Centrosome-dependent microtubule modifications set the conditions for axon formation

Durga Praveen Meka; Oliver Kobler; Shuai Hong; Carina Meta Friedrich; Souhaila Wuesthoff; Melad Henis; Birgit Schwanke; Christoph Krisp; Nessa Schmuelling; René Rueter; Tabitha Ruecker; Ewelina Betleja; Tao Cheng; Moe R Mahjoub; Peter Soba; Hartmut Schlüter; Eugenio F Fornasiero; Froylan Calderon de Anda

doi:10.1016/j.celrep.2022.110686

Centrosome-dependent microtubule modifications set the conditions for axon formation

Standard

Centrosome-dependent microtubule modifications set the conditions for axon formation. / Meka, Durga Praveen; Kobler, Oliver; Hong, Shuai; Friedrich, Carina Meta; Wuesthoff, Souhaila; Henis, Melad; Schwanke, Birgit; Krisp, Christoph; Schmuelling, Nessa; Rueter, René; Ruecker, Tabitha; Betleja, Ewelina; Cheng, Tao; Mahjoub, Moe R; Soba, Peter; Schlüter, Hartmut; Fornasiero, Eugenio F; Calderon de Anda, Froylan.

in: CELL REP, Jahrgang 39, Nr. 3, 19.04.2022, S. 110686.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Meka, DP, Kobler, O, Hong, S, Friedrich, CM, Wuesthoff, S, Henis, M, Schwanke, B, Krisp, C, Schmuelling, N, Rueter, R, Ruecker, T, Betleja, E, Cheng, T, Mahjoub, MR, Soba, P, Schlüter, H, Fornasiero, EF & Calderon de Anda, F 2022, 'Centrosome-dependent microtubule modifications set the conditions for axon formation', CELL REP, Jg. 39, Nr. 3, S. 110686. https://doi.org/10.1016/j.celrep.2022.110686

APA

Meka, D. P., Kobler, O., Hong, S., Friedrich, C. M., Wuesthoff, S., Henis, M., Schwanke, B., Krisp, C., Schmuelling, N., Rueter, R., Ruecker, T., Betleja, E., Cheng, T., Mahjoub, M. R., Soba, P., Schlüter, H., Fornasiero, E. F., & Calderon de Anda, F. (2022). Centrosome-dependent microtubule modifications set the conditions for axon formation. CELL REP, 39(3), 110686. https://doi.org/10.1016/j.celrep.2022.110686

Vancouver

Meka DP, Kobler O, Hong S, Friedrich CM, Wuesthoff S, Henis M et al. Centrosome-dependent microtubule modifications set the conditions for axon formation. CELL REP. 2022 Apr 19;39(3):110686. https://doi.org/10.1016/j.celrep.2022.110686

Bibtex

@article{890264e275854dcc82fc25ab0d129bb2,

title = "Centrosome-dependent microtubule modifications set the conditions for axon formation",

abstract = "Microtubule (MT) modifications are critical during axon development, with stable MTs populating the axon. How these modifications are spatially coordinated is unclear. Here, via high-resolution microscopy, we show that early developing neurons have fewer somatic acetylated MTs restricted near the centrosome. At later stages, however, acetylated MTs spread out in soma and concentrate in growing axon. Live imaging in early plated neurons of the MT plus-end protein, EB3, show increased displacement and growth rate near the MTOC, suggesting local differences that might support axon selection. Moreover, F-actin disruption in early developing neurons, which show fewer somatic acetylated MTs, does not induce multiple axons, unlike later stages. Overexpression of centrosomal protein 120 (Cep120), which promotes MT acetylation/stabilization, induces multiple axons, while its knockdown downregulates proteins modulating MT dynamics and stability, hampering axon formation. Collectively, we show how centrosome-dependent MT modifications contribute to axon formation.",

keywords = "Actin Cytoskeleton, Axons/metabolism, Centrosome/metabolism, Microtubule-Associated Proteins/metabolism, Microtubules/metabolism, Neurons/metabolism",

