Assembly and structural analysis of a covalently closed nano-scale DNA cage.

TY - JOUR

T1 - Assembly and structural analysis of a covalently closed nano-scale DNA cage.

AU - Andersen, Felicie F

AU - Knudsen, Bjarne

AU - Oliveira, Cristiano Luis Pinto

AU - Frøhlich, Rikke F

AU - Krüger, Dinna

AU - Bungert, Jörg

AU - Agbandje-McKenna, Mavis

AU - McKenna, Robert

AU - Juul, Sissel

AU - Veigaard, Christopher

AU - Koch, Jørn

AU - Rubinstein, John L

AU - Guldbrandtsen, Bernt

AU - Hede, Marianne S

AU - Karlsson, Göran

AU - Andersen, Anni H

AU - Pedersen, Jan Skov

AU - Knudsen, Birgitta R

PY - 2008

Y1 - 2008

N2 - The inherent properties of DNA as a stable polymer with unique affinity for partner molecules determined by the specific Watson-Crick base pairing makes it an ideal component in self-assembling structures. This has been exploited for decades in the design of a variety of artificial substrates for investigations of DNA-interacting enzymes. More recently, strategies for synthesis of more complex two-dimensional (2D) and 3D DNA structures have emerged. However, the building of such structures is still in progress and more experiences from different research groups and different fields of expertise are necessary before complex DNA structures can be routinely designed for the use in basal science and/or biotechnology. Here we present the design, construction and structural analysis of a covalently closed and stable 3D DNA structure with the connectivity of an octahedron, as defined by the double-stranded DNA helices that assembles from eight oligonucleotides with a yield of approximately 30%. As demonstrated by Small Angle X-ray Scattering and cryo-Transmission Electron Microscopy analyses the eight-stranded DNA structure has a central cavity larger than the apertures in the surrounding DNA lattice and can be described as a nano-scale DNA cage, Hence, in theory it could hold proteins or other bio-molecules to enable their investigation in certain harmful environments or even allow their organization into higher order structures.

AB - The inherent properties of DNA as a stable polymer with unique affinity for partner molecules determined by the specific Watson-Crick base pairing makes it an ideal component in self-assembling structures. This has been exploited for decades in the design of a variety of artificial substrates for investigations of DNA-interacting enzymes. More recently, strategies for synthesis of more complex two-dimensional (2D) and 3D DNA structures have emerged. However, the building of such structures is still in progress and more experiences from different research groups and different fields of expertise are necessary before complex DNA structures can be routinely designed for the use in basal science and/or biotechnology. Here we present the design, construction and structural analysis of a covalently closed and stable 3D DNA structure with the connectivity of an octahedron, as defined by the double-stranded DNA helices that assembles from eight oligonucleotides with a yield of approximately 30%. As demonstrated by Small Angle X-ray Scattering and cryo-Transmission Electron Microscopy analyses the eight-stranded DNA structure has a central cavity larger than the apertures in the surrounding DNA lattice and can be described as a nano-scale DNA cage, Hence, in theory it could hold proteins or other bio-molecules to enable their investigation in certain harmful environments or even allow their organization into higher order structures.

M3 - SCORING: Zeitschriftenaufsatz

VL - 36

SP - 1113

EP - 1119

JO - NUCLEIC ACIDS RES

JF - NUCLEIC ACIDS RES

SN - 0305-1048

IS - 4

M1 - 4

ER -