Chip-on-the-tip compact flexible endoscopic epifluorescence video-microscope for in-vivo imaging in medicine and biomedical research
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Chip-on-the-tip compact flexible endoscopic epifluorescence video-microscope for in-vivo imaging in medicine and biomedical research. / Matz, Gregor; Messerschmidt, Bernhard; Göbel, Werner; Filser, Severin; Betz, Christian S; Kirsch, Matthias; Uckermann, Ortrud; Kunze, Marcel; Flämig, Sven; Ehrhardt, André; Irion, Klaus-Martin; Haack, Mareike; Dorostkar, Mario M; Herms, Jochen; Gross, Herbert.
in: BIOMED OPT EXPRESS, Jahrgang 8, Nr. 7, 01.07.2017, S. 3329-3342.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Chip-on-the-tip compact flexible endoscopic epifluorescence video-microscope for in-vivo imaging in medicine and biomedical research
AU - Matz, Gregor
AU - Messerschmidt, Bernhard
AU - Göbel, Werner
AU - Filser, Severin
AU - Betz, Christian S
AU - Kirsch, Matthias
AU - Uckermann, Ortrud
AU - Kunze, Marcel
AU - Flämig, Sven
AU - Ehrhardt, André
AU - Irion, Klaus-Martin
AU - Haack, Mareike
AU - Dorostkar, Mario M
AU - Herms, Jochen
AU - Gross, Herbert
PY - 2017/7/1
Y1 - 2017/7/1
N2 - We demonstrate a 60 mg light video-endomicroscope with a cylindrical shape of the rigid tip of only 1.6 mm diameter and 6.7 mm length. A novel implementation method of the illumination unit in the endomicroscope is presented. It allows for the illumination of the biological sample with fiber-coupled LED light at 455 nm and the imaging of the red-shifted fluorescence light above 500 nm in epi-direction. A large numerical aperture of 0.7 leads to a sub-cellular resolution and yields to high-contrast images within a field of view of 160 μm. A miniaturized chip-on-the-tip CMOS image sensor with more than 150,000 pixels captures the multicolor images at 30 fps. Considering size, plug-and-play capability, optical performance, flexibility and weight, we hence present a probe which sets a new benchmark in the field of epifluorescence endomicroscopes. Several ex-vivo and in-vivo experiments in rodents and humans suggest future application in biomedical fields, especially in the neuroscience community, as well as in medical applications targeting optical biopsies or the detection of cellular anomalies.
AB - We demonstrate a 60 mg light video-endomicroscope with a cylindrical shape of the rigid tip of only 1.6 mm diameter and 6.7 mm length. A novel implementation method of the illumination unit in the endomicroscope is presented. It allows for the illumination of the biological sample with fiber-coupled LED light at 455 nm and the imaging of the red-shifted fluorescence light above 500 nm in epi-direction. A large numerical aperture of 0.7 leads to a sub-cellular resolution and yields to high-contrast images within a field of view of 160 μm. A miniaturized chip-on-the-tip CMOS image sensor with more than 150,000 pixels captures the multicolor images at 30 fps. Considering size, plug-and-play capability, optical performance, flexibility and weight, we hence present a probe which sets a new benchmark in the field of epifluorescence endomicroscopes. Several ex-vivo and in-vivo experiments in rodents and humans suggest future application in biomedical fields, especially in the neuroscience community, as well as in medical applications targeting optical biopsies or the detection of cellular anomalies.
KW - Journal Article
U2 - 10.1364/BOE.8.003329
DO - 10.1364/BOE.8.003329
M3 - SCORING: Journal article
C2 - 28717570
VL - 8
SP - 3329
EP - 3342
JO - BIOMED OPT EXPRESS
JF - BIOMED OPT EXPRESS
SN - 2156-7085
IS - 7
ER -
