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Analysis of the matrix-assisted laser desorption ionization-time of flight mass spectrum of Staphylococcus aureus identifies mutations that allow differentiation of the main clonal lineages

Standard

Analysis of the matrix-assisted laser desorption ionization-time of flight mass spectrum of Staphylococcus aureus identifies mutations that allow differentiation of the main clonal lineages. / Josten, Michaele; Reif, Marion; Szekat, Christiane; Al-Sabti, Nahed; Roemer, Terry; Sparbier, Katrin; Kostrzewa, Markus; Rohde, Holger; Sahl, Hans-Georg; Bierbaum, Gabriele.

in: J CLIN MICROBIOL, Jahrgang 51, Nr. 6, 01.06.2013, S. 1809-17.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Josten, M, Reif, M, Szekat, C, Al-Sabti, N, Roemer, T, Sparbier, K, Kostrzewa, M, Rohde, H, Sahl, H-G & Bierbaum, G 2013, 'Analysis of the matrix-assisted laser desorption ionization-time of flight mass spectrum of Staphylococcus aureus identifies mutations that allow differentiation of the main clonal lineages', J CLIN MICROBIOL, Jg. 51, Nr. 6, S. 1809-17. https://doi.org/10.1128/JCM.00518-13

APA

Josten, M., Reif, M., Szekat, C., Al-Sabti, N., Roemer, T., Sparbier, K., Kostrzewa, M., Rohde, H., Sahl, H-G., & Bierbaum, G. (2013). Analysis of the matrix-assisted laser desorption ionization-time of flight mass spectrum of Staphylococcus aureus identifies mutations that allow differentiation of the main clonal lineages. J CLIN MICROBIOL, 51(6), 1809-17. https://doi.org/10.1128/JCM.00518-13

Vancouver

Josten M, Reif M, Szekat C, Al-Sabti N, Roemer T, Sparbier K et al. Analysis of the matrix-assisted laser desorption ionization-time of flight mass spectrum of Staphylococcus aureus identifies mutations that allow differentiation of the main clonal lineages. J CLIN MICROBIOL. 2013 Jun 1;51(6):1809-17. https://doi.org/10.1128/JCM.00518-13

Bibtex

@article{1b19b23285a441b38d7d05af1463db65,
title = "Analysis of the matrix-assisted laser desorption ionization-time of flight mass spectrum of Staphylococcus aureus identifies mutations that allow differentiation of the main clonal lineages",
abstract = "Nosocomial infections involving epidemic methicillin-resistant Staphylococcus aureus (MRSA) strains are a serious problem in many countries. In order to analyze outbreaks, the infectious isolates have to be typed; however, most molecular methods are expensive or labor-intensive. Here, we evaluated matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) of cell extracts for the molecular characterization of S. aureus strains. The peak patterns of 401 MRSA and methicillin-susceptible S. aureus (MSSA) strains, including clinical and laboratory strains, were analyzed. Database searches indicated the peptides that were represented by the corresponding peaks in the spectra. The identities of the peptides were confirmed by the sequencing of mutants, the expression of antisense RNA fragments that resulted in the knockdown of the peptide of interest and the concomitant loss of the signal, or tandem MALDI-TOF MS (MALDI-TOF/TOF MS). It was shown that the signals derive mainly from stress proteins and ribosomal proteins. Peak shifts that differentiate the main S. aureus clonal complexes CC5, CC22, CC8, CC45, CC30, and CC1 correlate to point mutations in the respective genes. Retrospective typing of an MRSA outbreak showed that it is possible to differentiate unrelated MSSA, MRSA, and borderline resistant S. aureus (BORSA) strains isolated from health care workers. In conclusion, this method allows for the detection of the epidemic lineages of S. aureus during species identification by MALDI-TOF MS analysis.",
keywords = "Bacterial Proteins, Bacterial Typing Techniques, Humans, Mutant Proteins, Mutation, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Staphylococcus aureus",
author = "Michaele Josten and Marion Reif and Christiane Szekat and Nahed Al-Sabti and Terry Roemer and Katrin Sparbier and Markus Kostrzewa and Holger Rohde and Hans-Georg Sahl and Gabriele Bierbaum",
year = "2013",
month = jun,
day = "1",
doi = "10.1128/JCM.00518-13",
language = "English",
volume = "51",
pages = "1809--17",
journal = "J CLIN MICROBIOL",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "6",

