Characterization of eight different tetracyclines: advances in fluorescence bone labeling

Standard

Characterization of eight different tetracyclines: advances in fluorescence bone labeling. / Pautke, Christoph; Vogt, Stephan; Kreutzer, Kilian; Haczek, Cornelia; Wexel, Gabriele; Kolk, Andreas; Imhoff, Andreas B; Zitzelsberger, Horst; Milz, Stefan; Tischer, Thomas.

in: J ANAT, Jahrgang 217, Nr. 1, 07.2010, S. 76-82.

Publikationen: SCORING: Beitrag in Fachzeitschrift/ZeitungSCORING: ZeitschriftenaufsatzForschungBegutachtung

Harvard

Pautke, C, Vogt, S, Kreutzer, K, Haczek, C, Wexel, G, Kolk, A, Imhoff, AB, Zitzelsberger, H, Milz, S & Tischer, T 2010, 'Characterization of eight different tetracyclines: advances in fluorescence bone labeling', J ANAT, Jg. 217, Nr. 1, S. 76-82. https://doi.org/10.1111/j.1469-7580.2010.01237.x

APA

Pautke, C., Vogt, S., Kreutzer, K., Haczek, C., Wexel, G., Kolk, A., Imhoff, A. B., Zitzelsberger, H., Milz, S., & Tischer, T. (2010). Characterization of eight different tetracyclines: advances in fluorescence bone labeling. J ANAT, 217(1), 76-82. https://doi.org/10.1111/j.1469-7580.2010.01237.x

Vancouver

Bibtex

@article{707fd720fd41437086c085dcf8a1752f,
title = "Characterization of eight different tetracyclines: advances in fluorescence bone labeling",
abstract = "Polychrome sequential labeling with fluorochromes is a standard technique for the investigation of bone formation and regeneration processes in vivo. However, for human application, only tetracycline and its derivates are approved as fluorochromes. Therefore, the aim of this study was to determine the fluorescence characteristics of the different tetracycline derivates to assess the feasibility of sequential in vivo bone labeling using distinguishable fluorochromes. Eight different tetracycline derivates were injected subcutaneously into growing rats as a single dose or sequentially in different combinations. After preparation of resin-embedded undecalcified bone sections, the fluorescence properties of the tetracycline derivates in bone were analyzed using conventional fluorescence microscopy, spectral image analysis and confocal laser scanning microscopy. Each tetracycline derivate exhibited a characteristic fluorescence spectrum, but the differences between them were small. Chlortetracycline could be discriminated reliably from all other derivates and could therefore be combined with any other tetracycline derivate for reliably distinguishable double labeling. Tetracycline itself exhibited the brightest fluorescence of all the investigated derivates. Interestingly, in conventional microscopy the same tetracycline derivative can appear in different colours to the human eye, even if spectral analysis confirmed identical emission peaks. In conclusion, the data suggest that fluorescence double labeling of bone is feasible using appropriate tetracycline derivates in combination with spectral imaging modalities.",
keywords = "Animals, Bone and Bones, Feasibility Studies, Fluorescent Dyes, Image Processing, Computer-Assisted, Male, Microscopy, Confocal, Microscopy, Fluorescence, Rats, Rats, Wistar, Tetracyclines",
author = "Christoph Pautke and Stephan Vogt and Kilian Kreutzer and Cornelia Haczek and Gabriele Wexel and Andreas Kolk and Imhoff, {Andreas B} and Horst Zitzelsberger and Stefan Milz and Thomas Tischer",
year = "2010",
month = jul,
doi = "10.1111/j.1469-7580.2010.01237.x",
language = "English",
volume = "217",
pages = "76--82",
journal = "J ANAT",
issn = "0021-8782",
publisher = "Wiley-Blackwell",
number = "1",

}

RIS

TY - JOUR

T1 - Characterization of eight different tetracyclines: advances in fluorescence bone labeling

AU - Pautke, Christoph

AU - Vogt, Stephan

AU - Kreutzer, Kilian

AU - Haczek, Cornelia

AU - Wexel, Gabriele

AU - Kolk, Andreas

AU - Imhoff, Andreas B

AU - Zitzelsberger, Horst

AU - Milz, Stefan

AU - Tischer, Thomas

PY - 2010/7

Y1 - 2010/7

N2 - Polychrome sequential labeling with fluorochromes is a standard technique for the investigation of bone formation and regeneration processes in vivo. However, for human application, only tetracycline and its derivates are approved as fluorochromes. Therefore, the aim of this study was to determine the fluorescence characteristics of the different tetracycline derivates to assess the feasibility of sequential in vivo bone labeling using distinguishable fluorochromes. Eight different tetracycline derivates were injected subcutaneously into growing rats as a single dose or sequentially in different combinations. After preparation of resin-embedded undecalcified bone sections, the fluorescence properties of the tetracycline derivates in bone were analyzed using conventional fluorescence microscopy, spectral image analysis and confocal laser scanning microscopy. Each tetracycline derivate exhibited a characteristic fluorescence spectrum, but the differences between them were small. Chlortetracycline could be discriminated reliably from all other derivates and could therefore be combined with any other tetracycline derivate for reliably distinguishable double labeling. Tetracycline itself exhibited the brightest fluorescence of all the investigated derivates. Interestingly, in conventional microscopy the same tetracycline derivative can appear in different colours to the human eye, even if spectral analysis confirmed identical emission peaks. In conclusion, the data suggest that fluorescence double labeling of bone is feasible using appropriate tetracycline derivates in combination with spectral imaging modalities.

AB - Polychrome sequential labeling with fluorochromes is a standard technique for the investigation of bone formation and regeneration processes in vivo. However, for human application, only tetracycline and its derivates are approved as fluorochromes. Therefore, the aim of this study was to determine the fluorescence characteristics of the different tetracycline derivates to assess the feasibility of sequential in vivo bone labeling using distinguishable fluorochromes. Eight different tetracycline derivates were injected subcutaneously into growing rats as a single dose or sequentially in different combinations. After preparation of resin-embedded undecalcified bone sections, the fluorescence properties of the tetracycline derivates in bone were analyzed using conventional fluorescence microscopy, spectral image analysis and confocal laser scanning microscopy. Each tetracycline derivate exhibited a characteristic fluorescence spectrum, but the differences between them were small. Chlortetracycline could be discriminated reliably from all other derivates and could therefore be combined with any other tetracycline derivate for reliably distinguishable double labeling. Tetracycline itself exhibited the brightest fluorescence of all the investigated derivates. Interestingly, in conventional microscopy the same tetracycline derivative can appear in different colours to the human eye, even if spectral analysis confirmed identical emission peaks. In conclusion, the data suggest that fluorescence double labeling of bone is feasible using appropriate tetracycline derivates in combination with spectral imaging modalities.

KW - Animals

KW - Bone and Bones

KW - Feasibility Studies

KW - Fluorescent Dyes

KW - Image Processing, Computer-Assisted

KW - Male

KW - Microscopy, Confocal

KW - Microscopy, Fluorescence

KW - Rats

KW - Rats, Wistar

KW - Tetracyclines

U2 - 10.1111/j.1469-7580.2010.01237.x

DO - 10.1111/j.1469-7580.2010.01237.x

M3 - SCORING: Journal article

C2 - 20456523

VL - 217

SP - 76

EP - 82

JO - J ANAT

JF - J ANAT

SN - 0021-8782

IS - 1

ER -