CCND1 amplification and cyclin D1 immunohistochemical expression in head and neck squamous cell carcinomas
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CCND1 amplification and cyclin D1 immunohistochemical expression in head and neck squamous cell carcinomas. / Hanken, Henning; Gröbe, Alexander; Cachovan, Georg; Smeets, Ralf; Simon, Ronald; Sauter, Guido; Heiland, Max; Blessmann, Marco.
in: CLIN ORAL INVEST, Jahrgang 18, Nr. 1, 01.01.2014, S. 269-76.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - CCND1 amplification and cyclin D1 immunohistochemical expression in head and neck squamous cell carcinomas
AU - Hanken, Henning
AU - Gröbe, Alexander
AU - Cachovan, Georg
AU - Smeets, Ralf
AU - Simon, Ronald
AU - Sauter, Guido
AU - Heiland, Max
AU - Blessmann, Marco
PY - 2014/1/1
Y1 - 2014/1/1
N2 - OBJECTIVES: Gene products, which show a significant association to cell proliferation and cell cycle control, are of high scientific interest, because genes as well as gene products could be possible targets for a specific therapeutic approach and eventually be prognostic markers.MATERIALS AND METHODS: Cyclin D1 expression and amplification as well as the Ki-67 expression status were examined in a two tissue microarray analysis for head and neck squamous cell carcinoma (HNSCC) including 546 patients. A tumour site-specific analysis and a survival analysis of 222 oral squamous cell carcinoma (OSCC) patients were performed. Cyclin D1 amplification status was examined with fluorescence in situ hybridisation analysis, while cyclin D1 expression and Ki-67 expression status were examined with IHC.RESULTS: Amplification of the CCND1 gene and immunohistochemical expression of cyclin D1 and Ki-67 were examined in 546 tumours of the head and neck region in two tissue microarrays. CCND1 amplification was significantly more frequent in pharyngeal carcinomas (63%) than in laryngeal (37%) and oral (25%) carcinomas. Among the 222 cases of OSCCs, both CCND1 amplification and cyclin D1 expression were significantly associated with overall survival of the patients (p = 0.0127 and p = 0.0004, respectively). Ki-67 expression was significantly associated with cyclin D1 expression and with amplification of the CCND1 gene (p = 0.0002 and p = 0.0015, respectively) but not with patient overall survival.CONCLUSION: Our results suggest the prognostic value of CCND1 amplification and cyclin D1 expression for patients with OSCC and highlight the genetic differences in HNSCC of different subanatomic localisation.CLINICAL RELEVANCE: Cyclin D1 expression and CCND1 amplification seem to have a prognostic value for OSCC. Further studies of HNSCC should always consider subanatomic genetic differences.
AB - OBJECTIVES: Gene products, which show a significant association to cell proliferation and cell cycle control, are of high scientific interest, because genes as well as gene products could be possible targets for a specific therapeutic approach and eventually be prognostic markers.MATERIALS AND METHODS: Cyclin D1 expression and amplification as well as the Ki-67 expression status were examined in a two tissue microarray analysis for head and neck squamous cell carcinoma (HNSCC) including 546 patients. A tumour site-specific analysis and a survival analysis of 222 oral squamous cell carcinoma (OSCC) patients were performed. Cyclin D1 amplification status was examined with fluorescence in situ hybridisation analysis, while cyclin D1 expression and Ki-67 expression status were examined with IHC.RESULTS: Amplification of the CCND1 gene and immunohistochemical expression of cyclin D1 and Ki-67 were examined in 546 tumours of the head and neck region in two tissue microarrays. CCND1 amplification was significantly more frequent in pharyngeal carcinomas (63%) than in laryngeal (37%) and oral (25%) carcinomas. Among the 222 cases of OSCCs, both CCND1 amplification and cyclin D1 expression were significantly associated with overall survival of the patients (p = 0.0127 and p = 0.0004, respectively). Ki-67 expression was significantly associated with cyclin D1 expression and with amplification of the CCND1 gene (p = 0.0002 and p = 0.0015, respectively) but not with patient overall survival.CONCLUSION: Our results suggest the prognostic value of CCND1 amplification and cyclin D1 expression for patients with OSCC and highlight the genetic differences in HNSCC of different subanatomic localisation.CLINICAL RELEVANCE: Cyclin D1 expression and CCND1 amplification seem to have a prognostic value for OSCC. Further studies of HNSCC should always consider subanatomic genetic differences.
U2 - 10.1007/s00784-013-0967-6
DO - 10.1007/s00784-013-0967-6
M3 - SCORING: Journal article
C2 - 23494454
VL - 18
SP - 269
EP - 276
JO - CLIN ORAL INVEST
JF - CLIN ORAL INVEST
SN - 1432-6981
IS - 1
ER -