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Bromelain ameliorates hepatic microcirculation after warm ischemia

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Bromelain ameliorates hepatic microcirculation after warm ischemia. / Bahde, Ralf; Palmes, Daniel; Minin, Evgeni; Stratmann, Udo; Diller, Ricarda; Haier, Jörg; Spiegel, Hans-Ullrich.

in: J SURG RES, Jahrgang 139, Nr. 1, 01.05.2007, S. 88-96.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Bahde, R, Palmes, D, Minin, E, Stratmann, U, Diller, R, Haier, J & Spiegel, H-U 2007, 'Bromelain ameliorates hepatic microcirculation after warm ischemia', J SURG RES, Jg. 139, Nr. 1, S. 88-96. https://doi.org/10.1016/j.jss.2006.10.004

APA

Bahde, R., Palmes, D., Minin, E., Stratmann, U., Diller, R., Haier, J., & Spiegel, H-U. (2007). Bromelain ameliorates hepatic microcirculation after warm ischemia. J SURG RES, 139(1), 88-96. https://doi.org/10.1016/j.jss.2006.10.004

Vancouver

Bahde R, Palmes D, Minin E, Stratmann U, Diller R, Haier J et al. Bromelain ameliorates hepatic microcirculation after warm ischemia. J SURG RES. 2007 Mai 1;139(1):88-96. https://doi.org/10.1016/j.jss.2006.10.004

Bibtex

@article{76f8c766a5ba4357a04f7b756561b479,
title = "Bromelain ameliorates hepatic microcirculation after warm ischemia",
abstract = "BACKGROUND: Because of its immunomodulatory action, the protease bromelain represents a novel strategy for the treatment of hepatic ischemia/reperfusion (I/R) injury. A dose-response study was performed to investigate the effect of bromelain on liver function, microcirculation, and leukocyte-endothelium interactions in hepatic I/R injury.MATERIALS AND METHODS: One hundred forty rats were randomized to 8 short-term or 12 long-term groups (n=7 each). A 30 min normothermic hepatic ischemia was induced by Pringle maneuver with a portocaval shunt. Animals were treated 60 min prior to ischemia with either no therapy, 0.1, 1.0, or 10 mg/kg b.w. bromelain i.v. In the short-term experiments, microcirculation was investigated 30 min after sham operation or ischemia using intravital microscopy. In the long-term experiments AST, ALT, and bradykinin levels were determined for 14 d after central venous catheter (CVC) placement only, sham operation, or ischemia. Additionally, apoptosis rate, Kupffer cell activation, endothelial cell damage, and eNOS expression were analyzed.RESULTS: In sham-operated animals, treatment with 10 mg/kg b.w. bromelain led to a disturbed microcirculation with increased leukocyte adherence, apoptosis rate, Kupffer cell activation, and endothelial cell damage. Six h after CVC placement and administration of 10 mg/kg b.w. bromelain, AST and ALT levels were significantly increased. After I/R, rats treated with 0.1 mg/kg b.w. bromelain showed an improved microcirculation, reduction in leukocyte adhesion, apoptosis rates, Kupffer cell activation and endothelial cell damage, increased eNOS expression, and significantly lower AST levels compared with untreated animals.CONCLUSION: Bromelain represents a novel approach to the treatment of hepatic I/R injury with a limited therapeutic window.",
keywords = "Animals, Bradykinin, Bromelains, Cytokines, Dose-Response Relationship, Drug, Female, Immunohistochemistry, Ischemia, Kupffer Cells, Liver Circulation, Microscopy, Rats, Rats, Wistar",
author = "Ralf Bahde and Daniel Palmes and Evgeni Minin and Udo Stratmann and Ricarda Diller and J{\"o}rg Haier and Hans-Ullrich Spiegel",
year = "2007",
month = may,
day = "1",
doi = "10.1016/j.jss.2006.10.004",
language = "English",
volume = "139",
pages = "88--96",
journal = "J SURG RES",
issn = "0022-4804",
publisher = "Academic Press Inc.",
number = "1",

