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Cancer cell invasion and EMT marker expression: a three-dimensional study of the human cancer-host interface

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Cancer cell invasion and EMT marker expression: a three-dimensional study of the human cancer-host interface. / Bronsert, P; Enderle-Ammour, K; Bader, M; Timme, S; Kuehs, M; Csanadi, A; Kayser, G; Kohler, I; Bausch, D; Hoeppner, J; Hopt, U T; Keck, T; Stickeler, E; Passlick, B; Schilling, O; Reiss, C P; Vashist, Y; Brabletz, T; Lotz, J; Olesch, J; Werner, M; Wellner, U F.

in: J PATHOL, Jahrgang 234, Nr. 3, 11.2014, S. 410-22.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Bronsert, P, Enderle-Ammour, K, Bader, M, Timme, S, Kuehs, M, Csanadi, A, Kayser, G, Kohler, I, Bausch, D, Hoeppner, J, Hopt, UT, Keck, T, Stickeler, E, Passlick, B, Schilling, O, Reiss, CP, Vashist, Y, Brabletz, T, Lotz, J, Olesch, J, Werner, M & Wellner, UF 2014, 'Cancer cell invasion and EMT marker expression: a three-dimensional study of the human cancer-host interface', J PATHOL, Jg. 234, Nr. 3, S. 410-22. https://doi.org/10.1002/path.4416

APA

Bronsert, P., Enderle-Ammour, K., Bader, M., Timme, S., Kuehs, M., Csanadi, A., Kayser, G., Kohler, I., Bausch, D., Hoeppner, J., Hopt, U. T., Keck, T., Stickeler, E., Passlick, B., Schilling, O., Reiss, C. P., Vashist, Y., Brabletz, T., Lotz, J., ... Wellner, U. F. (2014). Cancer cell invasion and EMT marker expression: a three-dimensional study of the human cancer-host interface. J PATHOL, 234(3), 410-22. https://doi.org/10.1002/path.4416

Vancouver

Bronsert P, Enderle-Ammour K, Bader M, Timme S, Kuehs M, Csanadi A et al. Cancer cell invasion and EMT marker expression: a three-dimensional study of the human cancer-host interface. J PATHOL. 2014 Nov;234(3):410-22. https://doi.org/10.1002/path.4416

Bibtex

@article{23371f55f2584e758b1be56cdfa1edbe,
title = "Cancer cell invasion and EMT marker expression: a three-dimensional study of the human cancer-host interface",
abstract = "Cancer cell invasion takes place at the cancer-host interface and is a prerequisite for distant metastasis. The relationships between current biological and clinical concepts such as cell migration modes, tumour budding and epithelial-mesenchymal transition (EMT) remains unclear in several aspects, especially for the 'real' situation in human cancer. We developed a novel method that provides exact three-dimensional (3D) information on both microscopic morphology and gene expression, over a virtually unlimited spatial range, by reconstruction from serial immunostained tissue slices. Quantitative 3D assessment of tumour budding at the cancer-host interface in human pancreatic, colorectal, lung and breast adenocarcinoma suggests collective cell migration as the mechanism of cancer cell invasion, while single cancer cell migration seems to be virtually absent. Budding tumour cells display a shift towards spindle-like as well as a rounded morphology. This is associated with decreased E-cadherin staining intensity and a shift from membranous to cytoplasmic staining, as well as increased nuclear ZEB1 expression.",
keywords = "Adenocarcinoma, Biomarkers, Tumor, Epithelial-Mesenchymal Transition, Humans, Imaging, Three-Dimensional, Immunohistochemistry, Neoplasm Invasiveness",
author = "P Bronsert and K Enderle-Ammour and M Bader and S Timme and M Kuehs and A Csanadi and G Kayser and I Kohler and D Bausch and J Hoeppner and Hopt, {U T} and T Keck and E Stickeler and B Passlick and O Schilling and Reiss, {C P} and Y Vashist and T Brabletz and J Lotz and J Olesch and M Werner and Wellner, {U F}",
note = "Copyright {\textcopyright} 2014 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.",
year = "2014",
month = nov,
doi = "10.1002/path.4416",
language = "English",
volume = "234",
pages = "410--22",
journal = "J PATHOL",
issn = "0022-3417",
publisher = "John Wiley and Sons Ltd",
number = "3",

}

RIS

TY - JOUR

T1 - Cancer cell invasion and EMT marker expression: a three-dimensional study of the human cancer-host interface

AU - Bronsert, P

AU - Enderle-Ammour, K

AU - Bader, M

AU - Timme, S

AU - Kuehs, M

AU - Csanadi, A

AU - Kayser, G

AU - Kohler, I

AU - Bausch, D

AU - Hoeppner, J

AU - Hopt, U T

AU - Keck, T

AU - Stickeler, E

AU - Passlick, B

AU - Schilling, O

AU - Reiss, C P

AU - Vashist, Y

AU - Brabletz, T

AU - Lotz, J

AU - Olesch, J

AU - Werner, M

AU - Wellner, U F

N1 - Copyright © 2014 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.

PY - 2014/11

Y1 - 2014/11

N2 - Cancer cell invasion takes place at the cancer-host interface and is a prerequisite for distant metastasis. The relationships between current biological and clinical concepts such as cell migration modes, tumour budding and epithelial-mesenchymal transition (EMT) remains unclear in several aspects, especially for the 'real' situation in human cancer. We developed a novel method that provides exact three-dimensional (3D) information on both microscopic morphology and gene expression, over a virtually unlimited spatial range, by reconstruction from serial immunostained tissue slices. Quantitative 3D assessment of tumour budding at the cancer-host interface in human pancreatic, colorectal, lung and breast adenocarcinoma suggests collective cell migration as the mechanism of cancer cell invasion, while single cancer cell migration seems to be virtually absent. Budding tumour cells display a shift towards spindle-like as well as a rounded morphology. This is associated with decreased E-cadherin staining intensity and a shift from membranous to cytoplasmic staining, as well as increased nuclear ZEB1 expression.

AB - Cancer cell invasion takes place at the cancer-host interface and is a prerequisite for distant metastasis. The relationships between current biological and clinical concepts such as cell migration modes, tumour budding and epithelial-mesenchymal transition (EMT) remains unclear in several aspects, especially for the 'real' situation in human cancer. We developed a novel method that provides exact three-dimensional (3D) information on both microscopic morphology and gene expression, over a virtually unlimited spatial range, by reconstruction from serial immunostained tissue slices. Quantitative 3D assessment of tumour budding at the cancer-host interface in human pancreatic, colorectal, lung and breast adenocarcinoma suggests collective cell migration as the mechanism of cancer cell invasion, while single cancer cell migration seems to be virtually absent. Budding tumour cells display a shift towards spindle-like as well as a rounded morphology. This is associated with decreased E-cadherin staining intensity and a shift from membranous to cytoplasmic staining, as well as increased nuclear ZEB1 expression.

KW - Adenocarcinoma

KW - Biomarkers, Tumor

KW - Epithelial-Mesenchymal Transition

KW - Humans

KW - Imaging, Three-Dimensional

KW - Immunohistochemistry

KW - Neoplasm Invasiveness

U2 - 10.1002/path.4416

DO - 10.1002/path.4416

M3 - SCORING: Journal article

C2 - 25081610

VL - 234

SP - 410

EP - 422

JO - J PATHOL

JF - J PATHOL

SN - 0022-3417

IS - 3

ER -