Characterization of two human IgM monoclonal antibodies reactive with HLA-B27.
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Characterization of two human IgM monoclonal antibodies reactive with HLA-B27. / Wölpl, A; Toubert, A; Siemoneit, K; Eiermann, Thomas; Neumayer, H; Goldmann, S F.
in: TISSUE ANTIGENS, Jahrgang 46, Nr. 4, 4, 1995, S. 305-312.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Characterization of two human IgM monoclonal antibodies reactive with HLA-B27.
AU - Wölpl, A
AU - Toubert, A
AU - Siemoneit, K
AU - Eiermann, Thomas
AU - Neumayer, H
AU - Goldmann, S F
PY - 1995
Y1 - 1995
N2 - We describe here the generation and characterization of two human monoclonal IgM antibodies (UL-4F11 and UL-F6) reactive with HLA-B27. The monoclonal antibody (mAb) UL-4F11 is cytotoxic for peripheral mononuclear cells and, therefore, useful as typing reagent for HLA-B27 and HLA-B38. Protein chemistry showed that the mAb UL-4F11 precipitates HLA-B27 molecules. Epitope mapping analysis suggests that the amino acids 45, 67, 82 and 83 (alpha-1 domain) of the HLA-B27 sequence are necessary for mAb UL-4F11 reactivity. The mAb UL-F6 is suitable for complement dependent lysis of lymphoblastoid cell lines and stimulated peripheral blood mononuclear cells with HLA-B27 (B*2701, B*2702, B*2703, B*2705, B*2707), B13, B40 (60,61), B47 and B48 specificities. Its reactivity indicates that the amino acid valine in position 152 and glutamic acid in position 163 of the alpha-2 domain are crucial for the binding epitope.
AB - We describe here the generation and characterization of two human monoclonal IgM antibodies (UL-4F11 and UL-F6) reactive with HLA-B27. The monoclonal antibody (mAb) UL-4F11 is cytotoxic for peripheral mononuclear cells and, therefore, useful as typing reagent for HLA-B27 and HLA-B38. Protein chemistry showed that the mAb UL-4F11 precipitates HLA-B27 molecules. Epitope mapping analysis suggests that the amino acids 45, 67, 82 and 83 (alpha-1 domain) of the HLA-B27 sequence are necessary for mAb UL-4F11 reactivity. The mAb UL-F6 is suitable for complement dependent lysis of lymphoblastoid cell lines and stimulated peripheral blood mononuclear cells with HLA-B27 (B*2701, B*2702, B*2703, B*2705, B*2707), B13, B40 (60,61), B47 and B48 specificities. Its reactivity indicates that the amino acid valine in position 152 and glutamic acid in position 163 of the alpha-2 domain are crucial for the binding epitope.
M3 - SCORING: Zeitschriftenaufsatz
VL - 46
SP - 305
EP - 312
JO - TISSUE ANTIGENS
JF - TISSUE ANTIGENS
SN - 0001-2815
IS - 4
M1 - 4
ER -
