Bioluminescence imaging for assessment of immune responses following implantation of engineered heart tissue (EHT).
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Bioluminescence imaging for assessment of immune responses following implantation of engineered heart tissue (EHT). / Conradi, Lenard; Pahrmann, Christiane; Schmidt, Stephanie; Deuse, Tobias; Hansen, Arne; Eder, Alexandra; Reichenspurner, Hermann; Robbins, Robert C; Eschenhagen, Thomas; Schrepfer, Sonja.
in: JOVE-J VIS EXP, Jahrgang 52, 2011, S. 2605.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Bioluminescence imaging for assessment of immune responses following implantation of engineered heart tissue (EHT).
AU - Conradi, Lenard
AU - Pahrmann, Christiane
AU - Schmidt, Stephanie
AU - Deuse, Tobias
AU - Hansen, Arne
AU - Eder, Alexandra
AU - Reichenspurner, Hermann
AU - Robbins, Robert C
AU - Eschenhagen, Thomas
AU - Schrepfer, Sonja
PY - 2011
Y1 - 2011
N2 - Various techniques of cardiac tissue engineering have been pursued in the past decades including scaffolding strategies using either native or bioartificial scaffold materials, entrapment of cardiac myocytes in hydrogels such as fibrin or collagen and stacking of myocyte monolayers. These concepts aim at restoration of compromised cardiac function (e.g. after myocardial infarction) or as experimental models (e.g. predictive toxicology and substance screening or disease modelling). Precise monitoring of cell survival after implantation of engineered heart tissue (EHT) has now become possible using in-vivo bioluminescence imaging (BLI) techniques. Here we describe the generation of fibrin-based EHT from a transgenic rat strain with ubiquitous expression of firefly luciferase (ROSA/luciferase-LEW Tg; ). Implantation is performed into the greater omentum of different rat strains to assess immune responses of the recipient organism following EHT implantation. Comparison of results generated by BLI and the Enzyme Linked Immuno Spot Technique (ELISPOT) confirm the usability of BLI for the assessment of immune responses.
AB - Various techniques of cardiac tissue engineering have been pursued in the past decades including scaffolding strategies using either native or bioartificial scaffold materials, entrapment of cardiac myocytes in hydrogels such as fibrin or collagen and stacking of myocyte monolayers. These concepts aim at restoration of compromised cardiac function (e.g. after myocardial infarction) or as experimental models (e.g. predictive toxicology and substance screening or disease modelling). Precise monitoring of cell survival after implantation of engineered heart tissue (EHT) has now become possible using in-vivo bioluminescence imaging (BLI) techniques. Here we describe the generation of fibrin-based EHT from a transgenic rat strain with ubiquitous expression of firefly luciferase (ROSA/luciferase-LEW Tg; ). Implantation is performed into the greater omentum of different rat strains to assess immune responses of the recipient organism following EHT implantation. Comparison of results generated by BLI and the Enzyme Linked Immuno Spot Technique (ELISPOT) confirm the usability of BLI for the assessment of immune responses.
KW - Animals
KW - Rats
KW - Tissue Engineering/methods
KW - Fibrin/chemistry
KW - Heart Transplantation/methods
KW - Luciferases, Firefly/biosynthesis/genetics
KW - Luminescent Measurements/methods
KW - Muscle Cells/immunology/metabolism/physiology/transplantation
KW - Myocardium/cytology/immunology/metabolism
KW - Rats, Transgenic
KW - Tissue Transplantation/methods
KW - Animals
KW - Rats
KW - Tissue Engineering/methods
KW - Fibrin/chemistry
KW - Heart Transplantation/methods
KW - Luciferases, Firefly/biosynthesis/genetics
KW - Luminescent Measurements/methods
KW - Muscle Cells/immunology/metabolism/physiology/transplantation
KW - Myocardium/cytology/immunology/metabolism
KW - Rats, Transgenic
KW - Tissue Transplantation/methods
M3 - SCORING: Journal article
VL - 52
SP - 2605
JO - JOVE-J VIS EXP
JF - JOVE-J VIS EXP
SN - 1940-087X
ER -
