Cardiac fibroblasts support cardiac inflammation in heart failure
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Cardiac fibroblasts support cardiac inflammation in heart failure. / Lindner, Diana; Zietsch, Christin; Tank, Juliane; Sossalla, Samuel; Fluschnik, Nina; Hinrichs, Svenja; Maier, Lars; Poller, Wolfgang; Blankenberg, Stefan; Schultheiss, Heinz-Peter; Tschöpe, Carsten; Westermann, Dirk.
in: BASIC RES CARDIOL, Jahrgang 109, Nr. 5, 2014, S. 428.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Cardiac fibroblasts support cardiac inflammation in heart failure
AU - Lindner, Diana
AU - Zietsch, Christin
AU - Tank, Juliane
AU - Sossalla, Samuel
AU - Fluschnik, Nina
AU - Hinrichs, Svenja
AU - Maier, Lars
AU - Poller, Wolfgang
AU - Blankenberg, Stefan
AU - Schultheiss, Heinz-Peter
AU - Tschöpe, Carsten
AU - Westermann, Dirk
PY - 2014
Y1 - 2014
N2 - Cardiac remodeling and inflammation are hallmarks of cardiac failure and correlate with outcome in patients. However, the basis for the development of both remains unclear. We have previously reported that cardiac inflammation triggers transdifferentiation of fibroblasts to myofibroblasts and therefore increase accumulation of cardiac collagen, one key pathology in cardiac remodeling. Hence, identifying key pathways for inflammation would be beneficial for patients suffering from heart failure also. Besides their well-characterized function in matrix regulation, we here investigate the role of fibroblasts in the inflammatory process. We address for the first time the role of fibroblasts as inflammatory supporter cells in heart failure. Using endomyocardial biopsies from patients with heart failure and dilated cardiomyopathy, we created a primary human cardiac fibroblast cell culture system. We found that mechanical stretch mimicking cardiac dilation in heart failure induces activation of fibroblasts and not only stimulates production of extracellular matrix but more interestingly up-regulates chemokine production and triggers typical inflammatory pathways in vitro. Moreover, the cell culture supernatant of stretched fibroblasts activates inflammatory cells and induces further recruitment of monocytes by allowing transendothelial migration into the cardiac tissue. Our findings reveal that cardiac fibroblasts provide pro-inflammatory mediators and may act as sentinel cells activated by mechanical stress. Those cells are able to recruit inflammatory cells into the cardiac tissue, a process known to aggravate outcome of patients. This might be important in different forms of heart failure and therefore may be one general mechanism specific for fibroblasts.
AB - Cardiac remodeling and inflammation are hallmarks of cardiac failure and correlate with outcome in patients. However, the basis for the development of both remains unclear. We have previously reported that cardiac inflammation triggers transdifferentiation of fibroblasts to myofibroblasts and therefore increase accumulation of cardiac collagen, one key pathology in cardiac remodeling. Hence, identifying key pathways for inflammation would be beneficial for patients suffering from heart failure also. Besides their well-characterized function in matrix regulation, we here investigate the role of fibroblasts in the inflammatory process. We address for the first time the role of fibroblasts as inflammatory supporter cells in heart failure. Using endomyocardial biopsies from patients with heart failure and dilated cardiomyopathy, we created a primary human cardiac fibroblast cell culture system. We found that mechanical stretch mimicking cardiac dilation in heart failure induces activation of fibroblasts and not only stimulates production of extracellular matrix but more interestingly up-regulates chemokine production and triggers typical inflammatory pathways in vitro. Moreover, the cell culture supernatant of stretched fibroblasts activates inflammatory cells and induces further recruitment of monocytes by allowing transendothelial migration into the cardiac tissue. Our findings reveal that cardiac fibroblasts provide pro-inflammatory mediators and may act as sentinel cells activated by mechanical stress. Those cells are able to recruit inflammatory cells into the cardiac tissue, a process known to aggravate outcome of patients. This might be important in different forms of heart failure and therefore may be one general mechanism specific for fibroblasts.
KW - Animals
KW - Cells, Cultured
KW - Fibroblasts/immunology
KW - Heart Failure/immunology
KW - Humans
KW - Inflammation/immunology
KW - Mice
KW - Mice, Inbred C57BL
KW - Myocardium/cytology
KW - Stress, Mechanical
U2 - 10.1007/s00395-014-0428-7
DO - 10.1007/s00395-014-0428-7
M3 - SCORING: Journal article
C2 - 25086637
VL - 109
SP - 428
JO - BASIC RES CARDIOL
JF - BASIC RES CARDIOL
SN - 0300-8428
IS - 5
ER -