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BK(Ca) channel activation by membrane-associated cGMP kinase may contribute to uterine quiescence in pregnancy

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BK(Ca) channel activation by membrane-associated cGMP kinase may contribute to uterine quiescence in pregnancy. / Zhou, Xiao-Bo; Wang, G X; Ruth, P; Hüneke, Bernd; Korth, M.

in: AM J PHYSIOL-CELL PH, Jahrgang 279, Nr. 6, 6, 2000, S. 1751-1759.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Zhou, X-B, Wang, GX, Ruth, P, Hüneke, B & Korth, M 2000, 'BK(Ca) channel activation by membrane-associated cGMP kinase may contribute to uterine quiescence in pregnancy', AM J PHYSIOL-CELL PH, Jg. 279, Nr. 6, 6, S. 1751-1759. https://doi.org/10.1152/ajpcell.2000.279.6.C1751

APA

Zhou, X-B., Wang, G. X., Ruth, P., Hüneke, B., & Korth, M. (2000). BK(Ca) channel activation by membrane-associated cGMP kinase may contribute to uterine quiescence in pregnancy. AM J PHYSIOL-CELL PH, 279(6), 1751-1759. [6]. https://doi.org/10.1152/ajpcell.2000.279.6.C1751

Vancouver

Zhou X-B, Wang GX, Ruth P, Hüneke B, Korth M. BK(Ca) channel activation by membrane-associated cGMP kinase may contribute to uterine quiescence in pregnancy. AM J PHYSIOL-CELL PH. 2000;279(6):1751-1759. 6. https://doi.org/10.1152/ajpcell.2000.279.6.C1751

Bibtex

@article{429a3d83f3594445ba8a27312c635496,
title = "BK(Ca) channel activation by membrane-associated cGMP kinase may contribute to uterine quiescence in pregnancy",
abstract = "We investigated the influence of pregnancy on large-conductance calcium-activated potassium channel (BK(Ca)) activity (NP(o)) and on channel expression in membranes of isolated human myometrial smooth muscle cells. NP(o) in inside-out patches was higher in pregnant myometria (PM) compared with nonpregnant myometria (NPM), and the half-maximal activation potential was shifted by 39 mV to more negative potentials. This effect was not due to an enhanced BK(Ca) channel expression. In the presence of cAMP kinase (PKA) or cGMP kinase (PKG), NP(o) increased in patches from PM but decreased in those from NPM. Western blot analysis and use of a specific PKG inhibitor (1 microM KT-5823) verified the existence of a partially active membrane-associated PKG. Inhibition of PKA by 100 nM PKI, the inhibitory peptide of PKA, had no effect on NP(o). 8-p-Chlorophenylthio-cGMP (8-pCPT-cGMP) hyperpolarized cells from PM. This effect was abolished by iberiotoxin, a specific blocker of BK(Ca) channels. It is concluded that an endogenous, membrane-bound PKG in myometrial cells specifically enhances BK(Ca) channel activity during pregnancy and thus may contribute to uterine quiescence during pregnancy.",
author = "Xiao-Bo Zhou and Wang, {G X} and P Ruth and Bernd H{\"u}neke and M Korth",
year = "2000",
doi = "10.1152/ajpcell.2000.279.6.C1751",
language = "Deutsch",
volume = "279",
pages = "1751--1759",
journal = "AM J PHYSIOL-CELL PH",
issn = "0363-6143",
publisher = "American Physiological Society",
number = "6",

}

RIS

TY - JOUR

T1 - BK(Ca) channel activation by membrane-associated cGMP kinase may contribute to uterine quiescence in pregnancy

AU - Zhou, Xiao-Bo

AU - Wang, G X

AU - Ruth, P

AU - Hüneke, Bernd

AU - Korth, M

PY - 2000

Y1 - 2000

N2 - We investigated the influence of pregnancy on large-conductance calcium-activated potassium channel (BK(Ca)) activity (NP(o)) and on channel expression in membranes of isolated human myometrial smooth muscle cells. NP(o) in inside-out patches was higher in pregnant myometria (PM) compared with nonpregnant myometria (NPM), and the half-maximal activation potential was shifted by 39 mV to more negative potentials. This effect was not due to an enhanced BK(Ca) channel expression. In the presence of cAMP kinase (PKA) or cGMP kinase (PKG), NP(o) increased in patches from PM but decreased in those from NPM. Western blot analysis and use of a specific PKG inhibitor (1 microM KT-5823) verified the existence of a partially active membrane-associated PKG. Inhibition of PKA by 100 nM PKI, the inhibitory peptide of PKA, had no effect on NP(o). 8-p-Chlorophenylthio-cGMP (8-pCPT-cGMP) hyperpolarized cells from PM. This effect was abolished by iberiotoxin, a specific blocker of BK(Ca) channels. It is concluded that an endogenous, membrane-bound PKG in myometrial cells specifically enhances BK(Ca) channel activity during pregnancy and thus may contribute to uterine quiescence during pregnancy.

AB - We investigated the influence of pregnancy on large-conductance calcium-activated potassium channel (BK(Ca)) activity (NP(o)) and on channel expression in membranes of isolated human myometrial smooth muscle cells. NP(o) in inside-out patches was higher in pregnant myometria (PM) compared with nonpregnant myometria (NPM), and the half-maximal activation potential was shifted by 39 mV to more negative potentials. This effect was not due to an enhanced BK(Ca) channel expression. In the presence of cAMP kinase (PKA) or cGMP kinase (PKG), NP(o) increased in patches from PM but decreased in those from NPM. Western blot analysis and use of a specific PKG inhibitor (1 microM KT-5823) verified the existence of a partially active membrane-associated PKG. Inhibition of PKA by 100 nM PKI, the inhibitory peptide of PKA, had no effect on NP(o). 8-p-Chlorophenylthio-cGMP (8-pCPT-cGMP) hyperpolarized cells from PM. This effect was abolished by iberiotoxin, a specific blocker of BK(Ca) channels. It is concluded that an endogenous, membrane-bound PKG in myometrial cells specifically enhances BK(Ca) channel activity during pregnancy and thus may contribute to uterine quiescence during pregnancy.

U2 - 10.1152/ajpcell.2000.279.6.C1751

DO - 10.1152/ajpcell.2000.279.6.C1751

M3 - SCORING: Zeitschriftenaufsatz

VL - 279

SP - 1751

EP - 1759

JO - AM J PHYSIOL-CELL PH

JF - AM J PHYSIOL-CELL PH

SN - 0363-6143

IS - 6

M1 - 6

ER -