Apolipoprotein E-dependent inverse regulation of vertebral bone and adipose tissue mass in C57Bl/6 mice: modulation by diet-induced obesity.
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Apolipoprotein E-dependent inverse regulation of vertebral bone and adipose tissue mass in C57Bl/6 mice: modulation by diet-induced obesity. / Bartelt, Alexander; Beil, Frank Timo; Schinke, Thorsten; Röser, Kerstin; Ruether, Wolfgang; Heeren, Jörg; Niemeier, Andreas.
in: BONE, Jahrgang 47, Nr. 4, 4, 01.10.2010, S. 736-745.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Apolipoprotein E-dependent inverse regulation of vertebral bone and adipose tissue mass in C57Bl/6 mice: modulation by diet-induced obesity.
AU - Bartelt, Alexander
AU - Beil, Frank Timo
AU - Schinke, Thorsten
AU - Röser, Kerstin
AU - Ruether, Wolfgang
AU - Heeren, Jörg
AU - Niemeier, Andreas
N1 - Copyright © 2010 Elsevier Inc. All rights reserved.
PY - 2010/10/1
Y1 - 2010/10/1
N2 - The long prevailing view that obesity is generally associated with beneficial effects on the skeleton has recently been challenged. Apolipoprotein E (apoE) is known to influence both adipose tissue and bone. The goal of the current study was to examine the impact of apoE on the development of fat mass and bone mass in mice under conditions of diet-induced obesity (DIO). Four week-old male C57BL/6 (WT) and apoE-deficient (apoE(-/-)) mice received a control or a diabetogenic high-fat diet (HFD) for 16 weeks. The control-fed apoE(-/-) animals displayed less total fat mass and higher lumbar trabecular bone volume (BV/TV) than WT controls. When stressed with HFD to induce obesity, apoE(-/-) mice had a lower body weight, lower serum glucose, insulin and leptin levels and accumulated less white adipose tissue mass at all sites including bone marrow. While WT animals showed no significant change in BV/TV and bone formation rate (BFR), apoE deficiency led to a decrease of BV/TV and BFR when stressed with HFD. Bone resorption parameters were not affected by HFD in either genotype. Taken together, under normal dietary conditions, apoE-deficient mice acquire less fat mass and more bone mass than WT littermates. When stressed with HFD to develop DIO, the difference of total body fat mass becomes larger and the difference of bone mass smaller between the genotypes. We conclude that apoE is involved in an inverse regulation of bone mass and fat mass in growing mice and that this effect is modulated by diet-induced obesity.
AB - The long prevailing view that obesity is generally associated with beneficial effects on the skeleton has recently been challenged. Apolipoprotein E (apoE) is known to influence both adipose tissue and bone. The goal of the current study was to examine the impact of apoE on the development of fat mass and bone mass in mice under conditions of diet-induced obesity (DIO). Four week-old male C57BL/6 (WT) and apoE-deficient (apoE(-/-)) mice received a control or a diabetogenic high-fat diet (HFD) for 16 weeks. The control-fed apoE(-/-) animals displayed less total fat mass and higher lumbar trabecular bone volume (BV/TV) than WT controls. When stressed with HFD to induce obesity, apoE(-/-) mice had a lower body weight, lower serum glucose, insulin and leptin levels and accumulated less white adipose tissue mass at all sites including bone marrow. While WT animals showed no significant change in BV/TV and bone formation rate (BFR), apoE deficiency led to a decrease of BV/TV and BFR when stressed with HFD. Bone resorption parameters were not affected by HFD in either genotype. Taken together, under normal dietary conditions, apoE-deficient mice acquire less fat mass and more bone mass than WT littermates. When stressed with HFD to develop DIO, the difference of total body fat mass becomes larger and the difference of bone mass smaller between the genotypes. We conclude that apoE is involved in an inverse regulation of bone mass and fat mass in growing mice and that this effect is modulated by diet-induced obesity.
KW - Adipocytes
KW - Adipose Tissue
KW - Animals
KW - Apolipoproteins E
KW - Blood Glucose
KW - Bone Marrow Cells
KW - Bone Remodeling
KW - Cell Proliferation
KW - Diet
KW - Dietary Fats
KW - Epididymis
KW - Insulin
KW - Leptin
KW - Lipids
KW - Male
KW - Mice
KW - Mice, Inbred C57BL
KW - Obesity
KW - Organ Size
KW - Spine
KW - Subcutaneous Tissue
KW - Viscera
KW - Weight Gain
U2 - 10.1016/j.bone.2010.07.002
DO - 10.1016/j.bone.2010.07.002
M3 - SCORING: Zeitschriftenaufsatz
C2 - 20633710
VL - 47
SP - 736
EP - 745
JO - BONE
JF - BONE
SN - 8756-3282
IS - 4
M1 - 4
ER -
