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Chronic granulomatous disease (CGD) and complete myeloperoxidase deficiency both yield strongly reduced dihydrorhodamine 123 test signals but can be easily discerned in routine testing for CGD

Standard

Chronic granulomatous disease (CGD) and complete myeloperoxidase deficiency both yield strongly reduced dihydrorhodamine 123 test signals but can be easily discerned in routine testing for CGD. / Mauch, Lysann; Lun, Andreas; O'Gorman, Maurice R G; Harris, John S; Schulze, Ilka; Zychlinsky, Arturo; Fuchs, Tobias; Oelschlägel, Uta; Brenner, Sebastian; Kutter, Dolphe; Rösen-Wolff, Angela; Roesler, Joachim.

in: CLIN CHEM, Jahrgang 53, Nr. 5, 01.05.2007, S. 890-6.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Mauch, L, Lun, A, O'Gorman, MRG, Harris, JS, Schulze, I, Zychlinsky, A, Fuchs, T, Oelschlägel, U, Brenner, S, Kutter, D, Rösen-Wolff, A & Roesler, J 2007, 'Chronic granulomatous disease (CGD) and complete myeloperoxidase deficiency both yield strongly reduced dihydrorhodamine 123 test signals but can be easily discerned in routine testing for CGD', CLIN CHEM, Jg. 53, Nr. 5, S. 890-6. https://doi.org/10.1373/clinchem.2006.083444

APA

Mauch, L., Lun, A., O'Gorman, M. R. G., Harris, J. S., Schulze, I., Zychlinsky, A., Fuchs, T., Oelschlägel, U., Brenner, S., Kutter, D., Rösen-Wolff, A., & Roesler, J. (2007). Chronic granulomatous disease (CGD) and complete myeloperoxidase deficiency both yield strongly reduced dihydrorhodamine 123 test signals but can be easily discerned in routine testing for CGD. CLIN CHEM, 53(5), 890-6. https://doi.org/10.1373/clinchem.2006.083444

Vancouver

Mauch L, Lun A, O'Gorman MRG, Harris JS, Schulze I, Zychlinsky A et al. Chronic granulomatous disease (CGD) and complete myeloperoxidase deficiency both yield strongly reduced dihydrorhodamine 123 test signals but can be easily discerned in routine testing for CGD. CLIN CHEM. 2007 Mai 1;53(5):890-6. https://doi.org/10.1373/clinchem.2006.083444

Bibtex

@article{edb3c9ca23b841ffaa36b7d94065a7d6,
title = "Chronic granulomatous disease (CGD) and complete myeloperoxidase deficiency both yield strongly reduced dihydrorhodamine 123 test signals but can be easily discerned in routine testing for CGD",
abstract = "BACKGROUND: The flow cytometric dihydrorhodamine 123 (DHR) assay is used as a screening test for chronic granulomatous disease (CGD), but complete myeloperoxidase (MPO) deficiency can also lead to a strongly decreased DHR signal. Our aim was to devise simple laboratory methods to differentiate MPO deficiency (false positive for CGD) and NADPH oxidase abnormalities (true CGD).METHODS: We measured NADPH-oxidase and MPO activity in neutrophils from MPO-deficient patients, CGD patients, NADPH-oxidase-transfected K562 cells and cells with inhibited and substituted MPO.RESULTS: Eosinophils from MPO-deficient individuals retain eosinophilic peroxidase and therefore generate a normal DHR signal. The addition of recombinant human MPO enhances the DHR signal when simply added to a suspension of MPO-deficient cells but not when added to NADPH-oxidase-deficient (CGD) cells. Lucigenin-enhanced chemiluminescence (LCL) is increased in neutrophils from MPO-deficient patients, whereas neutrophils from patients with CGD show a decreased response.CONCLUSIONS: A false-positive result caused by MPO deficiency can be easily ascertained because, unlike cells from a CGD patient, cells from MPO-deficient patients (a) contain functionally normal eosinophils, (b) show a significant enhancement of the DHR signal following addition of rhMPO, and (c) generate a strong LCL signal.",
keywords = "Acridines, Coloring Agents, Diagnosis, Differential, Eosinophils, False Positive Reactions, Female, Flow Cytometry, Granulomatous Disease, Chronic, Humans, K562 Cells, Luminescent Agents, Luminescent Measurements, Male, NADPH Oxidase, Neutrophils, Peroxidase, Recombinant Proteins, Rhodamines, Transfection",
author = "Lysann Mauch and Andreas Lun and O'Gorman, {Maurice R G} and Harris, {John S} and Ilka Schulze and Arturo Zychlinsky and Tobias Fuchs and Uta Oelschl{\"a}gel and Sebastian Brenner and Dolphe Kutter and Angela R{\"o}sen-Wolff and Joachim Roesler",
year = "2007",
month = may,
day = "1",
doi = "10.1373/clinchem.2006.083444",
language = "English",
volume = "53",
pages = "890--6",
journal = "CLIN CHEM",
issn = "0009-9147",
publisher = "American Association for Clinical Chemistry Inc.",
number = "5",

