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Chitosan encapsulation modulates the effect of capsaicin on the tight junctions of MDCK cells

Standard

Chitosan encapsulation modulates the effect of capsaicin on the tight junctions of MDCK cells. / Kaiser, M; Pereira, S; Pohl, L; Ketelhut, S; Kemper, B; Gorzelanny, C; Galla, H-J; Moerschbacher, B M; Goycoolea, F M.

in: SCI REP-UK, Jahrgang 5, 13.05.2015, S. 10048.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Kaiser, M, Pereira, S, Pohl, L, Ketelhut, S, Kemper, B, Gorzelanny, C, Galla, H-J, Moerschbacher, BM & Goycoolea, FM 2015, 'Chitosan encapsulation modulates the effect of capsaicin on the tight junctions of MDCK cells', SCI REP-UK, Jg. 5, S. 10048. https://doi.org/10.1038/srep10048

APA

Kaiser, M., Pereira, S., Pohl, L., Ketelhut, S., Kemper, B., Gorzelanny, C., Galla, H-J., Moerschbacher, B. M., & Goycoolea, F. M. (2015). Chitosan encapsulation modulates the effect of capsaicin on the tight junctions of MDCK cells. SCI REP-UK, 5, 10048. https://doi.org/10.1038/srep10048

Vancouver

Kaiser M, Pereira S, Pohl L, Ketelhut S, Kemper B, Gorzelanny C et al. Chitosan encapsulation modulates the effect of capsaicin on the tight junctions of MDCK cells. SCI REP-UK. 2015 Mai 13;5:10048. https://doi.org/10.1038/srep10048

Bibtex

@article{8b38346c237c4f4cb5dccabb1adb1261,
title = "Chitosan encapsulation modulates the effect of capsaicin on the tight junctions of MDCK cells",
abstract = "Capsaicin has known pharmacological effects including the ability to reversibly open cellular tight junctions, among others. The aim of this study was to develop a strategy to enhance the paracellular transport of a substance with low permeability (FITC-dextran) across an epithelial cell monolayer via reversible opening of cellular tight junctions using a nanosystem comprised by capsaicin and of chitosan. We compared the biophysical properties of free capsaicin and capsaicin-loaded chitosan nanocapsules, including their cytotoxicity towards epithelial MDCK-C7 cells and their effect on the integrity of tight junctions, membrane permeability and cellular uptake. The cytotoxic response of MDCK-C7 cells to capsaicin at a concentration of 500 μM, which was evident for the free compound, is not observable following its encapsulation. The interaction between nanocapsules and the tight junctions of MDCK-C7 cells was investigated by impedance spectroscopy, digital holographic microscopy and structured illumination fluorescence microscopy. The nanocapsules modulated the interaction between capsaicin and tight junctions as shown by the different time profile of trans-epithelial electrical resistance and the enhanced permeability of monolayers incubated with FITC-dextran. Structured illumination fluorescence microscopy showed that the nanocapsules were internalized by MDCK-C7 cells. The capsaicin-loaded nanocapsules could be further developed as drug nanocarriers with enhanced epithelial permeability.",
keywords = "Animals, Capsaicin, Capsules, Cell Line, Chemistry, Pharmaceutical, Chitosan, Drug Liberation, Emulsions, Nanotechnology, Permeability, Tight Junctions, Journal Article, Research Support, Non-U.S. Gov't",
author = "M Kaiser and S Pereira and L Pohl and S Ketelhut and B Kemper and C Gorzelanny and H-J Galla and Moerschbacher, {B M} and Goycoolea, {F M}",
year = "2015",
month = may,
day = "13",
doi = "10.1038/srep10048",
language = "English",
volume = "5",
pages = "10048",
journal = "SCI REP-UK",
issn = "2045-2322",
publisher = "NATURE PUBLISHING GROUP",

}

RIS

TY - JOUR

T1 - Chitosan encapsulation modulates the effect of capsaicin on the tight junctions of MDCK cells

AU - Kaiser, M

AU - Pereira, S

AU - Pohl, L

AU - Ketelhut, S

AU - Kemper, B

AU - Gorzelanny, C

AU - Galla, H-J

AU - Moerschbacher, B M

AU - Goycoolea, F M

PY - 2015/5/13

Y1 - 2015/5/13

N2 - Capsaicin has known pharmacological effects including the ability to reversibly open cellular tight junctions, among others. The aim of this study was to develop a strategy to enhance the paracellular transport of a substance with low permeability (FITC-dextran) across an epithelial cell monolayer via reversible opening of cellular tight junctions using a nanosystem comprised by capsaicin and of chitosan. We compared the biophysical properties of free capsaicin and capsaicin-loaded chitosan nanocapsules, including their cytotoxicity towards epithelial MDCK-C7 cells and their effect on the integrity of tight junctions, membrane permeability and cellular uptake. The cytotoxic response of MDCK-C7 cells to capsaicin at a concentration of 500 μM, which was evident for the free compound, is not observable following its encapsulation. The interaction between nanocapsules and the tight junctions of MDCK-C7 cells was investigated by impedance spectroscopy, digital holographic microscopy and structured illumination fluorescence microscopy. The nanocapsules modulated the interaction between capsaicin and tight junctions as shown by the different time profile of trans-epithelial electrical resistance and the enhanced permeability of monolayers incubated with FITC-dextran. Structured illumination fluorescence microscopy showed that the nanocapsules were internalized by MDCK-C7 cells. The capsaicin-loaded nanocapsules could be further developed as drug nanocarriers with enhanced epithelial permeability.

AB - Capsaicin has known pharmacological effects including the ability to reversibly open cellular tight junctions, among others. The aim of this study was to develop a strategy to enhance the paracellular transport of a substance with low permeability (FITC-dextran) across an epithelial cell monolayer via reversible opening of cellular tight junctions using a nanosystem comprised by capsaicin and of chitosan. We compared the biophysical properties of free capsaicin and capsaicin-loaded chitosan nanocapsules, including their cytotoxicity towards epithelial MDCK-C7 cells and their effect on the integrity of tight junctions, membrane permeability and cellular uptake. The cytotoxic response of MDCK-C7 cells to capsaicin at a concentration of 500 μM, which was evident for the free compound, is not observable following its encapsulation. The interaction between nanocapsules and the tight junctions of MDCK-C7 cells was investigated by impedance spectroscopy, digital holographic microscopy and structured illumination fluorescence microscopy. The nanocapsules modulated the interaction between capsaicin and tight junctions as shown by the different time profile of trans-epithelial electrical resistance and the enhanced permeability of monolayers incubated with FITC-dextran. Structured illumination fluorescence microscopy showed that the nanocapsules were internalized by MDCK-C7 cells. The capsaicin-loaded nanocapsules could be further developed as drug nanocarriers with enhanced epithelial permeability.

KW - Animals

KW - Capsaicin

KW - Capsules

KW - Cell Line

KW - Chemistry, Pharmaceutical

KW - Chitosan

KW - Drug Liberation

KW - Emulsions

KW - Nanotechnology

KW - Permeability

KW - Tight Junctions

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1038/srep10048

DO - 10.1038/srep10048

M3 - SCORING: Journal article

C2 - 25970096

VL - 5

SP - 10048

JO - SCI REP-UK

JF - SCI REP-UK

SN - 2045-2322

ER -