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Characterization of a Standardized Ex-vivo Porcine Model to Assess Short Term Intraocular Pressure Changes and Trabecular Meshwork Vitality After Pars Plana Vitrectomy with Different Silicone Oil and BSS Tamponades

Standard

Characterization of a Standardized Ex-vivo Porcine Model to Assess Short Term Intraocular Pressure Changes and Trabecular Meshwork Vitality After Pars Plana Vitrectomy with Different Silicone Oil and BSS Tamponades. / Ebner, Martina; Mariacher, Siegfried; Hurst, José; Szurman, Peter; Schnichels, Sven; Spitzer, Martin S; Januschowski, Kai.

in: CURR EYE RES, Jahrgang 42, Nr. 8, 08.2017, S. 1130-1135.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Ebner, M, Mariacher, S, Hurst, J, Szurman, P, Schnichels, S, Spitzer, MS & Januschowski, K 2017, 'Characterization of a Standardized Ex-vivo Porcine Model to Assess Short Term Intraocular Pressure Changes and Trabecular Meshwork Vitality After Pars Plana Vitrectomy with Different Silicone Oil and BSS Tamponades', CURR EYE RES, Jg. 42, Nr. 8, S. 1130-1135. https://doi.org/10.1080/02713683.2017.1297461

APA

Ebner, M., Mariacher, S., Hurst, J., Szurman, P., Schnichels, S., Spitzer, M. S., & Januschowski, K. (2017). Characterization of a Standardized Ex-vivo Porcine Model to Assess Short Term Intraocular Pressure Changes and Trabecular Meshwork Vitality After Pars Plana Vitrectomy with Different Silicone Oil and BSS Tamponades. CURR EYE RES, 42(8), 1130-1135. https://doi.org/10.1080/02713683.2017.1297461

Vancouver

Ebner M, Mariacher S, Hurst J, Szurman P, Schnichels S, Spitzer MS et al. Characterization of a Standardized Ex-vivo Porcine Model to Assess Short Term Intraocular Pressure Changes and Trabecular Meshwork Vitality After Pars Plana Vitrectomy with Different Silicone Oil and BSS Tamponades. CURR EYE RES. 2017 Aug;42(8):1130-1135. https://doi.org/10.1080/02713683.2017.1297461

Bibtex

@article{943de805ee454be68d8a319ed69bbb0b,
title = "Characterization of a Standardized Ex-vivo Porcine Model to Assess Short Term Intraocular Pressure Changes and Trabecular Meshwork Vitality After Pars Plana Vitrectomy with Different Silicone Oil and BSS Tamponades",
abstract = "PURPOSE: The aim of this study was to characterize a standardized porcine ex-vivo testing system for intraocular pressure (IOP) monitoring after vitrectomy with different endotamponades.METHODS: Twenty-four pig eyes, six per endotamponade group were obtained immediately postmortem. After pars plana vitrectomy, vitreous substitutes (silicone oil 1000 mPas, 2000 mPas, 5000 mPas, and Balanced Salt Solution (BSS)) were instillated and IOP was observed over 24-hours. Infusion pumps with Dulbecco's Modified Eagle Medium (DMEM) simulated a constant aqueous humor circulation. A histological examination of the trabecular meshwork with DAPI- and TUNEL-staining was performed to detect the amount of apoptotic cells.RESULTS: TUNEL-assay showed a mean cell death rate of 3.78% (SD ± 1.46%) for silicone oil endotamponades compared to 5.05% (SD ± 2.18%) in BSS group. One-way ANOVA (p = 0.425) showed no significant difference between both groups. Mean IOP in silicone oil endotamponades was 9.50 mmHg (SD ± 1.68 mmHg) at baseline, 13.23 mmHg (SD ± 0.79 mmHg) after 1 hour, 18.46 mmHg (SD ± 2.13 mmHg) after 12 hours and 15.51 mmHg (SD ± 2.82 mmHg) 24 hours after instillation. A comparison of all silicone oil groups (one-way ANOVA, Bonferroni post-hoc test, p = 0.269 to 1.000) didn't reveal significant differences in mean IOP.CONCLUSION: The standardized ex-vivo porcine model represents an effective alternative to the in-vivo testing in animals. Maintaining the trabecular and uveoscleral outflow pathway enables a pseudo in-vivo analysis.",
keywords = "Acetates, Animals, Disease Models, Animal, Drug Combinations, Endotamponade, Equipment Design, Follow-Up Studies, In Situ Nick-End Labeling, Intraocular Pressure, Minerals, Ocular Hypertension, Postoperative Complications, Postoperative Period, Retinal Detachment, Silicone Oils, Sodium Chloride, Swine, Time Factors, Tonometry, Ocular, Trabecular Meshwork, Vitrectomy, Journal Article",
author = "Martina Ebner and Siegfried Mariacher and Jos{\'e} Hurst and Peter Szurman and Sven Schnichels and Spitzer, {Martin S} and Kai Januschowski",
year = "2017",
month = aug,
doi = "10.1080/02713683.2017.1297461",
language = "English",
volume = "42",
pages = "1130--1135",
journal = "CURR EYE RES",
issn = "0271-3683",
publisher = "Taylor & Francis",
number = "8",