}

RIS

TY - JOUR

T1 - Chronic granulomatous disease (CGD) and complete myeloperoxidase deficiency both yield strongly reduced dihydrorhodamine 123 test signals but can be easily discerned in routine testing for CGD

AU - Mauch, Lysann

AU - Lun, Andreas

AU - O'Gorman, Maurice R G

AU - Harris, John S

AU - Schulze, Ilka

AU - Zychlinsky, Arturo

AU - Fuchs, Tobias

AU - Oelschlägel, Uta

AU - Brenner, Sebastian

AU - Kutter, Dolphe

AU - Rösen-Wolff, Angela

AU - Roesler, Joachim

PY - 2007/5/1

Y1 - 2007/5/1

N2 - BACKGROUND: The flow cytometric dihydrorhodamine 123 (DHR) assay is used as a screening test for chronic granulomatous disease (CGD), but complete myeloperoxidase (MPO) deficiency can also lead to a strongly decreased DHR signal. Our aim was to devise simple laboratory methods to differentiate MPO deficiency (false positive for CGD) and NADPH oxidase abnormalities (true CGD).METHODS: We measured NADPH-oxidase and MPO activity in neutrophils from MPO-deficient patients, CGD patients, NADPH-oxidase-transfected K562 cells and cells with inhibited and substituted MPO.RESULTS: Eosinophils from MPO-deficient individuals retain eosinophilic peroxidase and therefore generate a normal DHR signal. The addition of recombinant human MPO enhances the DHR signal when simply added to a suspension of MPO-deficient cells but not when added to NADPH-oxidase-deficient (CGD) cells. Lucigenin-enhanced chemiluminescence (LCL) is increased in neutrophils from MPO-deficient patients, whereas neutrophils from patients with CGD show a decreased response.CONCLUSIONS: A false-positive result caused by MPO deficiency can be easily ascertained because, unlike cells from a CGD patient, cells from MPO-deficient patients (a) contain functionally normal eosinophils, (b) show a significant enhancement of the DHR signal following addition of rhMPO, and (c) generate a strong LCL signal.

AB - BACKGROUND: The flow cytometric dihydrorhodamine 123 (DHR) assay is used as a screening test for chronic granulomatous disease (CGD), but complete myeloperoxidase (MPO) deficiency can also lead to a strongly decreased DHR signal. Our aim was to devise simple laboratory methods to differentiate MPO deficiency (false positive for CGD) and NADPH oxidase abnormalities (true CGD).METHODS: We measured NADPH-oxidase and MPO activity in neutrophils from MPO-deficient patients, CGD patients, NADPH-oxidase-transfected K562 cells and cells with inhibited and substituted MPO.RESULTS: Eosinophils from MPO-deficient individuals retain eosinophilic peroxidase and therefore generate a normal DHR signal. The addition of recombinant human MPO enhances the DHR signal when simply added to a suspension of MPO-deficient cells but not when added to NADPH-oxidase-deficient (CGD) cells. Lucigenin-enhanced chemiluminescence (LCL) is increased in neutrophils from MPO-deficient patients, whereas neutrophils from patients with CGD show a decreased response.CONCLUSIONS: A false-positive result caused by MPO deficiency can be easily ascertained because, unlike cells from a CGD patient, cells from MPO-deficient patients (a) contain functionally normal eosinophils, (b) show a significant enhancement of the DHR signal following addition of rhMPO, and (c) generate a strong LCL signal.

KW - Acridines

KW - Coloring Agents

KW - Diagnosis, Differential

KW - Eosinophils

KW - False Positive Reactions

KW - Female

KW - Flow Cytometry

KW - Granulomatous Disease, Chronic

KW - Humans

KW - K562 Cells

KW - Luminescent Agents

KW - Luminescent Measurements

KW - Male

KW - NADPH Oxidase

KW - Neutrophils

KW - Peroxidase

KW - Recombinant Proteins

KW - Rhodamines

KW - Transfection

U2 - 10.1373/clinchem.2006.083444

DO - 10.1373/clinchem.2006.083444

M3 - SCORING: Journal article

C2 - 17384005

VL - 53

SP - 890

EP - 896

JO - CLIN CHEM

JF - CLIN CHEM

SN - 0009-9147

IS - 5

ER -