Bone marrow-derived progenitor cells do not contribute to podocyte turnover in the puromycin aminoglycoside and renal ablation models in rats.
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Bone marrow-derived progenitor cells do not contribute to podocyte turnover in the puromycin aminoglycoside and renal ablation models in rats. / Meyer-Schwesinger, Catherine; Lange, Claudia; Bröcker, Verena; Agustian, Putri Andina; Putri, Andina Agustian; Lehmann, Ulrich; Raabe, Annette; Brinkmeyer, Martina; Kobayashi, Eiji; Schiffer, Mario; Büsche, Guntram; Kreipe, Hans H; Thaiss, Friedrich; Becker, Jan U.
in: AM J PATHOL, Jahrgang 178, Nr. 2, 2, 2011, S. 494-499.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Bone marrow-derived progenitor cells do not contribute to podocyte turnover in the puromycin aminoglycoside and renal ablation models in rats.
AU - Meyer-Schwesinger, Catherine
AU - Lange, Claudia
AU - Bröcker, Verena
AU - Agustian, Putri Andina
AU - Putri, Andina Agustian
AU - Lehmann, Ulrich
AU - Raabe, Annette
AU - Brinkmeyer, Martina
AU - Kobayashi, Eiji
AU - Schiffer, Mario
AU - Büsche, Guntram
AU - Kreipe, Hans H
AU - Thaiss, Friedrich
AU - Becker, Jan U
PY - 2011
Y1 - 2011
N2 - A key event in the progression of glomerular disease is podocyte loss that leads to focal and segmental glomerulosclerosis (FSGS). Because adult podocytes are postmitotic cells, podocyte replacement by bone marrow-derived progenitors could prevent podocytopenia and FSGS. This study uses double immunofluorescence for Wilms' tumor-1 and enhanced green fluorescent protein (eGFP) to examine whether an eGFP-positive bone marrow transplant can replace podocytes under normal circumstances and in 3 different rat models of FSGS: puromycin aminoglycoside nephropathy, subtotal nephrectomy, and uninephrectomy. Bone marrow engraftment was successful, with more than 70% eGFP-positive cells and virtually normal histologic findings. No bone marrow transplant-derived podocytes were found in four control rats after transplantation, in nine rats at up to 10 weeks after puromycin aminoglycoside nephropathy induction, in three rats 23 days after subtotal nephrectomy, and in six rats up to 21 days after uninephrectomy. A total of 2200 glomeruli with 14,474 podocytes were evaluated in all groups. Thus, podocyte replacement by bone marrow-derived cells does not contribute to podocyte turnover in rats, even in models of podocyte damage. This is in contrast to previous studies in mice, in which bone marrow-derived podocytes were found. Further studies will address this discrepancy, which could be explained by species differences or by predominant podocyte regeneration from a parietal epithelial cell niche.
AB - A key event in the progression of glomerular disease is podocyte loss that leads to focal and segmental glomerulosclerosis (FSGS). Because adult podocytes are postmitotic cells, podocyte replacement by bone marrow-derived progenitors could prevent podocytopenia and FSGS. This study uses double immunofluorescence for Wilms' tumor-1 and enhanced green fluorescent protein (eGFP) to examine whether an eGFP-positive bone marrow transplant can replace podocytes under normal circumstances and in 3 different rat models of FSGS: puromycin aminoglycoside nephropathy, subtotal nephrectomy, and uninephrectomy. Bone marrow engraftment was successful, with more than 70% eGFP-positive cells and virtually normal histologic findings. No bone marrow transplant-derived podocytes were found in four control rats after transplantation, in nine rats at up to 10 weeks after puromycin aminoglycoside nephropathy induction, in three rats 23 days after subtotal nephrectomy, and in six rats up to 21 days after uninephrectomy. A total of 2200 glomeruli with 14,474 podocytes were evaluated in all groups. Thus, podocyte replacement by bone marrow-derived cells does not contribute to podocyte turnover in rats, even in models of podocyte damage. This is in contrast to previous studies in mice, in which bone marrow-derived podocytes were found. Further studies will address this discrepancy, which could be explained by species differences or by predominant podocyte regeneration from a parietal epithelial cell niche.
KW - Animals
KW - Female
KW - Disease Models, Animal
KW - Rats
KW - Flow Cytometry
KW - Fluorescent Antibody Technique
KW - Rats, Wistar
KW - Ablation Techniques
KW - Bone Marrow Cells/cytology
KW - Glomerulosclerosis, Focal Segmental/pathology/surgery
KW - Kidney/pathology/surgery
KW - Kidney Diseases/pathology/surgery
KW - Podocytes/cytology/metabolism
KW - Puromycin
KW - Stem Cells/cytology/metabolism
KW - Animals
KW - Female
KW - Disease Models, Animal
KW - Rats
KW - Flow Cytometry
KW - Fluorescent Antibody Technique
KW - Rats, Wistar
KW - Ablation Techniques
KW - Bone Marrow Cells/cytology
KW - Glomerulosclerosis, Focal Segmental/pathology/surgery
KW - Kidney/pathology/surgery
KW - Kidney Diseases/pathology/surgery
KW - Podocytes/cytology/metabolism
KW - Puromycin
KW - Stem Cells/cytology/metabolism
M3 - SCORING: Journal article
VL - 178
SP - 494
EP - 499
JO - AM J PATHOL
JF - AM J PATHOL
SN - 0002-9440
IS - 2
M1 - 2
ER -
