Ca2+ channels in clonal rat anterior pituitary cells (GH3/B6).
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Ca2+ channels in clonal rat anterior pituitary cells (GH3/B6). / Glassmeier, Günter; Hauber, M; Wulfsen, I; Weinsberg, F; Bauer, Christiane K.; Schwarz, J R.
in: PFLUG ARCH EUR J PHY, Jahrgang 442, Nr. 4, 4, 2001, S. 577-587.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - Ca2+ channels in clonal rat anterior pituitary cells (GH3/B6).
AU - Glassmeier, Günter
AU - Hauber, M
AU - Wulfsen, I
AU - Weinsberg, F
AU - Bauer, Christiane K.
AU - Schwarz, J R
PY - 2001
Y1 - 2001
N2 - In clonal rat somatomammotroph cells (GH3/ B6) Ca2+ influx through voltage-dependent Ca2+ channels is important for regulating the Ca2+ concentration that mediates hormone secretion. To study the Ca2+ channel subtypes in GH3/B6 cells, Ca2+ channel currents were recorded with the whole-cell configuration of the patch-clamp technique using Ba2+ as the charge carrier. Forty-nine percent of the total Ba2+ current amplitude was mediated by a nifedipine-sensitive current (L-type). In addition, three other high-voltage-activated Ca2+ channel current components could be distinguished pharmacologically: 10 nM omega-agatoxin-IVA-sensitive current (22%; P-type), omega-conotoxin-MVIIC-sensitive current (18%; Q-type), and toxin-resistant current (24%). Since omega-conotoxin GVIA (2 microM) had no blocking effect, N-type Ca2+ channels are assumed not to be present in GH3/B6 cells. The T-type Ca2+ channel current was either absent or very small. Different pore-forming alpha1 subunits of Ca2+ channels were found to be expressed in GH3/B6 cells, which could be the molecular correlates of the different Ba2+ current subtypes: alpha1G of T-type, alpha1C, alpha1D and alpha1S of L-type, and alpha1A of P/Q-type current. In addition, transcripts for beta1, beta2 and beta3 subunits were detected. Blockage of L-type channels with 10 microM nifedipine or P/Q-type channels with 10 nM omega-agatoxin MVIIC + 200 nM omega-conotoxin blocked action potential firing in GH3/B6 cells and decreased basal prolactin secretion.
AB - In clonal rat somatomammotroph cells (GH3/ B6) Ca2+ influx through voltage-dependent Ca2+ channels is important for regulating the Ca2+ concentration that mediates hormone secretion. To study the Ca2+ channel subtypes in GH3/B6 cells, Ca2+ channel currents were recorded with the whole-cell configuration of the patch-clamp technique using Ba2+ as the charge carrier. Forty-nine percent of the total Ba2+ current amplitude was mediated by a nifedipine-sensitive current (L-type). In addition, three other high-voltage-activated Ca2+ channel current components could be distinguished pharmacologically: 10 nM omega-agatoxin-IVA-sensitive current (22%; P-type), omega-conotoxin-MVIIC-sensitive current (18%; Q-type), and toxin-resistant current (24%). Since omega-conotoxin GVIA (2 microM) had no blocking effect, N-type Ca2+ channels are assumed not to be present in GH3/B6 cells. The T-type Ca2+ channel current was either absent or very small. Different pore-forming alpha1 subunits of Ca2+ channels were found to be expressed in GH3/B6 cells, which could be the molecular correlates of the different Ba2+ current subtypes: alpha1G of T-type, alpha1C, alpha1D and alpha1S of L-type, and alpha1A of P/Q-type current. In addition, transcripts for beta1, beta2 and beta3 subunits were detected. Blockage of L-type channels with 10 microM nifedipine or P/Q-type channels with 10 nM omega-agatoxin MVIIC + 200 nM omega-conotoxin blocked action potential firing in GH3/B6 cells and decreased basal prolactin secretion.
M3 - SCORING: Zeitschriftenaufsatz
VL - 442
SP - 577
EP - 587
JO - PFLUG ARCH EUR J PHY
JF - PFLUG ARCH EUR J PHY
SN - 0031-6768
IS - 4
M1 - 4
ER -