journal article
VL - 94
SP - 442
EP - 449
JO - J BIOMED MATER RES A
JF - J BIOMED MATER RES A
SN - 1549-3296
IS - 2
M1 - 2
ER -
