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Biological and molecular characterization of a new human ampullary cancer cell line

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Biological and molecular characterization of a new human ampullary cancer cell line. / Peiper, Matthias; Alldinger, Ingo; Heller, Raoul; Pilarsky, Christian; Schumacher, Udo; Knoefel, Wolfram-Trudo; Heinecke, Antje; Izbicki, Jakob R.

in: ANTICANCER RES, Jahrgang 23, Nr. 1A, 12.04.2003, S. 291-8.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Peiper, M, Alldinger, I, Heller, R, Pilarsky, C, Schumacher, U, Knoefel, W-T, Heinecke, A & Izbicki, JR 2003, 'Biological and molecular characterization of a new human ampullary cancer cell line', ANTICANCER RES, Jg. 23, Nr. 1A, S. 291-8.

APA

Peiper, M., Alldinger, I., Heller, R., Pilarsky, C., Schumacher, U., Knoefel, W-T., Heinecke, A., & Izbicki, J. R. (2003). Biological and molecular characterization of a new human ampullary cancer cell line. ANTICANCER RES, 23(1A), 291-8.

Vancouver

Peiper M, Alldinger I, Heller R, Pilarsky C, Schumacher U, Knoefel W-T et al. Biological and molecular characterization of a new human ampullary cancer cell line. ANTICANCER RES. 2003 Apr 12;23(1A):291-8.

Bibtex

@article{fca2c7b22ff54e93ac5ee8145eccefde,
title = "Biological and molecular characterization of a new human ampullary cancer cell line",
abstract = "BACKGROUND: Ampullary carcinoma of the pancreas accounts for 5% of all gastrointestinal malignancies in humans. Only a very few cell lines of this carcinoma have been established.PATIENTS AND METHODS: Tumor cells isolated from a surgically resected ampullary carcinoma were put into culture. The cultured cells were characterized for their biological, immunological and molecular properties including in vitra and in viva cell kinetics, karyotype, expression of tumor markers and lysis by autologous cytotoxic T-lymphocytes.RESULTS: An ampullary cancer cell line, designated UKEAC-99, was established. It proliferates as a monolayer with a doubling-time of 29 hours. The cytological features of the cultured and of the xenografted cells from SCID mice were similar to those of the primary tumor. UKEAC-99 cells were lysed by autologous cytotoxic T-lymphocytes in a HLA-class I restricted fashion. Hybridization of tumor mRNA to a dedicated DNA-chip revealed overexpression of several genes involved in tumor progression such as L6, Matrilysin and Vimentin. Tumor suppressor genes and apoptosis-associated genes like p73 or IL1 alpha are expressed at a low level.CONCLUSION: We established a new ampullary carcinoma cell line, which is rare and may contribute to our understanding of the biological behavior of ampullary carcinoma. The lysis by autologous cytotoxic T-lymphocytes and possibly shared antigens with other pancreatic cancers may help to identify tumor-associated/tumor-specific antigens. The detailed analysis of gene expression allows researchers new insights into ampullary cancer that can be exploited in future in vitro and in vivo models.",
keywords = "Ampulla of Vater, Animals, Common Bile Duct Neoplasms, Gene Expression Profiling, Histocompatibility Antigens Class I, Humans, Karyotyping, Lymphocytes, Tumor-Infiltrating, Male, Mice, Mice, SCID, Middle Aged, Neoplasm Transplantation, Oligonucleotide Array Sequence Analysis, T-Lymphocytes, Cytotoxic, Transplantation, Heterologous, Tumor Cells, Cultured",
author = "Matthias Peiper and Ingo Alldinger and Raoul Heller and Christian Pilarsky and Udo Schumacher and Wolfram-Trudo Knoefel and Antje Heinecke and Izbicki, {Jakob R}",
year = "2003",
month = apr,
day = "12",
language = "English",
volume = "23",
pages = "291--8",
journal = "ANTICANCER RES",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
number = "1A",

}

RIS

TY - JOUR

T1 - Biological and molecular characterization of a new human ampullary cancer cell line

