Application of stable carbon isotope analysis to the detection of testosterone administration to cattle.
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Application of stable carbon isotope analysis to the detection of testosterone administration to cattle. / Hebestreit, Moritz; Flenker, Ulrich; Buisson, Corinne; Andre, Francois; Bruno, Le Bizec; Fry, Hildburg; Lang, Melanie; Weigert, Angelika Preiss; Heinrich, Katharina; Hird, Simon; Schänzer, Wilhelm.
in: J AGR FOOD CHEM, Jahrgang 54, Nr. 8, 8, 2006, S. 2850-2858.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Application of stable carbon isotope analysis to the detection of testosterone administration to cattle.
AU - Hebestreit, Moritz
AU - Flenker, Ulrich
AU - Buisson, Corinne
AU - Andre, Francois
AU - Bruno, Le Bizec
AU - Fry, Hildburg
AU - Lang, Melanie
AU - Weigert, Angelika Preiss
AU - Heinrich, Katharina
AU - Hird, Simon
AU - Schänzer, Wilhelm
PY - 2006
Y1 - 2006
N2 - The use of anabolic substances is prohibited in food-producing animals throughout the European Union. No method is available to reliably detect the misuse of natural hormones in cattle. A method was developed to detect the abuse of testosterone in cattle fattening. Synthesized testosterone is rather depleted in the (13)C/(12)C ratio. Hence, the method is based on gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) analysis of urine. To select testosterone metabolites and endogenous reference compounds (ERC), the concentration of urinary steroids of cattle was investigated. Dehydroepiandrosterone and androst-5ene-3beta,17alpha-diol were chosen as ERCs to show endogenous (13)C/(12)C ratios. Etiocholanolone and 5alpha-androstane-3beta,17alpha-diol were chosen as the most important testosterone metabolites. Other metabolites known from literature like epitestosterone were less promising. In principle, GC/C/IRMS is a nonspecific method because finally carbon dioxide is analyzed. Therefore, a dedicated cleanup procedure for the selected steroids was developed. By means of proposed confidence intervals in the isotopic composition of ERCs and metabolites, the administration of testosterone to cattle could be detected reliably. Differences of up to 11 per thousand on the delta-scale between ERC and testosterone metabolites were found after testosterone administration, whereas endogenous differences did not exceed 2 per thousand.
AB - The use of anabolic substances is prohibited in food-producing animals throughout the European Union. No method is available to reliably detect the misuse of natural hormones in cattle. A method was developed to detect the abuse of testosterone in cattle fattening. Synthesized testosterone is rather depleted in the (13)C/(12)C ratio. Hence, the method is based on gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) analysis of urine. To select testosterone metabolites and endogenous reference compounds (ERC), the concentration of urinary steroids of cattle was investigated. Dehydroepiandrosterone and androst-5ene-3beta,17alpha-diol were chosen as ERCs to show endogenous (13)C/(12)C ratios. Etiocholanolone and 5alpha-androstane-3beta,17alpha-diol were chosen as the most important testosterone metabolites. Other metabolites known from literature like epitestosterone were less promising. In principle, GC/C/IRMS is a nonspecific method because finally carbon dioxide is analyzed. Therefore, a dedicated cleanup procedure for the selected steroids was developed. By means of proposed confidence intervals in the isotopic composition of ERCs and metabolites, the administration of testosterone to cattle could be detected reliably. Differences of up to 11 per thousand on the delta-scale between ERC and testosterone metabolites were found after testosterone administration, whereas endogenous differences did not exceed 2 per thousand.
M3 - SCORING: Zeitschriftenaufsatz
VL - 54
SP - 2850
EP - 2858
JO - J AGR FOOD CHEM
JF - J AGR FOOD CHEM
SN - 0021-8561
IS - 8
M1 - 8
ER -