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Clonal expansion and HPV-induced immortalization are early molecular alterations in cervical carcinogenesis.

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Clonal expansion and HPV-induced immortalization are early molecular alterations in cervical carcinogenesis. / Park, Tjoung-Won; Riethdorf, Sabine; Schulz, Gudrun; Riethdorf, Lutz; Wright, Thomas; Löning, Thomas.

in: ANTICANCER RES, Jahrgang 23, Nr. 1, 1, 2003, S. 155-160.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Park, T-W, Riethdorf, S, Schulz, G, Riethdorf, L, Wright, T & Löning, T 2003, 'Clonal expansion and HPV-induced immortalization are early molecular alterations in cervical carcinogenesis.', ANTICANCER RES, Jg. 23, Nr. 1, 1, S. 155-160. <http://www.ncbi.nlm.nih.gov/pubmed/12680207?dopt=Citation>

APA

Park, T-W., Riethdorf, S., Schulz, G., Riethdorf, L., Wright, T., & Löning, T. (2003). Clonal expansion and HPV-induced immortalization are early molecular alterations in cervical carcinogenesis. ANTICANCER RES, 23(1), 155-160. [1]. http://www.ncbi.nlm.nih.gov/pubmed/12680207?dopt=Citation

Vancouver

Park T-W, Riethdorf S, Schulz G, Riethdorf L, Wright T, Löning T. Clonal expansion and HPV-induced immortalization are early molecular alterations in cervical carcinogenesis. ANTICANCER RES. 2003;23(1):155-160. 1.

Bibtex

@article{dc9410e5e5b84f669eed214c0813305b,
title = "Clonal expansion and HPV-induced immortalization are early molecular alterations in cervical carcinogenesis.",
abstract = "BACKGROUND: Monoclonality, a hallmark of most neoplasias, is found in high-grade squamous intraepithelial lesions (Hi-SIL) and some low-grade SILs (Lo-SIL). The transforming genes E6/E7 of HPV 16 have been shown to induce telomerase activity and immortalization. We investigated the role of immortalization in monoclonal and polyclonal SILs. MATERIALS AND METHODS: Telomerase RNA (hTR) and HPV 16 E6/E7 were investigated in 45 Lo-, 33 Hi-SILs and 11 cervical carcinomas (SCC) by RNA/RNA in situ hybridization. Clonality in this series has been described previously. RESULTS: Expression of hTR and viral oncogenes correlated significantly with the histological severity of the lesion (p <0.001). Intense focal up-regulation of hTR was found in 14 out of 22 monoclonal Hi-SILs, 4 out of 20 monoclonal Lo-SILs but only 1 out of 15 polyclonal Lo-SIL. HPV 16 E6/E7 expression was detected in 20 out of 22 monoclonal Hi-SILs but only in 5 out of 21 monoclonal Lo-SILs. CONCLUSION: Monoclonal expansion and immortalization are early alterations predominantly found in SCC and Hi-SILs, but also in a subset of Lo-SILs.",
author = "Tjoung-Won Park and Sabine Riethdorf and Gudrun Schulz and Lutz Riethdorf and Thomas Wright and Thomas L{\"o}ning",
year = "2003",
language = "Deutsch",
volume = "23",
pages = "155--160",
journal = "ANTICANCER RES",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
number = "1",

}

RIS

TY - JOUR

T1 - Clonal expansion and HPV-induced immortalization are early molecular alterations in cervical carcinogenesis.

AU - Park, Tjoung-Won

AU - Riethdorf, Sabine

AU - Schulz, Gudrun

AU - Riethdorf, Lutz

AU - Wright, Thomas

AU - Löning, Thomas

PY - 2003

Y1 - 2003

N2 - BACKGROUND: Monoclonality, a hallmark of most neoplasias, is found in high-grade squamous intraepithelial lesions (Hi-SIL) and some low-grade SILs (Lo-SIL). The transforming genes E6/E7 of HPV 16 have been shown to induce telomerase activity and immortalization. We investigated the role of immortalization in monoclonal and polyclonal SILs. MATERIALS AND METHODS: Telomerase RNA (hTR) and HPV 16 E6/E7 were investigated in 45 Lo-, 33 Hi-SILs and 11 cervical carcinomas (SCC) by RNA/RNA in situ hybridization. Clonality in this series has been described previously. RESULTS: Expression of hTR and viral oncogenes correlated significantly with the histological severity of the lesion (p <0.001). Intense focal up-regulation of hTR was found in 14 out of 22 monoclonal Hi-SILs, 4 out of 20 monoclonal Lo-SILs but only 1 out of 15 polyclonal Lo-SIL. HPV 16 E6/E7 expression was detected in 20 out of 22 monoclonal Hi-SILs but only in 5 out of 21 monoclonal Lo-SILs. CONCLUSION: Monoclonal expansion and immortalization are early alterations predominantly found in SCC and Hi-SILs, but also in a subset of Lo-SILs.

AB - BACKGROUND: Monoclonality, a hallmark of most neoplasias, is found in high-grade squamous intraepithelial lesions (Hi-SIL) and some low-grade SILs (Lo-SIL). The transforming genes E6/E7 of HPV 16 have been shown to induce telomerase activity and immortalization. We investigated the role of immortalization in monoclonal and polyclonal SILs. MATERIALS AND METHODS: Telomerase RNA (hTR) and HPV 16 E6/E7 were investigated in 45 Lo-, 33 Hi-SILs and 11 cervical carcinomas (SCC) by RNA/RNA in situ hybridization. Clonality in this series has been described previously. RESULTS: Expression of hTR and viral oncogenes correlated significantly with the histological severity of the lesion (p <0.001). Intense focal up-regulation of hTR was found in 14 out of 22 monoclonal Hi-SILs, 4 out of 20 monoclonal Lo-SILs but only 1 out of 15 polyclonal Lo-SIL. HPV 16 E6/E7 expression was detected in 20 out of 22 monoclonal Hi-SILs but only in 5 out of 21 monoclonal Lo-SILs. CONCLUSION: Monoclonal expansion and immortalization are early alterations predominantly found in SCC and Hi-SILs, but also in a subset of Lo-SILs.

M3 - SCORING: Zeitschriftenaufsatz

VL - 23

SP - 155

EP - 160

JO - ANTICANCER RES

JF - ANTICANCER RES

SN - 0250-7005

IS - 1

M1 - 1

ER -