}

RIS

TY - JOUR

T1 - Bromelain ameliorates hepatic microcirculation after warm ischemia

AU - Bahde, Ralf

AU - Palmes, Daniel

AU - Minin, Evgeni

AU - Stratmann, Udo

AU - Diller, Ricarda

AU - Haier, Jörg

AU - Spiegel, Hans-Ullrich

PY - 2007/5/1

Y1 - 2007/5/1

N2 - BACKGROUND: Because of its immunomodulatory action, the protease bromelain represents a novel strategy for the treatment of hepatic ischemia/reperfusion (I/R) injury. A dose-response study was performed to investigate the effect of bromelain on liver function, microcirculation, and leukocyte-endothelium interactions in hepatic I/R injury.MATERIALS AND METHODS: One hundred forty rats were randomized to 8 short-term or 12 long-term groups (n=7 each). A 30 min normothermic hepatic ischemia was induced by Pringle maneuver with a portocaval shunt. Animals were treated 60 min prior to ischemia with either no therapy, 0.1, 1.0, or 10 mg/kg b.w. bromelain i.v. In the short-term experiments, microcirculation was investigated 30 min after sham operation or ischemia using intravital microscopy. In the long-term experiments AST, ALT, and bradykinin levels were determined for 14 d after central venous catheter (CVC) placement only, sham operation, or ischemia. Additionally, apoptosis rate, Kupffer cell activation, endothelial cell damage, and eNOS expression were analyzed.RESULTS: In sham-operated animals, treatment with 10 mg/kg b.w. bromelain led to a disturbed microcirculation with increased leukocyte adherence, apoptosis rate, Kupffer cell activation, and endothelial cell damage. Six h after CVC placement and administration of 10 mg/kg b.w. bromelain, AST and ALT levels were significantly increased. After I/R, rats treated with 0.1 mg/kg b.w. bromelain showed an improved microcirculation, reduction in leukocyte adhesion, apoptosis rates, Kupffer cell activation and endothelial cell damage, increased eNOS expression, and significantly lower AST levels compared with untreated animals.CONCLUSION: Bromelain represents a novel approach to the treatment of hepatic I/R injury with a limited therapeutic window.

AB - BACKGROUND: Because of its immunomodulatory action, the protease bromelain represents a novel strategy for the treatment of hepatic ischemia/reperfusion (I/R) injury. A dose-response study was performed to investigate the effect of bromelain on liver function, microcirculation, and leukocyte-endothelium interactions in hepatic I/R injury.MATERIALS AND METHODS: One hundred forty rats were randomized to 8 short-term or 12 long-term groups (n=7 each). A 30 min normothermic hepatic ischemia was induced by Pringle maneuver with a portocaval shunt. Animals were treated 60 min prior to ischemia with either no therapy, 0.1, 1.0, or 10 mg/kg b.w. bromelain i.v. In the short-term experiments, microcirculation was investigated 30 min after sham operation or ischemia using intravital microscopy. In the long-term experiments AST, ALT, and bradykinin levels were determined for 14 d after central venous catheter (CVC) placement only, sham operation, or ischemia. Additionally, apoptosis rate, Kupffer cell activation, endothelial cell damage, and eNOS expression were analyzed.RESULTS: In sham-operated animals, treatment with 10 mg/kg b.w. bromelain led to a disturbed microcirculation with increased leukocyte adherence, apoptosis rate, Kupffer cell activation, and endothelial cell damage. Six h after CVC placement and administration of 10 mg/kg b.w. bromelain, AST and ALT levels were significantly increased. After I/R, rats treated with 0.1 mg/kg b.w. bromelain showed an improved microcirculation, reduction in leukocyte adhesion, apoptosis rates, Kupffer cell activation and endothelial cell damage, increased eNOS expression, and significantly lower AST levels compared with untreated animals.CONCLUSION: Bromelain represents a novel approach to the treatment of hepatic I/R injury with a limited therapeutic window.

KW - Animals

KW - Bradykinin

KW - Bromelains

KW - Cytokines

KW - Dose-Response Relationship, Drug

KW - Female

KW - Immunohistochemistry

KW - Ischemia

KW - Kupffer Cells

KW - Liver Circulation

KW - Microscopy

KW - Rats

KW - Rats, Wistar

U2 - 10.1016/j.jss.2006.10.004

DO - 10.1016/j.jss.2006.10.004

M3 - SCORING: Journal article

C2 - 17292418

VL - 139

SP - 88

EP - 96

JO - J SURG RES

JF - J SURG RES

SN - 0022-4804

IS - 1

ER -