}

RIS

TY - JOUR

T1 - Analysis of the matrix-assisted laser desorption ionization-time of flight mass spectrum of Staphylococcus aureus identifies mutations that allow differentiation of the main clonal lineages

AU - Josten, Michaele

AU - Reif, Marion

AU - Szekat, Christiane

AU - Al-Sabti, Nahed

AU - Roemer, Terry

AU - Sparbier, Katrin

AU - Kostrzewa, Markus

AU - Rohde, Holger

AU - Sahl, Hans-Georg

AU - Bierbaum, Gabriele

PY - 2013/6/1

Y1 - 2013/6/1

N2 - Nosocomial infections involving epidemic methicillin-resistant Staphylococcus aureus (MRSA) strains are a serious problem in many countries. In order to analyze outbreaks, the infectious isolates have to be typed; however, most molecular methods are expensive or labor-intensive. Here, we evaluated matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) of cell extracts for the molecular characterization of S. aureus strains. The peak patterns of 401 MRSA and methicillin-susceptible S. aureus (MSSA) strains, including clinical and laboratory strains, were analyzed. Database searches indicated the peptides that were represented by the corresponding peaks in the spectra. The identities of the peptides were confirmed by the sequencing of mutants, the expression of antisense RNA fragments that resulted in the knockdown of the peptide of interest and the concomitant loss of the signal, or tandem MALDI-TOF MS (MALDI-TOF/TOF MS). It was shown that the signals derive mainly from stress proteins and ribosomal proteins. Peak shifts that differentiate the main S. aureus clonal complexes CC5, CC22, CC8, CC45, CC30, and CC1 correlate to point mutations in the respective genes. Retrospective typing of an MRSA outbreak showed that it is possible to differentiate unrelated MSSA, MRSA, and borderline resistant S. aureus (BORSA) strains isolated from health care workers. In conclusion, this method allows for the detection of the epidemic lineages of S. aureus during species identification by MALDI-TOF MS analysis.

AB - Nosocomial infections involving epidemic methicillin-resistant Staphylococcus aureus (MRSA) strains are a serious problem in many countries. In order to analyze outbreaks, the infectious isolates have to be typed; however, most molecular methods are expensive or labor-intensive. Here, we evaluated matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) of cell extracts for the molecular characterization of S. aureus strains. The peak patterns of 401 MRSA and methicillin-susceptible S. aureus (MSSA) strains, including clinical and laboratory strains, were analyzed. Database searches indicated the peptides that were represented by the corresponding peaks in the spectra. The identities of the peptides were confirmed by the sequencing of mutants, the expression of antisense RNA fragments that resulted in the knockdown of the peptide of interest and the concomitant loss of the signal, or tandem MALDI-TOF MS (MALDI-TOF/TOF MS). It was shown that the signals derive mainly from stress proteins and ribosomal proteins. Peak shifts that differentiate the main S. aureus clonal complexes CC5, CC22, CC8, CC45, CC30, and CC1 correlate to point mutations in the respective genes. Retrospective typing of an MRSA outbreak showed that it is possible to differentiate unrelated MSSA, MRSA, and borderline resistant S. aureus (BORSA) strains isolated from health care workers. In conclusion, this method allows for the detection of the epidemic lineages of S. aureus during species identification by MALDI-TOF MS analysis.

KW - Bacterial Proteins

KW - Bacterial Typing Techniques

KW - Humans

KW - Mutant Proteins

KW - Mutation

KW - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

KW - Staphylococcus aureus

U2 - 10.1128/JCM.00518-13

DO - 10.1128/JCM.00518-13

M3 - SCORING: Journal article

C2 - 23554199

VL - 51

SP - 1809

EP - 1817

JO - J CLIN MICROBIOL

JF - J CLIN MICROBIOL

SN - 0095-1137

IS - 6

ER -