Assessment of microRNAs in patients with unstable angina pectoris
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Assessment of microRNAs in patients with unstable angina pectoris. / Zeller, Tanja; Keller, Till; Ojeda, Francisco; Reichlin, Tobias; Twerenbold, Raphael; Tzikas, Stergios; Wild, Philipp S; Reiter, Miriam; Czyz, Ewa; Lackner, Karl J; Munzel, Thomas; Mueller, Christian; Blankenberg, Stefan.
in: EUR HEART J, Jahrgang 35, Nr. 31, 14.08.2014, S. 2106-2114.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Assessment of microRNAs in patients with unstable angina pectoris
AU - Zeller, Tanja
AU - Keller, Till
AU - Ojeda, Francisco
AU - Reichlin, Tobias
AU - Twerenbold, Raphael
AU - Tzikas, Stergios
AU - Wild, Philipp S
AU - Reiter, Miriam
AU - Czyz, Ewa
AU - Lackner, Karl J
AU - Munzel, Thomas
AU - Mueller, Christian
AU - Blankenberg, Stefan
N1 - Published on behalf of the European Society of Cardiology. All rights reserved. © The Author 2014. For permissions please email: journals.permissions@oup.com.
PY - 2014/8/14
Y1 - 2014/8/14
N2 - AIMS: While cardiac troponin measurements have significantly improved the early diagnosis of myocardial infarction, the timely biomarker-based diagnosis of unstable angina pectoris (UAP) remains a major unmet clinical challenge. The aim of this study was to assess levels of circulating microRNAs (miRNAs) as possible novel biomarkers in patients with UAP.METHODS AND RESULTS: A three-phase approach was conducted, comprising (i) profiling of miRNAs in patients with UAP and controls groups; (ii) replication of significant miRNAs in an independent patient cohort, (iii) validation of a multi-miRNAs panel in a third cohort. Out of 25 miRNAs selected for replication, 8 miRNAs remained significantly associated with UAP. In a validation phase, a miRNA panel including miR-132, miR-150, and miR-186 showed the highest discriminatory power [area under the receiver-operating-characteristic curve (AUC): 0.91; CI: 0.84-0.98].CONCLUSION: Using a profiling-replication-validation model, we identified eight miRNAs, which may facilitate the diagnosis of UAP.
AB - AIMS: While cardiac troponin measurements have significantly improved the early diagnosis of myocardial infarction, the timely biomarker-based diagnosis of unstable angina pectoris (UAP) remains a major unmet clinical challenge. The aim of this study was to assess levels of circulating microRNAs (miRNAs) as possible novel biomarkers in patients with UAP.METHODS AND RESULTS: A three-phase approach was conducted, comprising (i) profiling of miRNAs in patients with UAP and controls groups; (ii) replication of significant miRNAs in an independent patient cohort, (iii) validation of a multi-miRNAs panel in a third cohort. Out of 25 miRNAs selected for replication, 8 miRNAs remained significantly associated with UAP. In a validation phase, a miRNA panel including miR-132, miR-150, and miR-186 showed the highest discriminatory power [area under the receiver-operating-characteristic curve (AUC): 0.91; CI: 0.84-0.98].CONCLUSION: Using a profiling-replication-validation model, we identified eight miRNAs, which may facilitate the diagnosis of UAP.
KW - Adult
KW - Aged
KW - Angina, Unstable/diagnosis
KW - Case-Control Studies
KW - Early Diagnosis
KW - Female
KW - Genetic Markers
KW - Genetic Techniques
KW - Humans
KW - Male
KW - MicroRNAs/metabolism
KW - Middle Aged
KW - Myocardial Infarction/diagnosis
KW - ROC Curve
KW - Reproducibility of Results
U2 - 10.1093/eurheartj/ehu151
DO - 10.1093/eurheartj/ehu151
M3 - SCORING: Journal article
C2 - 24727883
VL - 35
SP - 2106
EP - 2114
JO - EUR HEART J
JF - EUR HEART J
SN - 0195-668X
IS - 31
ER -
