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Characterization of dimethylguanosine, phenylethylamine, and phenylacetic acid as inhibitors of Ca2+ ATPase in end-stage renal failure

Standard

Characterization of dimethylguanosine, phenylethylamine, and phenylacetic acid as inhibitors of Ca2+ ATPase in end-stage renal failure. / Jankowski, J; Luftmann, H; Tepel, M; Leibfritz, D; Zidek, W; Schlüter, H.

in: J AM SOC NEPHROL, Jahrgang 9, Nr. 7, 07.1998, S. 1249-57.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Jankowski, J, Luftmann, H, Tepel, M, Leibfritz, D, Zidek, W & Schlüter, H 1998, 'Characterization of dimethylguanosine, phenylethylamine, and phenylacetic acid as inhibitors of Ca2+ ATPase in end-stage renal failure', J AM SOC NEPHROL, Jg. 9, Nr. 7, S. 1249-57.

APA

Jankowski, J., Luftmann, H., Tepel, M., Leibfritz, D., Zidek, W., & Schlüter, H. (1998). Characterization of dimethylguanosine, phenylethylamine, and phenylacetic acid as inhibitors of Ca2+ ATPase in end-stage renal failure. J AM SOC NEPHROL, 9(7), 1249-57.

Vancouver

Jankowski J, Luftmann H, Tepel M, Leibfritz D, Zidek W, Schlüter H. Characterization of dimethylguanosine, phenylethylamine, and phenylacetic acid as inhibitors of Ca2+ ATPase in end-stage renal failure. J AM SOC NEPHROL. 1998 Jul;9(7):1249-57.

Bibtex

@article{4f25b8457c4e46e682b51cb0c6ded5d9,
title = "Characterization of dimethylguanosine, phenylethylamine, and phenylacetic acid as inhibitors of Ca2+ ATPase in end-stage renal failure",
abstract = "The activity of the plasma membrane Ca2+ ATPase of chronic renal failure patients is decreased by circulating inhibitors yet to be characterized. In this study, inhibitors of Ca2+ ATPase were isolated from ultrafiltrate of patients with end-stage renal failure. They were identified as dimethylguanosine, phenylethylamine, and phenylacetic acid by chromatography and mass spectrometry. Ca2+ ATPase activity was measured spectrophotometrically as the difference in hydrolysis of ATP in the presence and absence of Ca2+ with different concentrations of ATP and the isolated substances. All of the identified compounds are sufficiently lipophilic to penetrate the blood-brain barrier and to accumulate in cerebral tissue. The inhibitory effects of these agents were additive. The apparent K(m) values for ATP and Ca2+ were not altered by these substances, suggesting a noncompetitive mechanism of inhibition. In plasma of healthy subjects, the substances were not detectable. The Ca2+ ATPase inhibitors identified may play a role in the pathophysiology of end-stage renal failure and, potentially, in monitoring toxic effects on cellular Ca2+ metabolism in renal failure.",
keywords = "Adult, Calcium-Transporting ATPases, Cell Membrane, Erythrocytes, Female, Guanosine, Humans, Kidney Failure, Chronic, Magnetic Resonance Spectroscopy, Male, Mass Spectrometry, Middle Aged, Phenethylamines, Phenylacetates, Reference Values, Statistics, Nonparametric, Clinical Trial, Journal Article",
author = "J Jankowski and H Luftmann and M Tepel and D Leibfritz and W Zidek and H Schl{\"u}ter",
year = "1998",
month = jul,
language = "English",
volume = "9",
pages = "1249--57",
journal = "J AM SOC NEPHROL",
issn = "1046-6673",
publisher = "American Society of Nephrology",
number = "7",

}

RIS

TY - JOUR

T1 - Characterization of dimethylguanosine, phenylethylamine, and phenylacetic acid as inhibitors of Ca2+ ATPase in end-stage renal failure

AU - Jankowski, J

AU - Luftmann, H

AU - Tepel, M

AU - Leibfritz, D

AU - Zidek, W

AU - Schlüter, H

PY - 1998/7

Y1 - 1998/7

N2 - The activity of the plasma membrane Ca2+ ATPase of chronic renal failure patients is decreased by circulating inhibitors yet to be characterized. In this study, inhibitors of Ca2+ ATPase were isolated from ultrafiltrate of patients with end-stage renal failure. They were identified as dimethylguanosine, phenylethylamine, and phenylacetic acid by chromatography and mass spectrometry. Ca2+ ATPase activity was measured spectrophotometrically as the difference in hydrolysis of ATP in the presence and absence of Ca2+ with different concentrations of ATP and the isolated substances. All of the identified compounds are sufficiently lipophilic to penetrate the blood-brain barrier and to accumulate in cerebral tissue. The inhibitory effects of these agents were additive. The apparent K(m) values for ATP and Ca2+ were not altered by these substances, suggesting a noncompetitive mechanism of inhibition. In plasma of healthy subjects, the substances were not detectable. The Ca2+ ATPase inhibitors identified may play a role in the pathophysiology of end-stage renal failure and, potentially, in monitoring toxic effects on cellular Ca2+ metabolism in renal failure.

AB - The activity of the plasma membrane Ca2+ ATPase of chronic renal failure patients is decreased by circulating inhibitors yet to be characterized. In this study, inhibitors of Ca2+ ATPase were isolated from ultrafiltrate of patients with end-stage renal failure. They were identified as dimethylguanosine, phenylethylamine, and phenylacetic acid by chromatography and mass spectrometry. Ca2+ ATPase activity was measured spectrophotometrically as the difference in hydrolysis of ATP in the presence and absence of Ca2+ with different concentrations of ATP and the isolated substances. All of the identified compounds are sufficiently lipophilic to penetrate the blood-brain barrier and to accumulate in cerebral tissue. The inhibitory effects of these agents were additive. The apparent K(m) values for ATP and Ca2+ were not altered by these substances, suggesting a noncompetitive mechanism of inhibition. In plasma of healthy subjects, the substances were not detectable. The Ca2+ ATPase inhibitors identified may play a role in the pathophysiology of end-stage renal failure and, potentially, in monitoring toxic effects on cellular Ca2+ metabolism in renal failure.

KW - Adult

KW - Calcium-Transporting ATPases

KW - Cell Membrane

KW - Erythrocytes

KW - Female

KW - Guanosine

KW - Humans

KW - Kidney Failure, Chronic

KW - Magnetic Resonance Spectroscopy

KW - Male

KW - Mass Spectrometry

KW - Middle Aged

KW - Phenethylamines

KW - Phenylacetates

KW - Reference Values

KW - Statistics, Nonparametric

KW - Clinical Trial

KW - Journal Article

M3 - SCORING: Journal article

C2 - 9644635

VL - 9

SP - 1249

EP - 1257

JO - J AM SOC NEPHROL

JF - J AM SOC NEPHROL

SN - 1046-6673

IS - 7

ER -