}

RIS

TY - JOUR

T1 - Characterization of a Standardized Ex-vivo Porcine Model to Assess Short Term Intraocular Pressure Changes and Trabecular Meshwork Vitality After Pars Plana Vitrectomy with Different Silicone Oil and BSS Tamponades

AU - Ebner, Martina

AU - Mariacher, Siegfried

AU - Hurst, José

AU - Szurman, Peter

AU - Schnichels, Sven

AU - Spitzer, Martin S

AU - Januschowski, Kai

PY - 2017/8

Y1 - 2017/8

N2 - PURPOSE: The aim of this study was to characterize a standardized porcine ex-vivo testing system for intraocular pressure (IOP) monitoring after vitrectomy with different endotamponades.METHODS: Twenty-four pig eyes, six per endotamponade group were obtained immediately postmortem. After pars plana vitrectomy, vitreous substitutes (silicone oil 1000 mPas, 2000 mPas, 5000 mPas, and Balanced Salt Solution (BSS)) were instillated and IOP was observed over 24-hours. Infusion pumps with Dulbecco's Modified Eagle Medium (DMEM) simulated a constant aqueous humor circulation. A histological examination of the trabecular meshwork with DAPI- and TUNEL-staining was performed to detect the amount of apoptotic cells.RESULTS: TUNEL-assay showed a mean cell death rate of 3.78% (SD ± 1.46%) for silicone oil endotamponades compared to 5.05% (SD ± 2.18%) in BSS group. One-way ANOVA (p = 0.425) showed no significant difference between both groups. Mean IOP in silicone oil endotamponades was 9.50 mmHg (SD ± 1.68 mmHg) at baseline, 13.23 mmHg (SD ± 0.79 mmHg) after 1 hour, 18.46 mmHg (SD ± 2.13 mmHg) after 12 hours and 15.51 mmHg (SD ± 2.82 mmHg) 24 hours after instillation. A comparison of all silicone oil groups (one-way ANOVA, Bonferroni post-hoc test, p = 0.269 to 1.000) didn't reveal significant differences in mean IOP.CONCLUSION: The standardized ex-vivo porcine model represents an effective alternative to the in-vivo testing in animals. Maintaining the trabecular and uveoscleral outflow pathway enables a pseudo in-vivo analysis.

AB - PURPOSE: The aim of this study was to characterize a standardized porcine ex-vivo testing system for intraocular pressure (IOP) monitoring after vitrectomy with different endotamponades.METHODS: Twenty-four pig eyes, six per endotamponade group were obtained immediately postmortem. After pars plana vitrectomy, vitreous substitutes (silicone oil 1000 mPas, 2000 mPas, 5000 mPas, and Balanced Salt Solution (BSS)) were instillated and IOP was observed over 24-hours. Infusion pumps with Dulbecco's Modified Eagle Medium (DMEM) simulated a constant aqueous humor circulation. A histological examination of the trabecular meshwork with DAPI- and TUNEL-staining was performed to detect the amount of apoptotic cells.RESULTS: TUNEL-assay showed a mean cell death rate of 3.78% (SD ± 1.46%) for silicone oil endotamponades compared to 5.05% (SD ± 2.18%) in BSS group. One-way ANOVA (p = 0.425) showed no significant difference between both groups. Mean IOP in silicone oil endotamponades was 9.50 mmHg (SD ± 1.68 mmHg) at baseline, 13.23 mmHg (SD ± 0.79 mmHg) after 1 hour, 18.46 mmHg (SD ± 2.13 mmHg) after 12 hours and 15.51 mmHg (SD ± 2.82 mmHg) 24 hours after instillation. A comparison of all silicone oil groups (one-way ANOVA, Bonferroni post-hoc test, p = 0.269 to 1.000) didn't reveal significant differences in mean IOP.CONCLUSION: The standardized ex-vivo porcine model represents an effective alternative to the in-vivo testing in animals. Maintaining the trabecular and uveoscleral outflow pathway enables a pseudo in-vivo analysis.

KW - Acetates

KW - Animals

KW - Disease Models, Animal

KW - Drug Combinations

KW - Endotamponade

KW - Equipment Design

KW - Follow-Up Studies

KW - In Situ Nick-End Labeling

KW - Intraocular Pressure

KW - Minerals

KW - Ocular Hypertension

KW - Postoperative Complications

KW - Postoperative Period

KW - Retinal Detachment

KW - Silicone Oils

KW - Sodium Chloride

KW - Swine

KW - Time Factors

KW - Tonometry, Ocular

KW - Trabecular Meshwork

KW - Vitrectomy

KW - Journal Article

U2 - 10.1080/02713683.2017.1297461

DO - 10.1080/02713683.2017.1297461

M3 - SCORING: Journal article

C2 - 28441060

VL - 42

SP - 1130

EP - 1135

JO - CURR EYE RES

JF - CURR EYE RES

SN - 0271-3683

IS - 8

ER -