Circulating tumour cells escape from EpCAM-based detection due to epithelial-to-mesenchymal transition.
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Circulating tumour cells escape from EpCAM-based detection due to epithelial-to-mesenchymal transition. / Gorges, Tobias; Tinhofer, Ingeborg; Drosch, Michael; Röse, Lars; Zollner, Thomas M; Krahn, Thomas; von Ahsen, Oliver.
in: BMC CANCER, Jahrgang 12, 2012, S. 178.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Circulating tumour cells escape from EpCAM-based detection due to epithelial-to-mesenchymal transition.
AU - Gorges, Tobias
AU - Tinhofer, Ingeborg
AU - Drosch, Michael
AU - Röse, Lars
AU - Zollner, Thomas M
AU - Krahn, Thomas
AU - von Ahsen, Oliver
PY - 2012
Y1 - 2012
N2 - Circulating tumour cells (CTCs) have shown prognostic relevance in metastatic breast, prostate, colon and pancreatic cancer. For further development of CTCs as a biomarker, we compared the performance of different protocols for CTC detection in murine breast cancer xenograft models (MDA-MB-231, MDA-MB-468 and KPL-4). Blood samples were taken from tumour bearing animals (20 to 200?mm2) and analysed for CTCs using 1. an epithelial marker based enrichment method (AdnaTest), 2. an antibody independent technique, targeting human gene transcripts (qualitative PCR), and 3. an antibody-independent approach, targeting human DNA-sequences (quantitative PCR). Further, gene expression changes associated with epithelial-to-mesenchymal transition (EMT) were determined with an EMT-specific PCR assay.
AB - Circulating tumour cells (CTCs) have shown prognostic relevance in metastatic breast, prostate, colon and pancreatic cancer. For further development of CTCs as a biomarker, we compared the performance of different protocols for CTC detection in murine breast cancer xenograft models (MDA-MB-231, MDA-MB-468 and KPL-4). Blood samples were taken from tumour bearing animals (20 to 200?mm2) and analysed for CTCs using 1. an epithelial marker based enrichment method (AdnaTest), 2. an antibody independent technique, targeting human gene transcripts (qualitative PCR), and 3. an antibody-independent approach, targeting human DNA-sequences (quantitative PCR). Further, gene expression changes associated with epithelial-to-mesenchymal transition (EMT) were determined with an EMT-specific PCR assay.
KW - Animals
KW - Humans
KW - Female
KW - Mice
KW - Cell Line, Tumor
KW - Neoplasm Metastasis
KW - Mice, Nude
KW - Transplantation, Heterologous
KW - Epithelial-Mesenchymal Transition
KW - Cell Separation/methods
KW - Antigens, Neoplasm/analysis/blood
KW - Breast Neoplasms/blood/pathology
KW - Cell Adhesion Molecules/analysis/blood
KW - Neoplastic Cells, Circulating/chemistry/metabolism/pathology
KW - Tumor Markers, Biological/analysis/blood
KW - Animals
KW - Humans
KW - Female
KW - Mice
KW - Cell Line, Tumor
KW - Neoplasm Metastasis
KW - Mice, Nude
KW - Transplantation, Heterologous
KW - Epithelial-Mesenchymal Transition
KW - Cell Separation/methods
KW - Antigens, Neoplasm/analysis/blood
KW - Breast Neoplasms/blood/pathology
KW - Cell Adhesion Molecules/analysis/blood
KW - Neoplastic Cells, Circulating/chemistry/metabolism/pathology
KW - Tumor Markers, Biological/analysis/blood
M3 - SCORING: Journal article
VL - 12
SP - 178
JO - BMC CANCER
JF - BMC CANCER
SN - 1471-2407
ER -