author = "Meka, {Durga Praveen} and Oliver Kobler and Shuai Hong and Friedrich, {Carina Meta} and Souhaila Wuesthoff and Melad Henis and Birgit Schwanke and Christoph Krisp and Nessa Schmuelling and Ren{\'e} Rueter and Tabitha Ruecker and Ewelina Betleja and Tao Cheng and Mahjoub, {Moe R} and Peter Soba and Hartmut Schl{\"u}ter and Fornasiero, {Eugenio F} and {Calderon de Anda}, Froylan",

year = "2022",

month = apr,

day = "19",

doi = "10.1016/j.celrep.2022.110686",

language = "English",

volume = "39",

pages = "110686",

journal = "CELL REP",

issn = "2211-1247",

publisher = "Elsevier",

number = "3",

}

RIS

TY - JOUR

T1 - Centrosome-dependent microtubule modifications set the conditions for axon formation

AU - Meka, Durga Praveen

AU - Kobler, Oliver

AU - Hong, Shuai

AU - Friedrich, Carina Meta

AU - Wuesthoff, Souhaila

AU - Henis, Melad

AU - Schwanke, Birgit

AU - Krisp, Christoph

AU - Schmuelling, Nessa

AU - Rueter, René

AU - Ruecker, Tabitha

AU - Betleja, Ewelina

AU - Cheng, Tao

AU - Mahjoub, Moe R

AU - Soba, Peter

AU - Schlüter, Hartmut

AU - Fornasiero, Eugenio F

AU - Calderon de Anda, Froylan

PY - 2022/4/19

Y1 - 2022/4/19

N2 - Microtubule (MT) modifications are critical during axon development, with stable MTs populating the axon. How these modifications are spatially coordinated is unclear. Here, via high-resolution microscopy, we show that early developing neurons have fewer somatic acetylated MTs restricted near the centrosome. At later stages, however, acetylated MTs spread out in soma and concentrate in growing axon. Live imaging in early plated neurons of the MT plus-end protein, EB3, show increased displacement and growth rate near the MTOC, suggesting local differences that might support axon selection. Moreover, F-actin disruption in early developing neurons, which show fewer somatic acetylated MTs, does not induce multiple axons, unlike later stages. Overexpression of centrosomal protein 120 (Cep120), which promotes MT acetylation/stabilization, induces multiple axons, while its knockdown downregulates proteins modulating MT dynamics and stability, hampering axon formation. Collectively, we show how centrosome-dependent MT modifications contribute to axon formation.

AB - Microtubule (MT) modifications are critical during axon development, with stable MTs populating the axon. How these modifications are spatially coordinated is unclear. Here, via high-resolution microscopy, we show that early developing neurons have fewer somatic acetylated MTs restricted near the centrosome. At later stages, however, acetylated MTs spread out in soma and concentrate in growing axon. Live imaging in early plated neurons of the MT plus-end protein, EB3, show increased displacement and growth rate near the MTOC, suggesting local differences that might support axon selection. Moreover, F-actin disruption in early developing neurons, which show fewer somatic acetylated MTs, does not induce multiple axons, unlike later stages. Overexpression of centrosomal protein 120 (Cep120), which promotes MT acetylation/stabilization, induces multiple axons, while its knockdown downregulates proteins modulating MT dynamics and stability, hampering axon formation. Collectively, we show how centrosome-dependent MT modifications contribute to axon formation.

KW - Actin Cytoskeleton

KW - Axons/metabolism

KW - Centrosome/metabolism

KW - Microtubule-Associated Proteins/metabolism

KW - Microtubules/metabolism

KW - Neurons/metabolism

U2 - 10.1016/j.celrep.2022.110686

DO - 10.1016/j.celrep.2022.110686

M3 - SCORING: Journal article

C2 - 35443171

VL - 39

SP - 110686

JO - CELL REP

JF - CELL REP

SN - 2211-1247

IS - 3

ER -