AU - Peiper, Matthias

AU - Alldinger, Ingo

AU - Heller, Raoul

AU - Pilarsky, Christian

AU - Schumacher, Udo

AU - Knoefel, Wolfram-Trudo

AU - Heinecke, Antje

AU - Izbicki, Jakob R

PY - 2003/4/12

Y1 - 2003/4/12

N2 - BACKGROUND: Ampullary carcinoma of the pancreas accounts for 5% of all gastrointestinal malignancies in humans. Only a very few cell lines of this carcinoma have been established.PATIENTS AND METHODS: Tumor cells isolated from a surgically resected ampullary carcinoma were put into culture. The cultured cells were characterized for their biological, immunological and molecular properties including in vitra and in viva cell kinetics, karyotype, expression of tumor markers and lysis by autologous cytotoxic T-lymphocytes.RESULTS: An ampullary cancer cell line, designated UKEAC-99, was established. It proliferates as a monolayer with a doubling-time of 29 hours. The cytological features of the cultured and of the xenografted cells from SCID mice were similar to those of the primary tumor. UKEAC-99 cells were lysed by autologous cytotoxic T-lymphocytes in a HLA-class I restricted fashion. Hybridization of tumor mRNA to a dedicated DNA-chip revealed overexpression of several genes involved in tumor progression such as L6, Matrilysin and Vimentin. Tumor suppressor genes and apoptosis-associated genes like p73 or IL1 alpha are expressed at a low level.CONCLUSION: We established a new ampullary carcinoma cell line, which is rare and may contribute to our understanding of the biological behavior of ampullary carcinoma. The lysis by autologous cytotoxic T-lymphocytes and possibly shared antigens with other pancreatic cancers may help to identify tumor-associated/tumor-specific antigens. The detailed analysis of gene expression allows researchers new insights into ampullary cancer that can be exploited in future in vitro and in vivo models.

AB - BACKGROUND: Ampullary carcinoma of the pancreas accounts for 5% of all gastrointestinal malignancies in humans. Only a very few cell lines of this carcinoma have been established.PATIENTS AND METHODS: Tumor cells isolated from a surgically resected ampullary carcinoma were put into culture. The cultured cells were characterized for their biological, immunological and molecular properties including in vitra and in viva cell kinetics, karyotype, expression of tumor markers and lysis by autologous cytotoxic T-lymphocytes.RESULTS: An ampullary cancer cell line, designated UKEAC-99, was established. It proliferates as a monolayer with a doubling-time of 29 hours. The cytological features of the cultured and of the xenografted cells from SCID mice were similar to those of the primary tumor. UKEAC-99 cells were lysed by autologous cytotoxic T-lymphocytes in a HLA-class I restricted fashion. Hybridization of tumor mRNA to a dedicated DNA-chip revealed overexpression of several genes involved in tumor progression such as L6, Matrilysin and Vimentin. Tumor suppressor genes and apoptosis-associated genes like p73 or IL1 alpha are expressed at a low level.CONCLUSION: We established a new ampullary carcinoma cell line, which is rare and may contribute to our understanding of the biological behavior of ampullary carcinoma. The lysis by autologous cytotoxic T-lymphocytes and possibly shared antigens with other pancreatic cancers may help to identify tumor-associated/tumor-specific antigens. The detailed analysis of gene expression allows researchers new insights into ampullary cancer that can be exploited in future in vitro and in vivo models.

KW - Ampulla of Vater

KW - Animals

KW - Common Bile Duct Neoplasms

KW - Gene Expression Profiling

KW - Histocompatibility Antigens Class I

KW - Humans

KW - Karyotyping

KW - Lymphocytes, Tumor-Infiltrating

KW - Male

KW - Mice

KW - Mice, SCID

KW - Middle Aged

KW - Neoplasm Transplantation

KW - Oligonucleotide Array Sequence Analysis

KW - T-Lymphocytes, Cytotoxic

KW - Transplantation, Heterologous

KW - Tumor Cells, Cultured

M3 - SCORING: Journal article

C2 - 12680226

VL - 23

SP - 291

EP - 298

JO - ANTICANCER RES

JF - ANTICANCER RES

SN - 0250-7005

IS - 1A

ER -