PortalPublikationenChapter 4. Screening phage-display Peptide libraries for vas...

Chapter 4. Screening phage-display Peptide libraries for vascular targeted peptides

Standard

Chapter 4. Screening phage-display Peptide libraries for vascular targeted peptides. / Trepel, Martin; Pasqualini, Renata; Arap, Wadih.

in: METHOD ENZYMOL, Jahrgang 445, 2008, S. 83-106.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Trepel, M, Pasqualini, R & Arap, W 2008, 'Chapter 4. Screening phage-display Peptide libraries for vascular targeted peptides', METHOD ENZYMOL, Jg. 445, S. 83-106. https://doi.org/10.1016/S0076-6879(08)03004-8

APA

Trepel, M., Pasqualini, R., & Arap, W. (2008). Chapter 4. Screening phage-display Peptide libraries for vascular targeted peptides. METHOD ENZYMOL, 445, 83-106. https://doi.org/10.1016/S0076-6879(08)03004-8

Vancouver

Trepel M, Pasqualini R, Arap W. Chapter 4. Screening phage-display Peptide libraries for vascular targeted peptides. METHOD ENZYMOL. 2008;445:83-106. https://doi.org/10.1016/S0076-6879(08)03004-8

Bibtex

@article{edb33120e8a7403a99c3e9533678e358,
title = "Chapter 4. Screening phage-display Peptide libraries for vascular targeted peptides",
abstract = "Depending on their physiologic location and functional state, vascular endothelial cells express surface receptors differentially. Recognition of this molecular diversity is essential for the development of targeted therapies. Random phage display peptide libraries can be selected in vitro on recombinant proteins or on intact cells. After systemic injection, selection can be performed in animals and humans in vivo for the isolation of ligands for tissue-specific receptors. For the screening of libraries on intact cells or tissues, no a priori knowledge of the targeted receptor is needed, as the recovered peptide ligands can identify their corresponding receptors. Furthermore, the isolated peptides can be used to target therapeutic chemicals, biologicals, gene therapy vectors, or diagnostic compounds to specific tissues in vivo. Protocols for the screening of phage libraries in these three settings--on proteins, on cells in vitro and in the living animal--are described in this chapter.",
keywords = "Animals, Endothelial Cells, Humans, Peptide Library, Peptides, Protein Binding, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.",
author = "Martin Trepel and Renata Pasqualini and Wadih Arap",
year = "2008",
doi = "10.1016/S0076-6879(08)03004-8",
language = "English",
volume = "445",
pages = "83--106",

}

RIS

TY - JOUR

T1 - Chapter 4. Screening phage-display Peptide libraries for vascular targeted peptides

AU - Trepel, Martin

AU - Pasqualini, Renata

AU - Arap, Wadih

PY - 2008

Y1 - 2008

N2 - Depending on their physiologic location and functional state, vascular endothelial cells express surface receptors differentially. Recognition of this molecular diversity is essential for the development of targeted therapies. Random phage display peptide libraries can be selected in vitro on recombinant proteins or on intact cells. After systemic injection, selection can be performed in animals and humans in vivo for the isolation of ligands for tissue-specific receptors. For the screening of libraries on intact cells or tissues, no a priori knowledge of the targeted receptor is needed, as the recovered peptide ligands can identify their corresponding receptors. Furthermore, the isolated peptides can be used to target therapeutic chemicals, biologicals, gene therapy vectors, or diagnostic compounds to specific tissues in vivo. Protocols for the screening of phage libraries in these three settings--on proteins, on cells in vitro and in the living animal--are described in this chapter.

AB - Depending on their physiologic location and functional state, vascular endothelial cells express surface receptors differentially. Recognition of this molecular diversity is essential for the development of targeted therapies. Random phage display peptide libraries can be selected in vitro on recombinant proteins or on intact cells. After systemic injection, selection can be performed in animals and humans in vivo for the isolation of ligands for tissue-specific receptors. For the screening of libraries on intact cells or tissues, no a priori knowledge of the targeted receptor is needed, as the recovered peptide ligands can identify their corresponding receptors. Furthermore, the isolated peptides can be used to target therapeutic chemicals, biologicals, gene therapy vectors, or diagnostic compounds to specific tissues in vivo. Protocols for the screening of phage libraries in these three settings--on proteins, on cells in vitro and in the living animal--are described in this chapter.

KW - Animals

KW - Endothelial Cells

KW - Humans

KW - Peptide Library

KW - Peptides

KW - Protein Binding

KW - Journal Article

KW - Research Support, N.I.H., Extramural

KW - Research Support, Non-U.S. Gov't

KW - Research Support, U.S. Gov't, Non-P.H.S.

U2 - 10.1016/S0076-6879(08)03004-8

DO - 10.1016/S0076-6879(08)03004-8

M3 - SCORING: Journal article

C2 - 19022056

VL - 445

SP - 83

EP - 106

ER -