Cancer stem cell-like cells derived from malignant peripheral nerve sheath tumors.
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Cancer stem cell-like cells derived from malignant peripheral nerve sheath tumors. / Spyra, Melanie; Kluwe, Lan; Hagel, Christian; Nguyen, Rosa; Panse, Jens; Kurtz, Andreas; Mautner, Viktor Felix; Rabkin, Samuel David; Demestre, Maria.
in: PLOS ONE, Jahrgang 6, Nr. 6, 6, 2011, S. 21099.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Cancer stem cell-like cells derived from malignant peripheral nerve sheath tumors.
AU - Spyra, Melanie
AU - Kluwe, Lan
AU - Hagel, Christian
AU - Nguyen, Rosa
AU - Panse, Jens
AU - Kurtz, Andreas
AU - Mautner, Viktor Felix
AU - Rabkin, Samuel David
AU - Demestre, Maria
PY - 2011
Y1 - 2011
N2 - This study aims to examine whether or not cancer stem cells exist in malignant peripheral nerve sheath tumors (MPNST). Cells of established lines, primary cultures and freshly dissected tumors were cultured in serum free conditions supplemented with epidermal and fibroblast growth factors. From one established human MPNST cell line, S462, cells meeting the criteria for cancer stem cells were isolated. Clonal spheres were obtained, which could be passaged multiple times. Enrichment of stem cell-like cells in these spheres was also supported by increased expression of stem cell markers such as CD133, Oct4, Nestin and NGFR, and decreased expression of mature cell markers such as CD90 and NCAM. Furthermore, cells of these clonal S462 spheres differentiated into Schwann cells, smooth muscle/fibroblast and neurons-like cells under specific differentiation-inducing cultural conditions. Finally, subcutaneous injection of the spheres into immunodeficient nude mice led to tumor formation at a higher rate compared to the parental adherent cells (66% versus 10% at 2.5 × 10(5)). These results provide evidence for the existence of cancer stem cell-like cells in malignant peripheral nerve sheath tumors.
AB - This study aims to examine whether or not cancer stem cells exist in malignant peripheral nerve sheath tumors (MPNST). Cells of established lines, primary cultures and freshly dissected tumors were cultured in serum free conditions supplemented with epidermal and fibroblast growth factors. From one established human MPNST cell line, S462, cells meeting the criteria for cancer stem cells were isolated. Clonal spheres were obtained, which could be passaged multiple times. Enrichment of stem cell-like cells in these spheres was also supported by increased expression of stem cell markers such as CD133, Oct4, Nestin and NGFR, and decreased expression of mature cell markers such as CD90 and NCAM. Furthermore, cells of these clonal S462 spheres differentiated into Schwann cells, smooth muscle/fibroblast and neurons-like cells under specific differentiation-inducing cultural conditions. Finally, subcutaneous injection of the spheres into immunodeficient nude mice led to tumor formation at a higher rate compared to the parental adherent cells (66% versus 10% at 2.5 × 10(5)). These results provide evidence for the existence of cancer stem cell-like cells in malignant peripheral nerve sheath tumors.
KW - Animals
KW - Humans
KW - Mice
KW - Up-Regulation
KW - Cell Line, Tumor
KW - Cell Proliferation
KW - Cell Differentiation
KW - Down-Regulation
KW - Cell Shape
KW - Neoplastic Stem Cells/metabolism/pathology
KW - Nerve Sheath Neoplasms/pathology
KW - Tumor Markers, Biological/genetics
KW - Animals
KW - Humans
KW - Mice
KW - Up-Regulation
KW - Cell Line, Tumor
KW - Cell Proliferation
KW - Cell Differentiation
KW - Down-Regulation
KW - Cell Shape
KW - Neoplastic Stem Cells/metabolism/pathology
KW - Nerve Sheath Neoplasms/pathology
KW - Tumor Markers, Biological/genetics
U2 - 10.1371/journal.pone.0021099
DO - 10.1371/journal.pone.0021099
M3 - SCORING: Journal article
VL - 6
SP - 21099
JO - PLOS ONE
JF - PLOS ONE
SN - 1932-6203
IS - 6
M1 - 6
ER -
