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Clonal dominance of hematopoietic stem cells triggered by retroviral gene marking

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Clonal dominance of hematopoietic stem cells triggered by retroviral gene marking. / Kustikova, Olga; Fehse, Boris; Modlich, Ute; Yang, Min; Düllmann, Jochen; Kamino, Kenji; von Neuhoff, Nils; Schlegelberger, Brigitte; Li, Zhixiong; Baum, Christopher.

in: SCIENCE, Jahrgang 308, Nr. 5725, 20.05.2005, S. 1171-4.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Kustikova, O, Fehse, B, Modlich, U, Yang, M, Düllmann, J, Kamino, K, von Neuhoff, N, Schlegelberger, B, Li, Z & Baum, C 2005, 'Clonal dominance of hematopoietic stem cells triggered by retroviral gene marking', SCIENCE, Jg. 308, Nr. 5725, S. 1171-4. https://doi.org/10.1126/science.1105063

APA

Kustikova, O., Fehse, B., Modlich, U., Yang, M., Düllmann, J., Kamino, K., von Neuhoff, N., Schlegelberger, B., Li, Z., & Baum, C. (2005). Clonal dominance of hematopoietic stem cells triggered by retroviral gene marking. SCIENCE, 308(5725), 1171-4. https://doi.org/10.1126/science.1105063

Vancouver

Kustikova O, Fehse B, Modlich U, Yang M, Düllmann J, Kamino K et al. Clonal dominance of hematopoietic stem cells triggered by retroviral gene marking. SCIENCE. 2005 Mai 20;308(5725):1171-4. https://doi.org/10.1126/science.1105063

Bibtex

@article{a7a6de0709654464b2810e542c08e3b8,
title = "Clonal dominance of hematopoietic stem cells triggered by retroviral gene marking",
abstract = "Gene marking with replication-defective retroviral vectors has been used for more than 20 years to track the in vivo fate of cell clones. We demonstrate that retroviral integrations themselves may trigger nonmalignant clonal expansion in murine long-term hematopoiesis. All 29 insertions recovered from clones dominating in serially transplanted recipients affected loci with an established or potential role in the self-renewal or survival of hematopoietic stem cells. Transcriptional dysregulation occurred in all 12 insertion sites analyzed. These findings have major implications for diagnostic gene marking and the discovery of genes regulating stem cell turnover.",
keywords = "Animals, Antigens, CD34/genetics, Bone Marrow Transplantation, DNA-Binding Proteins/genetics, Down-Regulation, Genetic Vectors, Hematopoiesis, Hematopoietic Stem Cell Transplantation, Hematopoietic Stem Cells/physiology, Humans, Ligase Chain Reaction, MDS1 and EVI1 Complex Locus Protein, Mice, Mice, Inbred C57BL, Mutagenesis, Insertional, Polymerase Chain Reaction, Proto-Oncogenes/genetics, Retroviridae/genetics, Transcription Factors/genetics, Transcription, Genetic, Transgenes, Up-Regulation, Virus Integration",
author = "Olga Kustikova and Boris Fehse and Ute Modlich and Min Yang and Jochen D{\"u}llmann and Kenji Kamino and {von Neuhoff}, Nils and Brigitte Schlegelberger and Zhixiong Li and Christopher Baum",
year = "2005",
month = may,
day = "20",
doi = "10.1126/science.1105063",
language = "English",
volume = "308",
pages = "1171--4",
journal = "SCIENCE",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "5725",

}

RIS

TY - JOUR

T1 - Clonal dominance of hematopoietic stem cells triggered by retroviral gene marking

AU - Kustikova, Olga

AU - Fehse, Boris

AU - Modlich, Ute

AU - Yang, Min

AU - Düllmann, Jochen

AU - Kamino, Kenji

AU - von Neuhoff, Nils

AU - Schlegelberger, Brigitte

AU - Li, Zhixiong

AU - Baum, Christopher

PY - 2005/5/20

Y1 - 2005/5/20

N2 - Gene marking with replication-defective retroviral vectors has been used for more than 20 years to track the in vivo fate of cell clones. We demonstrate that retroviral integrations themselves may trigger nonmalignant clonal expansion in murine long-term hematopoiesis. All 29 insertions recovered from clones dominating in serially transplanted recipients affected loci with an established or potential role in the self-renewal or survival of hematopoietic stem cells. Transcriptional dysregulation occurred in all 12 insertion sites analyzed. These findings have major implications for diagnostic gene marking and the discovery of genes regulating stem cell turnover.

AB - Gene marking with replication-defective retroviral vectors has been used for more than 20 years to track the in vivo fate of cell clones. We demonstrate that retroviral integrations themselves may trigger nonmalignant clonal expansion in murine long-term hematopoiesis. All 29 insertions recovered from clones dominating in serially transplanted recipients affected loci with an established or potential role in the self-renewal or survival of hematopoietic stem cells. Transcriptional dysregulation occurred in all 12 insertion sites analyzed. These findings have major implications for diagnostic gene marking and the discovery of genes regulating stem cell turnover.

KW - Animals

KW - Antigens, CD34/genetics

KW - Bone Marrow Transplantation

KW - DNA-Binding Proteins/genetics

KW - Down-Regulation

KW - Genetic Vectors

KW - Hematopoiesis

KW - Hematopoietic Stem Cell Transplantation

KW - Hematopoietic Stem Cells/physiology

KW - Humans

KW - Ligase Chain Reaction

KW - MDS1 and EVI1 Complex Locus Protein

KW - Mice

KW - Mice, Inbred C57BL

KW - Mutagenesis, Insertional

KW - Polymerase Chain Reaction

KW - Proto-Oncogenes/genetics

KW - Retroviridae/genetics

KW - Transcription Factors/genetics

KW - Transcription, Genetic

KW - Transgenes

KW - Up-Regulation

KW - Virus Integration

U2 - 10.1126/science.1105063

DO - 10.1126/science.1105063

M3 - SCORING: Journal article

C2 - 15905401

VL - 308

SP - 1171

EP - 1174

JO - SCIENCE

JF - SCIENCE

SN - 0036-8075

IS - 5725

ER -