Chronic equine hepacivirus infection in an adult gelding with severe hepatopathy
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Chronic equine hepacivirus infection in an adult gelding with severe hepatopathy. / Tegtmeyer, Birthe; Echelmeyer, Julia; Pfankuche, Vanessa M; Puff, Christina; Todt, Daniel; Fischer, Nicole; Durham, Andy; Feige, Karsten; Baumgärtner, Wolfgang; Steinmann, Eike; Cavalleri, Jessika-M V.
in: VET MED SCI, Jahrgang 5, Nr. 3, 08.2019, S. 372-378.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Chronic equine hepacivirus infection in an adult gelding with severe hepatopathy
AU - Tegtmeyer, Birthe
AU - Echelmeyer, Julia
AU - Pfankuche, Vanessa M
AU - Puff, Christina
AU - Todt, Daniel
AU - Fischer, Nicole
AU - Durham, Andy
AU - Feige, Karsten
AU - Baumgärtner, Wolfgang
AU - Steinmann, Eike
AU - Cavalleri, Jessika-M V
N1 - © 2019 The Authors. Veterinary Medicine and Science Published by John Wiley & Sons Ltd.
PY - 2019/8
Y1 - 2019/8
N2 - BACKGROUND: Equine hepacivirus (EqHV) in equids represents the closest homologue to hepatitis C virus (HCV) infecting humans. A majority of HCV infected patients develop a chronic course of infection leading to liver fibrosis, cirrhosis and liver failure. However, in horses mostly transient mild subclinical infections are reported for EqHV to date.OBJECTIVES: EqHV can be involved in chronic liver diseases of horses.METHODS: Biochemical parameters in serum samples were measured. Viral load was determined using qPCR. Next generation sequencing (NGS) of serum was performed. Liver tissue was stained with haematoxylin and eosin and analysed for viral RNA with fluorescent in situ-hybridization.RESULTS: The horse showed symptoms of severe hepatopathy and was chronically infected with EqHV. Viral RNA was detectable in the liver during disease. To rule out other infectious agents NGS was performed and showed the highest abundance for EqHV. The identified virus sequence was similar to other circulating equine hepaciviruses.CONCLUSIONS: EqHV can be associated with liver disease in horses. Whether it causes the disease or contributes in a multifactorial manner needs further investigation.
AB - BACKGROUND: Equine hepacivirus (EqHV) in equids represents the closest homologue to hepatitis C virus (HCV) infecting humans. A majority of HCV infected patients develop a chronic course of infection leading to liver fibrosis, cirrhosis and liver failure. However, in horses mostly transient mild subclinical infections are reported for EqHV to date.OBJECTIVES: EqHV can be involved in chronic liver diseases of horses.METHODS: Biochemical parameters in serum samples were measured. Viral load was determined using qPCR. Next generation sequencing (NGS) of serum was performed. Liver tissue was stained with haematoxylin and eosin and analysed for viral RNA with fluorescent in situ-hybridization.RESULTS: The horse showed symptoms of severe hepatopathy and was chronically infected with EqHV. Viral RNA was detectable in the liver during disease. To rule out other infectious agents NGS was performed and showed the highest abundance for EqHV. The identified virus sequence was similar to other circulating equine hepaciviruses.CONCLUSIONS: EqHV can be associated with liver disease in horses. Whether it causes the disease or contributes in a multifactorial manner needs further investigation.
KW - Animals
KW - Chronic Disease/veterinary
KW - Hepacivirus/isolation & purification
KW - Hepatitis C/diagnosis
KW - Horse Diseases/diagnosis
KW - Horses
KW - Liver Diseases/diagnosis
KW - Male
U2 - 10.1002/vms3.181
DO - 10.1002/vms3.181
M3 - SCORING: Journal article
C2 - 31267690
VL - 5
SP - 372
EP - 378
JO - VET MED SCI
JF - VET MED SCI
SN - 2053-1095
IS - 3
ER -
