CD38 downregulation modulates NAD+ and NADP(H) levels in thermogenic adipose tissues
Standard
CD38 downregulation modulates NAD+ and NADP(H) levels in thermogenic adipose tissues. / Benzi, Andrea; Sturla, Laura; Heine, Markus; Fischer, Alexander W; Spinelli, Sonia; Magnone, Mirko; Sociali, Giovanna; Parodi, Alessia; Fenoglio, Daniela; Emionite, Laura; Koch-Nolte, Friedrich; Mittrücker, Hans-Willi; Guse, Andreas H; De Flora, Antonio; Zocchi, Elena; Heeren, Joerg; Bruzzone, Santina.
in: BBA-MOL CELL BIOL L, Jahrgang 1866, Nr. 1, 01.2021, S. 158819.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - CD38 downregulation modulates NAD+ and NADP(H) levels in thermogenic adipose tissues
AU - Benzi, Andrea
AU - Sturla, Laura
AU - Heine, Markus
AU - Fischer, Alexander W
AU - Spinelli, Sonia
AU - Magnone, Mirko
AU - Sociali, Giovanna
AU - Parodi, Alessia
AU - Fenoglio, Daniela
AU - Emionite, Laura
AU - Koch-Nolte, Friedrich
AU - Mittrücker, Hans-Willi
AU - Guse, Andreas H
AU - De Flora, Antonio
AU - Zocchi, Elena
AU - Heeren, Joerg
AU - Bruzzone, Santina
N1 - Copyright © 2020 Elsevier B.V. All rights reserved.
PY - 2021/1
Y1 - 2021/1
N2 - Different strategies to boost NAD+ levels are considered promising means to promote healthy aging and ameliorate dysfunctional metabolism. CD38 is a NAD+-dependent enzyme involved in the regulation of different cell functions. In the context of systemic energy metabolism, it has been demonstrated that brown adipocytes, the parenchymal cells of brown adipose tissue (BAT) as well as beige adipocytes that emerge in white adipose tissue (WAT) depots in response to catabolic conditions, are important to maintain metabolic homeostasis. In this study we aim to understand the functional relevance of CD38 for NAD+ and energy metabolism in BAT and WAT, also using a CD38-/- mouse model. During cold exposure, an increase in NAD+ levels occurred in BAT of wild type mice, together with a marked downregulation of CD38, as detected at the mRNA and protein level. CD38 downregulation was observed also in WAT of cold-exposed mice, where it was accompanied by a strong increase in NADP(H) levels. Accordingly, NAD kinase and glucose-6-phosphate dehydrogenase activities were enhanced in WAT (but not in BAT). Increased NAD+ levels were observed in BAT/WAT from CD38-/- compared with wild type mice, in line with CD38 being a major NAD+-consumer in AT. CD38-/- mice kept at 6 °C had higher levels of Ucp1 and Pgc-1α in BAT and WAT, and increased levels of phosphorylated hormone-sensitive lipase in BAT, compared with wild type mice. These results demonstrate that CD38, by modulating cellular NAD(P)+ levels, is involved in the regulation of thermogenic responses in cold-activated BAT and WAT.
AB - Different strategies to boost NAD+ levels are considered promising means to promote healthy aging and ameliorate dysfunctional metabolism. CD38 is a NAD+-dependent enzyme involved in the regulation of different cell functions. In the context of systemic energy metabolism, it has been demonstrated that brown adipocytes, the parenchymal cells of brown adipose tissue (BAT) as well as beige adipocytes that emerge in white adipose tissue (WAT) depots in response to catabolic conditions, are important to maintain metabolic homeostasis. In this study we aim to understand the functional relevance of CD38 for NAD+ and energy metabolism in BAT and WAT, also using a CD38-/- mouse model. During cold exposure, an increase in NAD+ levels occurred in BAT of wild type mice, together with a marked downregulation of CD38, as detected at the mRNA and protein level. CD38 downregulation was observed also in WAT of cold-exposed mice, where it was accompanied by a strong increase in NADP(H) levels. Accordingly, NAD kinase and glucose-6-phosphate dehydrogenase activities were enhanced in WAT (but not in BAT). Increased NAD+ levels were observed in BAT/WAT from CD38-/- compared with wild type mice, in line with CD38 being a major NAD+-consumer in AT. CD38-/- mice kept at 6 °C had higher levels of Ucp1 and Pgc-1α in BAT and WAT, and increased levels of phosphorylated hormone-sensitive lipase in BAT, compared with wild type mice. These results demonstrate that CD38, by modulating cellular NAD(P)+ levels, is involved in the regulation of thermogenic responses in cold-activated BAT and WAT.
U2 - 10.1016/j.bbalip.2020.158819
DO - 10.1016/j.bbalip.2020.158819
M3 - SCORING: Journal article
C2 - 33010451
VL - 1866
SP - 158819
JO - BBA-MOL CELL BIOL L
JF - BBA-MOL CELL BIOL L
SN - 1388-1981
IS - 1
ER -