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Brain-Derived Extracellular Vesicles in Health and Disease: A Methodological Perspective

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Brain-Derived Extracellular Vesicles in Health and Disease: A Methodological Perspective. / Brenna, Santra; Krisp, Christoph; Altmeppen, Hermann Clemens; Magnus, Tim; Puig, Berta.

in: INT J MOL SCI, Jahrgang 22, Nr. 3, 29.01.2021, S. 1365.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Review › Forschung

Harvard

Brenna, S, Krisp, C, Altmeppen, HC, Magnus, T & Puig, B 2021, 'Brain-Derived Extracellular Vesicles in Health and Disease: A Methodological Perspective', INT J MOL SCI, Jg. 22, Nr. 3, S. 1365. https://doi.org/10.3390/ijms22031365

APA

Brenna, S., Krisp, C., Altmeppen, H. C., Magnus, T., & Puig, B. (2021). Brain-Derived Extracellular Vesicles in Health and Disease: A Methodological Perspective. INT J MOL SCI, 22(3), 1365. https://doi.org/10.3390/ijms22031365

Vancouver

Brenna S, Krisp C, Altmeppen HC, Magnus T, Puig B. Brain-Derived Extracellular Vesicles in Health and Disease: A Methodological Perspective. INT J MOL SCI. 2021 Jan 29;22(3):1365. https://doi.org/10.3390/ijms22031365

Bibtex

@article{7c200af1c09b4d37abefaddcf832bd1a,
title = "Brain-Derived Extracellular Vesicles in Health and Disease: A Methodological Perspective",
abstract = "Extracellular vesicles (EVs) are double membrane structures released by presumably all cell types that transport and deliver lipids, proteins, and genetic material to near or distant recipient cells, thereby affecting their phenotype. The basic knowledge of their functions in healthy and diseased brain is still murky and many questions about their biology are unsolved. In neurological diseases, EVs are regarded as attractive biomarkers and as therapeutic tools due to their ability to cross the blood-brain barrier (BBB). EVs have been successfully isolated from conditioned media of primary brain cells and cerebrospinal fluid (CSF), but protocols allowing for the direct study of pathophysiological events mediated or influenced by EVs isolated from brain have only recently been published. This review aims to give a brief overview of the current knowledge of EVs' functions in the central nervous system (CNS) and the current protocols to isolate brain-derived EVs (BDEVs) used in different publications. By comparing the proteomic analysis of some of these publications, we also assess the influence of the isolation method on the protein content of BDEVs.",
author = "Santra Brenna and Christoph Krisp and Altmeppen, {Hermann Clemens} and Tim Magnus and Berta Puig",
year = "2021",
month = jan,
day = "29",
doi = "10.3390/ijms22031365",
language = "English",
volume = "22",
pages = "1365",
journal = "INT J MOL SCI",
issn = "1661-6596",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "3",

}

RIS

TY - JOUR

T1 - Brain-Derived Extracellular Vesicles in Health and Disease: A Methodological Perspective

AU - Brenna, Santra

AU - Krisp, Christoph

AU - Altmeppen, Hermann Clemens

AU - Magnus, Tim

AU - Puig, Berta

PY - 2021/1/29

Y1 - 2021/1/29

N2 - Extracellular vesicles (EVs) are double membrane structures released by presumably all cell types that transport and deliver lipids, proteins, and genetic material to near or distant recipient cells, thereby affecting their phenotype. The basic knowledge of their functions in healthy and diseased brain is still murky and many questions about their biology are unsolved. In neurological diseases, EVs are regarded as attractive biomarkers and as therapeutic tools due to their ability to cross the blood-brain barrier (BBB). EVs have been successfully isolated from conditioned media of primary brain cells and cerebrospinal fluid (CSF), but protocols allowing for the direct study of pathophysiological events mediated or influenced by EVs isolated from brain have only recently been published. This review aims to give a brief overview of the current knowledge of EVs' functions in the central nervous system (CNS) and the current protocols to isolate brain-derived EVs (BDEVs) used in different publications. By comparing the proteomic analysis of some of these publications, we also assess the influence of the isolation method on the protein content of BDEVs.

AB - Extracellular vesicles (EVs) are double membrane structures released by presumably all cell types that transport and deliver lipids, proteins, and genetic material to near or distant recipient cells, thereby affecting their phenotype. The basic knowledge of their functions in healthy and diseased brain is still murky and many questions about their biology are unsolved. In neurological diseases, EVs are regarded as attractive biomarkers and as therapeutic tools due to their ability to cross the blood-brain barrier (BBB). EVs have been successfully isolated from conditioned media of primary brain cells and cerebrospinal fluid (CSF), but protocols allowing for the direct study of pathophysiological events mediated or influenced by EVs isolated from brain have only recently been published. This review aims to give a brief overview of the current knowledge of EVs' functions in the central nervous system (CNS) and the current protocols to isolate brain-derived EVs (BDEVs) used in different publications. By comparing the proteomic analysis of some of these publications, we also assess the influence of the isolation method on the protein content of BDEVs.

U2 - 10.3390/ijms22031365

DO - 10.3390/ijms22031365

M3 - SCORING: Review article

C2 - 33573018

VL - 22

SP - 1365

JO - INT J MOL SCI

JF - INT J MOL SCI

SN - 1661-6596

IS - 3

ER -