Characterizing light-regulated retinal microRNAs reveals rapid turnover as a common property of neuronal microRNAs.
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Characterizing light-regulated retinal microRNAs reveals rapid turnover as a common property of neuronal microRNAs. / Krol, Jacek; Busskamp, Volker; Markiewicz, Ilona; Stadler, Michael B; Ribi, Sebastian; Richter, Jens; Duebel, Jens; Bicker, Silvia; Fehling, Hans Jörg; Schübeler, Dirk; Oertner, Thomas G.; Schratt, Gerhard; Bibel, Miriam; Roska, Botond; Filipowicz, Witold.
in: CELL, Jahrgang 141, Nr. 4, 4, 2010, S. 618-631.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Characterizing light-regulated retinal microRNAs reveals rapid turnover as a common property of neuronal microRNAs.
AU - Krol, Jacek
AU - Busskamp, Volker
AU - Markiewicz, Ilona
AU - Stadler, Michael B
AU - Ribi, Sebastian
AU - Richter, Jens
AU - Duebel, Jens
AU - Bicker, Silvia
AU - Fehling, Hans Jörg
AU - Schübeler, Dirk
AU - Oertner, Thomas G.
AU - Schratt, Gerhard
AU - Bibel, Miriam
AU - Roska, Botond
AU - Filipowicz, Witold
PY - 2010
Y1 - 2010
N2 - Adaptation to different levels of illumination is central to the function of the retina. Here, we demonstrate that levels of the miR-183/96/182 cluster, miR-204, and miR-211 are regulated by different light levels in the mouse retina. Concentrations of these microRNAs were downregulated during dark adaptation and upregulated in light-adapted retinas, with rapid decay and increased transcription being responsible for the respective changes. We identified the voltage-dependent glutamate transporter Slc1a1 as one of the miR-183/96/182 targets in photoreceptor cells. We found that microRNAs in retinal neurons decay much faster than microRNAs in nonneuronal cells. The high turnover is also characteristic of microRNAs in hippocampal and cortical neurons, and neurons differentiated from ES cells in vitro. Blocking activity reduced turnover of microRNAs in neuronal cells while stimulation with glutamate accelerated it. Our results demonstrate that microRNA metabolism in neurons is higher than in most other cells types and linked to neuronal activity.
AB - Adaptation to different levels of illumination is central to the function of the retina. Here, we demonstrate that levels of the miR-183/96/182 cluster, miR-204, and miR-211 are regulated by different light levels in the mouse retina. Concentrations of these microRNAs were downregulated during dark adaptation and upregulated in light-adapted retinas, with rapid decay and increased transcription being responsible for the respective changes. We identified the voltage-dependent glutamate transporter Slc1a1 as one of the miR-183/96/182 targets in photoreceptor cells. We found that microRNAs in retinal neurons decay much faster than microRNAs in nonneuronal cells. The high turnover is also characteristic of microRNAs in hippocampal and cortical neurons, and neurons differentiated from ES cells in vitro. Blocking activity reduced turnover of microRNAs in neuronal cells while stimulation with glutamate accelerated it. Our results demonstrate that microRNA metabolism in neurons is higher than in most other cells types and linked to neuronal activity.
KW - Animals
KW - Mice
KW - Up-Regulation
KW - Dark Adaptation
KW - Neurons/metabolism
KW - Down-Regulation
KW - Embryonic Stem Cells
KW - Excitatory Amino Acid Transporter 3/genetics/metabolism
KW - MicroRNAs/metabolism
KW - Photoreceptor Cells, Vertebrate/metabolism
KW - Retinal Neurons/metabolism
KW - Animals
KW - Mice
KW - Up-Regulation
KW - Dark Adaptation
KW - Neurons/metabolism
KW - Down-Regulation
KW - Embryonic Stem Cells
KW - Excitatory Amino Acid Transporter 3/genetics/metabolism
KW - MicroRNAs/metabolism
KW - Photoreceptor Cells, Vertebrate/metabolism
KW - Retinal Neurons/metabolism
M3 - SCORING: Journal article
VL - 141
SP - 618
EP - 631
JO - CELL
JF - CELL
SN - 0092-8674
IS - 4
M1 - 4
ER -
