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Catch and Release: rare cell analysis from a functionalised medical wire

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Catch and Release: rare cell analysis from a functionalised medical wire. / Chen, Shukun; El-Heliebi, Amin; Tauber, Gerlinde; Langsenlehner, Tanja; Pötscher, Michaela; Kashofer, Karl; Czyż, Zbigniew T; Polzer, Bernhard; Riethdorf, Sabine; Kuske, Andra; Leitinger, Gerd; Pantel, Klaus; Kroneis, Thomas; Sedlmayr, Peter.

in: SCI REP-UK, Jahrgang 7, 24.02.2017, S. 43424.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Chen, S, El-Heliebi, A, Tauber, G, Langsenlehner, T, Pötscher, M, Kashofer, K, Czyż, ZT, Polzer, B, Riethdorf, S, Kuske, A, Leitinger, G, Pantel, K, Kroneis, T & Sedlmayr, P 2017, 'Catch and Release: rare cell analysis from a functionalised medical wire', SCI REP-UK, Jg. 7, S. 43424. https://doi.org/10.1038/srep43424

APA

Chen, S., El-Heliebi, A., Tauber, G., Langsenlehner, T., Pötscher, M., Kashofer, K., Czyż, Z. T., Polzer, B., Riethdorf, S., Kuske, A., Leitinger, G., Pantel, K., Kroneis, T., & Sedlmayr, P. (2017). Catch and Release: rare cell analysis from a functionalised medical wire. SCI REP-UK, 7, 43424. https://doi.org/10.1038/srep43424

Vancouver

Chen S, El-Heliebi A, Tauber G, Langsenlehner T, Pötscher M, Kashofer K et al. Catch and Release: rare cell analysis from a functionalised medical wire. SCI REP-UK. 2017 Feb 24;7:43424. https://doi.org/10.1038/srep43424

Bibtex

@article{949c6b4342514d60a77c3a55f7f0b9c3,
title = "Catch and Release: rare cell analysis from a functionalised medical wire",
abstract = "Enumeration and especially molecular characterization of circulating tumour cells (CTCs) holds great promise for cancer management. We tested a modified type of an in vivo enrichment device (Catch&Release) for its ability to bind and detach cancer cells for the purpose of single-cell molecular downstream analysis in vitro. The evaluation showed that single-cell analysis using array comparative genome hybridization (array-CGH) and next generation sequencing (NGS) is feasible. We found array-CGH to be less noisy when whole genome amplification (WGA) was performed with Ampli1 as compared to GenomePlex (DLRS values 0.65 vs. 1.39). Moreover, Ampli1-processed cells allowed detection of smaller aberrations (median 14.0 vs. 49.9 Mb). Single-cell NGS data obtained from Ampli1-processed samples showed the expected non-synonymous mutations (deletion/SNP) according to bulk DNA. We conclude that clinical application of this refined in vivo enrichment device allows CTC enumeration and characterization, thus, representing a promising tool for personalized medicine.",
keywords = "Journal Article",
author = "Shukun Chen and Amin El-Heliebi and Gerlinde Tauber and Tanja Langsenlehner and Michaela P{\"o}tscher and Karl Kashofer and Czy{\.z}, {Zbigniew T} and Bernhard Polzer and Sabine Riethdorf and Andra Kuske and Gerd Leitinger and Klaus Pantel and Thomas Kroneis and Peter Sedlmayr",
year = "2017",
month = feb,
day = "24",
doi = "10.1038/srep43424",
language = "English",
volume = "7",
pages = "43424",
journal = "SCI REP-UK",
issn = "2045-2322",
publisher = "NATURE PUBLISHING GROUP",

}

RIS

TY - JOUR

T1 - Catch and Release: rare cell analysis from a functionalised medical wire

AU - Chen, Shukun

AU - El-Heliebi, Amin

AU - Tauber, Gerlinde

AU - Langsenlehner, Tanja

AU - Pötscher, Michaela

AU - Kashofer, Karl

AU - Czyż, Zbigniew T

AU - Polzer, Bernhard

AU - Riethdorf, Sabine

AU - Kuske, Andra

AU - Leitinger, Gerd

AU - Pantel, Klaus

AU - Kroneis, Thomas

AU - Sedlmayr, Peter

PY - 2017/2/24

Y1 - 2017/2/24

N2 - Enumeration and especially molecular characterization of circulating tumour cells (CTCs) holds great promise for cancer management. We tested a modified type of an in vivo enrichment device (Catch&Release) for its ability to bind and detach cancer cells for the purpose of single-cell molecular downstream analysis in vitro. The evaluation showed that single-cell analysis using array comparative genome hybridization (array-CGH) and next generation sequencing (NGS) is feasible. We found array-CGH to be less noisy when whole genome amplification (WGA) was performed with Ampli1 as compared to GenomePlex (DLRS values 0.65 vs. 1.39). Moreover, Ampli1-processed cells allowed detection of smaller aberrations (median 14.0 vs. 49.9 Mb). Single-cell NGS data obtained from Ampli1-processed samples showed the expected non-synonymous mutations (deletion/SNP) according to bulk DNA. We conclude that clinical application of this refined in vivo enrichment device allows CTC enumeration and characterization, thus, representing a promising tool for personalized medicine.

AB - Enumeration and especially molecular characterization of circulating tumour cells (CTCs) holds great promise for cancer management. We tested a modified type of an in vivo enrichment device (Catch&Release) for its ability to bind and detach cancer cells for the purpose of single-cell molecular downstream analysis in vitro. The evaluation showed that single-cell analysis using array comparative genome hybridization (array-CGH) and next generation sequencing (NGS) is feasible. We found array-CGH to be less noisy when whole genome amplification (WGA) was performed with Ampli1 as compared to GenomePlex (DLRS values 0.65 vs. 1.39). Moreover, Ampli1-processed cells allowed detection of smaller aberrations (median 14.0 vs. 49.9 Mb). Single-cell NGS data obtained from Ampli1-processed samples showed the expected non-synonymous mutations (deletion/SNP) according to bulk DNA. We conclude that clinical application of this refined in vivo enrichment device allows CTC enumeration and characterization, thus, representing a promising tool for personalized medicine.

KW - Journal Article

U2 - 10.1038/srep43424

DO - 10.1038/srep43424

M3 - SCORING: Journal article

C2 - 28233867

VL - 7

SP - 43424

JO - SCI REP-UK

JF - SCI REP-UK

SN - 2045-2322

ER -